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Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:Hy_pMtnA eGFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31530651
Proper citation: RRID:Addgene_132650 Copy
Species: Danio rerio
Genetic Insert: gata2a endothelial enhancer (x6) with a b-actin basal promoter
Vector Backbone Description: Backbone Size:2643; Vector Backbone:pDONR221; Vector Types:Gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31564611
Proper citation: RRID:Addgene_132968 Copy
Species: MLV
Genetic Insert: N-tropic MLV gag-pol
Vector Backbone Description: Vector Backbone:pCIG3 N; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31636416
Proper citation: RRID:Addgene_132941 Copy
Species: Rattus norvegicus
Genetic Insert: 14-3-3 epsilon
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428519
Comments: Insert from epsilon-MBP cloned into BamHI and SmaI (which is now lost).
Proper citation: RRID:Addgene_13279 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 eta
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428519
Comments: Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
Proper citation: RRID:Addgene_13277 Copy
Species: Bos taurus
Genetic Insert: 14-3-3 zeta
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428519
Comments: Insert PCR'd and cloned into pGEX-4T1 (via either EcoRI and/or BamHI?) by Tom Roberts lab.
Uses stop codons of pGEX backbone rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
Proper citation: RRID:Addgene_13278 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 beta
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428519
Comments: Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
Proper citation: RRID:Addgene_13276 Copy
Species: Synthetic
Genetic Insert: HEK3_CTT_ins pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31634902
Comments: Insert size: 122 bp.
Proper citation: RRID:Addgene_132778 Copy
Species: HIV
Genetic Insert: gag and pol
Vector Backbone Description: Vector Backbone:p8.9 NdeltaSB; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15479815
Proper citation: RRID:Addgene_132929 Copy
Species: Rattus norvegicus
Genetic Insert: 14-3-3 gamma
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10488331
Proper citation: RRID:Addgene_13280 Copy
Species: Synthetic
Genetic Insert: sgFah
Vector Backbone Description: Vector Backbone:pAAVsc; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30102296
Proper citation: RRID:Addgene_121508 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter PH2 domain
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12488434
Proper citation: RRID:Addgene_12163 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:6943; Vector Backbone:pLS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9733856
Comments: pLS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.
Proper citation: RRID:Addgene_12161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsc82
Vector Backbone Description: Backbone Size:13200; Vector Backbone:YEp24; Vector Types:Yeast Expression, 2 micron; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7791797
Proper citation: RRID:Addgene_1215 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:7088; Vector Backbone:pCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9733856
Comments: pCS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.
Proper citation: RRID:Addgene_12154 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged hM4D(Gi)
Vector Backbone Description: Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31063250
Proper citation: RRID:Addgene_121538 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged hM3D(Gq)
Vector Backbone Description: Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31063250
Proper citation: RRID:Addgene_121539 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121492 Copy
Species: Cavia porcellus (guinea pig)
Genetic Insert: gpASNase1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6813; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29941931
Proper citation: RRID:Addgene_121526 Copy
Species: Mus musculus
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pGIPz; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121488 Copy
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