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Species: Homo sapiens
Genetic Insert: NFkB p65 subunit
Vector Backbone Description: Backbone Marker:Matthias P et al. Nucleic Acids Research (1989). 17(15): 6418; Backbone Size:5120; Vector Backbone:pEv3s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11533489
Proper citation: RRID:Addgene_21984 Copy
Species: Mus musculus
Genetic Insert: Sprouty 2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10498682
Comments: mSprouty cDNA clone was isolated from a mouse E11 cDNA library (Clonetech). The entire coding region as well as 5' and 3' UTR sequences are included.
For antisense probe, linearize the plasmid with SacII and use T3 Polymerase. The transcript should be approximately 1.5kb.
Proper citation: RRID:Addgene_22097 Copy
Genetic Insert: synthethic oligonucleotide
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:7394; Vector Backbone:pSUPER_Retro-GFP/Neo; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17526729
Comments: shRNA oligo sequence - 5'-GATCCCCGACAAGGTCTGCAAAGGGTTTCAAGAGAACCCTTTGCAGACCTTGTCTTTTT
Proper citation: RRID:Addgene_22131 Copy
Genetic Insert: synthethic oligonucleotide
Vector Backbone Description: Backbone Marker:NKI-AVL; Backbone Size:6349; Vector Backbone:pRetro-Super; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14691554
Proper citation: RRID:Addgene_22100 Copy
Genetic Insert: synthethic oligonucleotide
Vector Backbone Description: Backbone Marker:NKI-AVL; Backbone Size:6349; Vector Backbone:pRetro-Super; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14691554
Proper citation: RRID:Addgene_22101 Copy
Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599482
Proper citation: RRID:Addgene_22117 Copy
Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599482
Proper citation: RRID:Addgene_22113 Copy
Species: Homo sapiens
Genetic Insert: SA-Puro
Vector Backbone Description: Backbone Size:5890; Vector Backbone:N/A; Vector Types:ZFN donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19680244
Comments: Please note that this plasmid contains a non-functional ccdB gene. The use of ccdB survival cells may be needed when subcloning or modifying this vector if the number of transformants is low.
Proper citation: RRID:Addgene_22075 Copy
Species: C. reinhardtii
Genetic Insert: ChR2-GFP
Vector Backbone Description: Backbone Marker:Lois/Baltimore; Backbone Size:9115; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16116447
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_22051 Copy
Species: Herpes Simplex Type 1
Genetic Insert: HSV Type 1 thymidine kinase
Vector Backbone Description: Backbone Size:8294; Vector Backbone:pAL120; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18287240
Proper citation: RRID:Addgene_22053 Copy
Species: Homo sapiens
Genetic Insert: partial sequence of human albumine
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22037 Copy
Species: HIV-1
Genetic Insert: gag pol tat rev
Vector Backbone Description: Backbone Size:4661; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22036 Copy
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10854432
Comments: The internal restriction sites may not be correct
Proper citation: RRID:Addgene_22152 Copy
Species: Homo sapiens
Genetic Insert: Epsin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10567358
Proper citation: RRID:Addgene_22242 Copy
Species: Homo sapiens
Genetic Insert: vexGFP_2A_OCT4
Vector Backbone Description: Backbone Size:6655; Vector Backbone:N/A; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19549847
Proper citation: RRID:Addgene_22240 Copy
Species: Homo sapiens
Genetic Insert: HRAS G12V
Vector Backbone Description: Backbone Size:8378; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22259 Copy
Species: Rattus norvegicus
Genetic Insert: Genetic suppressor element #22, T329A
Vector Backbone Description: Backbone Size:8712; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Encodes amino acids #312-391 of rat p53 to act as a dominant-negative
(Ossovskaya et al. 1996, PNAS 93:10309-10314).
T329A mutation does not prevent tetramerization of p53
(Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).
Proper citation: RRID:Addgene_22255 Copy
Species: Homo sapiens
Genetic Insert: HRAS G12V
Vector Backbone Description: Backbone Size:2466; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_22252 Copy
Species: Rattus norvegicus
Genetic Insert: Epsin 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9723620
Comments: Please note: Addgene's quality control sequence found the EPSIN1 gene has an insertion of threonine after aa 821 and since there is no stop codon, ESPIN1 contains the additional c terminal residues VDGTAGPGSTGSR. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_22228 Copy
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8524249
Comments: This vector was the backbone for several of the Ananda Roy plasmids.
SpeI and KpnI site are missing from the MCS, unlike the original Baltimore vector.
Proper citation: RRID:Addgene_22226 Copy
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