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Species: Mus musculus
Genetic Insert: KLC1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166962 Copy
Species: Mus musculus
Genetic Insert: sFRP-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16693 Copy
Genetic Insert: MSI2-ADA
Vector Backbone Description: Backbone Marker:Addgene (with XhoI at 5' end and EcoRI at 3'end); Backbone Size:7362; Vector Backbone:MSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32332729
Proper citation: RRID:Addgene_166967 Copy
Species: Synthetic
Genetic Insert: Termination module:PolyU(0)
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166994 Copy
Species: Synthetic
Genetic Insert: Termination module:PolyU(0)
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166992 Copy
Species: Synthetic
Genetic Insert: Termination module:PolyU(4), Hairpin = 0 nt
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166991 Copy
Species: Synthetic
Genetic Insert: Negative control sgRNA 1
Vector Backbone Description: Backbone Marker:Addgene #99375; Backbone Size:9524; Vector Backbone:LentiGuide_Hygro-eGFP; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33112375
Proper citation: RRID:Addgene_166998 Copy
Species: Synthetic
Genetic Insert: Lactate oxidase
Vector Backbone Description: Backbone Size:12266; Vector Backbone:pTYF; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33400321
Proper citation: RRID:Addgene_166919 Copy
Vector Backbone Description: Backbone Size:10332; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD133.63, Ligation number L4671.
Proper citation: RRID:Addgene_1670 Copy
Species: Homo sapiens
Genetic Insert: Termination module:PolyU(1)
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166984 Copy
Species: Synthetic
Genetic Insert: mRFP-FKBP-mSos1-linkercat
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pT2Aneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173858 Copy
Species: Homo sapiens
Genetic Insert: SERPiNA1
Vector Backbone Description: Vector Backbone:pET-8C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28752459
Comments: For details on mutating an internal initiation site in the SERPIINA1 gene (changing the pEAT8 plasmid to pEAT8-137) see: Laska, M. E. (2001) The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli, Thesis, Massachusetts Institute of Technology, Cambridge, MA
Proper citation: RRID:Addgene_173807 Copy
Species: Other
Genetic Insert: Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Vector Backbone Description: Vector Backbone:pET-23b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26092728
Comments: Bacillus thuringiensis PI-PLC (UniProt P08954) cleaves GPI-anchored proteins off the surface of cells and can be used instead of the 97% identical, commercially available B. cereus enzyme (UniProt P14262).
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173808 Copy
Species: Synthetic
Genetic Insert: STRHSTPAB
Vector Backbone Description: Vector Backbone:pST50Tr; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34408191
Proper citation: RRID:Addgene_173827 Copy
Species: Synthetic
Genetic Insert: STRHSTPABPACCBPv3
Vector Backbone Description: Vector Backbone:pST50Trc4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34408191
Proper citation: RRID:Addgene_173829 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 2)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173786 Copy
Species: Rattus norvegicus
Genetic Insert: NZAP
Vector Backbone Description: Backbone Marker:Goff Lab; Backbone Size:0; Vector Backbone:pBabe-HAZ (modified pBabe vector); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215647
Proper citation: RRID:Addgene_17382 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pE-FLP; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Comments: Depositor confirms the S102Y and S279Y in Cre does not affect plasmid function.
The pE_Cre_lambdaCos plasmid can be packaged in lambda phages using the CY2120 cosmid packaging strain [Cronan 2013]. Lambda infection provides a rapid and efficient way to deliver the pE_Cre_lambdaCos plasmid.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_173785 Copy
Species: Other
Genetic Insert: Staphylococcus aureus plc
Vector Backbone Description: Vector Backbone:pET-21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22390775
Comments: See UniProt ID P45723 for the mature, untagged sequence.
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173810 Copy
Genetic Insert: Myc-BirA-G3-ER
Vector Backbone Description: Backbone Marker:Drosophila Genomics Resource Center; Vector Backbone:pWalium10-roe; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33888706
Comments: BirA-G3 sequence obtained from the Ting Lab:
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY. Efficient proximity labeling in living cells and organisms with TurboID. Nat Biotechnol. 2018 Oct;36(9):880-887. doi: 10.1038/nbt.4201. Epub 2018 Aug 20. Erratum in: Nat Biotechnol. 2020 Jan;38(1):108. PMID: 30125270; PMCID: PMC6126969.
Proper citation: RRID:Addgene_173815 Copy
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