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Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Please note that Addgene was not able to completely confirm the truncation with quality control sequencing.
Proper citation: RRID:Addgene_21247 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Proper citation: RRID:Addgene_21245 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21240 Copy
Species: Mus musculus
Genetic Insert: TNF receptor-associated factor 6
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15456887
Proper citation: RRID:Addgene_21624 Copy
Species: Mus musculus
Genetic Insert: GFP-ING2(pHD)
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12859901
Proper citation: RRID:Addgene_21589 Copy
Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Proper citation: RRID:Addgene_21834 Copy
Species: Mus musculus
Genetic Insert: XBP-1 processed/spliced
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V.
FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.
Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.
Proper citation: RRID:Addgene_21833 Copy
Species: Mus musculus
Genetic Insert: XBP-1 unprocessed
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V.
FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.
Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.
Proper citation: RRID:Addgene_21832 Copy
Species: Mus musculus
Genetic Insert: Pten
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21952 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:1547942
Comments: Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21899 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4310; Vector Backbone:pGL3 and pGL2 modified; Vector Types:Non-viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Comments: This reporter gene closely tracks the expression of endogenous CHOP.
Proper citation: RRID:Addgene_21898 Copy
Species: Mus musculus
Genetic Insert: PERK (C-term deleted)
Vector Backbone Description: Backbone Size:4756; Vector Backbone:pCDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9930704
Comments: Mouse PERK, C-term deleted (removes amino-acids 582-1081), 9E10-tagged at C-terminus, mammalian expression vector.
Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.
Proper citation: RRID:Addgene_21816 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: From depositing lab:
"9E10 (Myc-epitope) tagged mouse CHOP coding region only. The insert was joined to the epitope tag using patch-PCR and incorporating unique BamH1 and Xho1 sites for ligation into pcDNA1. Stocks are provided as MC1060 (or MC1061, we are not sure) host strain transformed with the plasmid. Note that pCDNA1 plasmids must be propagated on combined amp and tet selection. I would recommedn that you consider transferring the insert into a better host strain such as pcDNA3"
Proper citation: RRID:Addgene_21913 Copy
Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Addgene sequencing results show an additional mutation in the coding region, causing L850P substitution in the amino acid sequence.
Mouse IRE1b, K536A, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3. The K->A mutation introduces a second MSc1 site into the plasmid at position 2578.
Proper citation: RRID:Addgene_21881 Copy
Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Mouse IRE1b, wild-type, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3.
Proper citation: RRID:Addgene_21880 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)
Proper citation: RRID:Addgene_21857 Copy
Species: Mus musculus
Genetic Insert: mCD36
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)
Proper citation: RRID:Addgene_21853 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)
Proper citation: RRID:Addgene_21852 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11106749
Comments: Addgene sequencing results show 2 mismatches with author's sequence and NCBI sequence for ATF4.
Mouse ATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter in a pGL3 backbone
TK.mATF4.UTR.Luc.pGL3 (5202nt, predicted). TK promoter: 58..184; mATF4 5'UTR and AUG: 185..470; luciferase coding sequence: 472..2121; poly A signals: 2156..2377
Proper citation: RRID:Addgene_21850 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Size:4767; Vector Backbone:pcDNA1.1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: 9E10 (Myc-epitope) tagged mouse CHOP coding region only, S78A and S81A double mutant. The S78A and S81A mutation introduces an additional HhaI site not present in the wildtype insert. Note that there is considerable uncertainity regarding this plasmid.
Proper citation: RRID:Addgene_21902 Copy
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