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Species: Synthetic
Genetic Insert: Cas9-VirD2
Vector Backbone Description: Vector Backbone:pRGEB32; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31974493
Proper citation: RRID:Addgene_139802 Copy
Species: Homo sapiens
Genetic Insert: TBXT, DNA-binding domain, G177D
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:5424; Vector Backbone:pNIC-Bio3 (Genbank: JN792439.1); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: E41- D225: Codon-optimized for E. coli expression
Proper citation: RRID:Addgene_139767 Copy
Species: Lactate oxidase from A. viridans and Catalase from E. coli
Genetic Insert: A fusion of lactate oxidase from A. viridans and catalase from E. coli
Vector Backbone Description: Backbone Size:8880; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932725
Proper citation: RRID:Addgene_139971 Copy
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31942010
Proper citation: RRID:Addgene_139977 Copy
Genetic Insert: barcode P1 and sgRNA lacO1 array
Vector Backbone Description: Vector Backbone:pGuide; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31740817
Proper citation: RRID:Addgene_140040 Copy
Species: Synthetic
Genetic Insert: pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Vector Backbone Description: Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_140082 Copy
Species: Oryza sativa
Genetic Insert: ORF1 Shuffle 1 NLS
Vector Backbone Description: Backbone Marker:CSIRO; Vector Backbone:pCambia; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33506165
Comments: The plasmid contains an IS element 5' of the KanR marker that is not currently of functional concern. Confirm IS element remains in expected position prior to use or when troubleshooting.
Proper citation: RRID:Addgene_140006 Copy
Genetic Insert: lacY
Vector Backbone Description: Backbone Size:4938; Vector Backbone:pAN1818; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32355194
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_140141 Copy
Species: Synthetic
Genetic Insert: pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pEarleyGate100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140169 Copy
Species: Synthetic
Genetic Insert: pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Vector Backbone Description: Vector Backbone:pEarleyGate100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33112725
Proper citation: RRID:Addgene_140170 Copy
Species: Lactate oxidase from A. viridans and Catalase from E. coli
Genetic Insert: A fusion of lactate oxidase from A. viridans and catalase from E. coli
Vector Backbone Description: Backbone Size:8838; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932725
Proper citation: RRID:Addgene_140085 Copy
Species: Lactate oxidase from A. viridans and catalase from E. coli
Genetic Insert: A fusion of lactate oxidase from A. viridans and catalase from E. coli
Vector Backbone Description: Backbone Size:8838; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932725
Proper citation: RRID:Addgene_140084 Copy
Species: Sinomenium acutum
Genetic Insert: Dechloroacutumine halogenase from Sinomenium acutum
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes chlorination of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140128 Copy
Species: Menispermum canadense
Genetic Insert: Dechloroacutumine halogenase from Menispermum canadense
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes chlorination of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140129 Copy
Species: Sinomenium acutum
Genetic Insert: Dechloroacutumine halogenase G226D variant from Sinomenium acutum
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes hydroxylation of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140130 Copy
Genetic Insert: lacY
Vector Backbone Description: Backbone Size:4938; Vector Backbone:pAN1818; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32355194
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_140139 Copy
Species: Synthetic
Genetic Insert: Stabilized hairpin of miR-let7d
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: let7d_stbC motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140266 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of mutant MS2CP (MS2CP(V29I)) was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140260 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140278 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140288 Copy
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