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Species: Homo sapiens
Genetic Insert: HsZNF598-del715-864-siRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Sequence according to sequence given by NCBI: NM_178167.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148872 Copy
Species: Homo sapiens
Genetic Insert: HsZNF598-siRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Sequence according to sequence given by NCBI: NM_178167.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148873 Copy
Species: Homo sapiens
Genetic Insert: HsZNF598-siRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Sequence according to sequence given by NCBI: NM_178167.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148877 Copy
Species: Homo sapiens
Genetic Insert: HsHelz_1051-1942
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31570513
Comments: sequence according to the sequence of NCBI:NM_014877.3 or Uniprot.:P42694 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148807 Copy
Species: Homo sapiens
Genetic Insert: HsHelz-E795QF1107V
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31570513
Comments: sequence according to the sequence of NCBI:NM_014877.3 or Uniprot.:P42694 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148809 Copy
Species: Homo sapiens
Genetic Insert: HsHelz_1-1474
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31570513
Comments: sequence according to the sequence of NCBI:NM_014877.3 or Uniprot.:P42694 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148880 Copy
Species: Homo sapiens
Genetic Insert: HsHelz_1-1474
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31570513
Comments: sequence according to the sequence of NCBI:NM_014877.3 or Uniprot.:P42694 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148898 Copy
Species: Homo sapiens
Genetic Insert: HsHelz_1475-1942
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31570513
Comments: sequence according to the sequence of NCBI:NM_014877.3 or Uniprot.:P42694 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148899 Copy
Species: Synthetic
Genetic Insert: SpdCas9 - SmallBiT
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122471
Comments: This plasmid also contains the coding sequence for the LargeBiT fragment of the NanoBiT split NanoLuc luciferase downstream of the SmallBiT coding sequence, but there is a stopcodon after the strep-tag fused to SmallBiT. Hence, this plasmid encodes for the SpdCas9-SmallBiT protein by default. By cleaving the plasmid with SpeI restriction enzyme, the SmallBiT part may be removed, and after selfligation the plasmid then encodes for the SpCas9-LargeBiT protein (i.e. plasmid #198751 is obtained).
Proper citation: RRID:Addgene_198750 Copy
Species: Synthetic
Genetic Insert: SpdCas9 - LargeBiT
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122471
Proper citation: RRID:Addgene_198751 Copy
Species: Synthetic
Genetic Insert: p15A ORI plus NGS barcode 24
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199054 Copy
Species: Synthetic
Genetic Insert: pBC1 ORI plus NGS barcode 25
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199055 Copy
Species: Synthetic
Genetic Insert: pNG2 ORI plus NGS barcode 22
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199052 Copy
Species: Synthetic
Genetic Insert: pSG5 ORI plus NGS barcode 20
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199050 Copy
Species: Synthetic
Genetic Insert: γω assembly linker
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199058 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199065 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199067 Copy
Species: Synthetic
Genetic Insert: pIP501 ORI plus NGS barcode 4
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199036 Copy
Species: Synthetic
Genetic Insert: pUB110 ORI plus NGS barcode 2
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199034 Copy
Species: Synthetic
Genetic Insert: pAM?1 ORI plus NGS barcode 3
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199035 Copy
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