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Species: Other
Genetic Insert: boule2
Vector Backbone Description: Vector Backbone:pJC53.2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27330085
Comments: Addgene Note: These plasmids were generated for in vitro transcription and may not be suitable for overexpression studies. The inserts were amplified from cDNA and may differ from NCBI reference sequences.
Proper citation: RRID:Addgene_99762 Copy
Species: Other
Genetic Insert: boule1
Vector Backbone Description: Vector Backbone:pJC53.2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27330085
Comments: Addgene Note: These plasmids were generated for in vitro transcription and may not be suitable for overexpression studies. The inserts were amplified from cDNA and may differ from NCBI reference sequences.
Proper citation: RRID:Addgene_99761 Copy
Species: Other
Genetic Insert: CDC25-1
Vector Backbone Description: Vector Backbone:pJC53.2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27330085
Comments: Addgene Note: These plasmids were generated for in vitro transcription and may not be suitable for overexpression studies. The inserts were amplified from cDNA and may differ from NCBI reference sequences.
Proper citation: RRID:Addgene_99763 Copy
Species: Other
Genetic Insert: DAZAP1
Vector Backbone Description: Vector Backbone:pJC53.2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27330085
Comments: Addgene Note: These plasmids were generated for in vitro transcription and may not be suitable for overexpression studies. The inserts were amplified from cDNA and may differ from NCBI reference sequences.
Proper citation: RRID:Addgene_99766 Copy
Species: Other
Genetic Insert: CDC25-3
Vector Backbone Description: Vector Backbone:pJC53.2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27330085
Comments: Addgene Note: These plasmids were generated for in vitro transcription and may not be suitable for overexpression studies. The inserts were amplified from cDNA and may differ from NCBI reference sequences.
Proper citation: RRID:Addgene_99765 Copy
Species: Other
Genetic Insert: anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Backbone Size:5300; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35314489
Comments: Vectors are based on Buser et al., 2018 (PNAS), and Buser and Spiess, 2019 (JoVE) (https://www.addgene.org/Martin_Spiess/). The anti-mCherry nanobody sequence (LaM4) is based on Fridy et al., 2014 (Nature Methods)
Proper citation: RRID:Addgene_182641 Copy
Species: Other
Genetic Insert: hM4D(Gi)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183704 Copy
Species: Other
Genetic Insert: rtTA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183707 Copy
Species: Other
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Expresses tTA and a E2-Crimson; separated by 2A domain
Proper citation: RRID:Addgene_183708 Copy
Species: Other
Genetic Insert: hM3D(Gq)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183705 Copy
Species: Other
Genetic Insert: PolyA-HhR
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148303 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pLKO5d; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28794406
Proper citation: RRID:Addgene_90333 Copy
Species: Other
Genetic Insert: TetR
Vector Backbone Description: Vector Backbone:pExchange-tdk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28431252
Comments: Addgene's sequencing results found multiple mutations in ermG. The depositing lab confirmed that these mutations do not compromise erythromycin selection.
Proper citation: RRID:Addgene_90327 Copy
Species: Other
Genetic Insert: TetR
Vector Backbone Description: Vector Backbone:pExchange-tdk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28431252
Comments: Addgene's sequencing results found multiple mutations in ermG. The depositing lab confirmed that these mutations do not compromise erythromycin selection.
Proper citation: RRID:Addgene_90326 Copy
Species: Other
Genetic Insert: TetR
Vector Backbone Description: Vector Backbone:pExchange-tdk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28431252
Comments: Addgene's sequencing results found multiple mutations in ermG. The depositing lab confirmed that these mutations do not compromise erythromycin selection.
Proper citation: RRID:Addgene_90325 Copy
Species: Other
Genetic Insert: cyto-sFD, cyto-sFNR
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301067
Proper citation: RRID:Addgene_90510 Copy
Species: Other
Genetic Insert: mito-sHO1, mito-Hy2
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301067
Proper citation: RRID:Addgene_90509 Copy
Species: Other
Genetic Insert: mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301067
Proper citation: RRID:Addgene_90502 Copy
Species: Other
Genetic Insert: mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301067
Proper citation: RRID:Addgene_90504 Copy
Species: Other
Genetic Insert: mito-tFD and mito-tFNR
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301067
Comments: Additional growth instructions: For larger cultures, the depositing lab minipreps a sample before midiprepping. Perhaps if you are just making glycerol stocks try growing 2 or maybe 3 large cultures and miniprepping a sample to check for the full sized plasmid. They also try to start with a small sized colony and are more stringent about not letting the culture overgrow.
Proper citation: RRID:Addgene_90499 Copy
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