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Species: Homo sapiens
Genetic Insert: P85(N564K)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40226 Copy
Species: Homo sapiens
Genetic Insert: P85(K550_K562dup)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40224 Copy
Species: Homo sapiens
Genetic Insert: P85(KS459delN)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40222 Copy
Species: Homo sapiens
Genetic Insert: P85(E439del)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40221 Copy
Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:7711; Vector Backbone:pLenti pRRL-SV40(puro)_CMV(mcs); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_40178 Copy
Species: Homo sapiens
Genetic Insert: Artemis
Vector Backbone Description: Backbone Marker:In-house; Backbone Size:4442; Vector Backbone:pUC; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941364
Proper citation: RRID:Addgene_40211 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-3N+S251A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-3N+S251A
Note: During the Addgene quality control process, it was determined that this construct originally encoded a par-3 fragment containing amino acid residues 1 through 408, but a premature stop codon limits the protein to amino acid residue 364.
Proper citation: RRID:Addgene_40160 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-3C
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-3C
Proper citation: RRID:Addgene_40161 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-3N
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-3N
Note: During the Addgene quality control process, it was determined that this construct originally encoded a par-3 fragment containing amino acid residues 1 through 408, but a premature stop codon limits the protein to amino acid residue 364.
Proper citation: RRID:Addgene_40159 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-1C:FLAG
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-1C:FLAG
Proper citation: RRID:Addgene_40158 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, S241A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2RR S241A
Proper citation: RRID:Addgene_40154 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, 7SA
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 7SA
7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A.
Proper citation: RRID:Addgene_40153 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, R163A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2RR R163A
Proper citation: RRID:Addgene_40152 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, K162A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2RR K162A
Proper citation: RRID:Addgene_40151 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (C56S)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR C56S
Proper citation: RRID:Addgene_40146 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R183-5A + 7SA)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R183-5A + 7SA
7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A
Note: A deletion of amino acid residues 502-523 near the C-terminus of the protein was discovered during the Addgene quality control process and is present in this plasmid.
Proper citation: RRID:Addgene_40141 Copy
Genetic Insert: par-2 RNAi resistant (wild-type)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR wild-type
Proper citation: RRID:Addgene_40137 Copy
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747757
Comments: For more information on Pelczar TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#pelczar
Please note that plasmid #40132 is only functional in combination with plasmid #40131.
Proper citation: RRID:Addgene_40132 Copy
Species: Homo sapiens
Genetic Insert: RINT1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7732; Vector Backbone:pLenti6-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23074196
Proper citation: RRID:Addgene_40124 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa244-468)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa245-STOP (RNAi res) +3’UTR
Proper citation: RRID:Addgene_40121 Copy
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