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Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Addgene #25890; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of Luciferase with C-terminal V5 tag.
Proper citation: RRID:Addgene_98580 Copy
Species: Other
Genetic Insert: MS2
Vector Backbone Description: Backbone Size:3100; Vector Backbone:Derived from pDMSD-Y2 (Kostecki et al., 2010, 10.1371/journal.pone.0009225); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25080893
Proper citation: RRID:Addgene_98587 Copy
Species: Other
Genetic Insert: shLacZ
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the TRC (TRCN0000072238). Used as control knockdown vector.
Proper citation: RRID:Addgene_98632 Copy
Species: Other
Genetic Insert: mEos3.2-A69T
Vector Backbone Description: Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Proper citation: RRID:Addgene_98571 Copy
Species: Other
Genetic Insert: mKikGR-V69T
Vector Backbone Description: Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Proper citation: RRID:Addgene_98574 Copy
Species: Other
Genetic Insert: pcDronpa2-A69T
Vector Backbone Description: Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Proper citation: RRID:Addgene_98578 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374 (Addgene 59896); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98698 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Addgene #25896; Backbone Size:9377; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of LacZ with C-terminal V5 tag.
Proper citation: RRID:Addgene_98579 Copy
Species: Other
Genetic Insert: NS1
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28323816
Proper citation: RRID:Addgene_99022 Copy
Species: Other
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99113 Copy
Species: Other
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99114 Copy
Species: Other
Genetic Insert: DIO-tdTomato
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: Prone to recombination even in stable cell lines
Proper citation: RRID:Addgene_99116 Copy
Species: Other
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: *Note: The tTA in this plasmid was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).
Proper citation: RRID:Addgene_99119 Copy
Species: Other
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: *Note: The tTA in this plasmid was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).
Proper citation: RRID:Addgene_99120 Copy
Species: Other
Genetic Insert: DIO-tTA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: *Note: The tTA in this plasmid was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).
Proper citation: RRID:Addgene_99121 Copy
Species: Other
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99123 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: The left ITR in this construct contains a 22bp deletion. We have not observed this change to impact vector production or function.
Proper citation: RRID:Addgene_99128 Copy
Species: Other
Genetic Insert: mKate2.5f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: The depositing laboratory has confirmed that sequence discrepancies between the Addgene NGS Result and the provided pAAV-GFAP-mKate2.5f.gb do not affect plasmid function. The left ITR in this construct contains a 22bp deletion. We have not observed this change to impact vector production or function.
Proper citation: RRID:Addgene_99129 Copy
Species: Other
Genetic Insert: HA-H2B-Cerulean
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28802047
Proper citation: RRID:Addgene_99618 Copy
Species: Other
Genetic Insert: Mb2EYFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28802047
Proper citation: RRID:Addgene_99619 Copy
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