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Species: Synthetic
Genetic Insert: luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, Luciferase, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Renilla luciferase with an intron for transient and stable expression in plants. Codon optimized for plants.
Proper citation: RRID:Addgene_123181 Copy
Species: Synthetic
Genetic Insert: Tomato yellow leaf curl virus (TYLCV) V2
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
Proper citation: RRID:Addgene_123183 Copy
Species: Synthetic
Genetic Insert: 2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5874; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC expression vector for plants; Intron avoids expression in Agrobacteria; Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123188 Copy
Species: Synthetic
Genetic Insert: Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, Luciferase, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, firefly luciferase with an intron for transient and stable expression in plants.
Proper citation: RRID:Addgene_123180 Copy
Species: Synthetic
Genetic Insert: J105-30arsR-t-ParsR-30gfp-t
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123162 Copy
Species: Synthetic
Genetic Insert: J115-30arsR-t-ParsR-30gfp-t
Vector Backbone Description: Backbone Size:3339; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123161 Copy
Species: Synthetic
Genetic Insert: J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
Vector Backbone Description: Backbone Size:3339; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123151 Copy
Species: Synthetic
Genetic Insert: J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
Vector Backbone Description: Backbone Size:3339; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123150 Copy
Species: Synthetic
Genetic Insert: J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
Vector Backbone Description: Backbone Size:3339; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123158 Copy
Species: Synthetic
Genetic Insert: J117-30arsR-t-ParsR-30gfp-t
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123142 Copy
Species: Synthetic
Genetic Insert: J109-32merR-t-PmerT-30gfp-t
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123148 Copy
Species: Synthetic
Genetic Insert: Red-enhanced Nanolantern
Vector Backbone Description: Backbone Size:5572; Vector Backbone:pCAGGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650390
Proper citation: RRID:Addgene_123138 Copy
Species: Synthetic
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Size:9004; Vector Backbone:pRG01; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962622
Proper citation: RRID:Addgene_123363 Copy
Species: Synthetic
Genetic Insert: cp-moxCerulean3-moxCerulean3-FLARE-D1ER
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123354 Copy
Species: Synthetic
Genetic Insert: human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
Vector Backbone Description: Backbone Size:10130; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962622
Proper citation: RRID:Addgene_123359 Copy
Species: Synthetic
Genetic Insert: Venus-cpVenus-FLARE-EKAR-EV(T/A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123340 Copy
Species: Synthetic
Genetic Insert: ArchT-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4763; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_123607 Copy
Species: Synthetic
Genetic Insert: FLARE16-S aadA-GFP
Vector Backbone Description: Vector Backbone:pMK-T; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. This insert is a coding sequence designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123562 Copy
Species: Synthetic
Genetic Insert: crRNA scaffold 4n96
Vector Backbone Description: Vector Backbone:pUC19, BsaI site mutated; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123640 Copy
Species: Synthetic
Genetic Insert: AnnexinA5
Vector Backbone Description: Vector Backbone:pDEST-Tol2-pA2 a-cry-mCherry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_123628 Copy
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