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Genetic Insert: Auxin-inducible degron (AID) and mCherry
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34213414
Comments: AID, T2A and mCherry are flanked by homology arms of human PABPC1 genomic regions near the stop codon. Used with C119 and the PAM region is mutated.
Proper citation: RRID:Addgene_174355 Copy
Genetic Insert: OsTIR1
Vector Backbone Description: Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34213414
Proper citation: RRID:Addgene_174356 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GALL promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Size:2574; Vector Backbone:p404ADH1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17418 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET3 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Size:2581; Vector Backbone:p404ADH1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17419 Copy
Species: Xenopus laevis
Genetic Insert: PABPC1 (M161A)
Vector Backbone Description: Vector Backbone:pGEM_11Zf; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34213414
Comments: The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length.
Proper citation: RRID:Addgene_174349 Copy
Genetic Insert: IL6ST cytosolic domain and sarA chimera
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174395 Copy
Genetic Insert: pyrF-CS
Vector Backbone Description: Vector Backbone:pBM23; Vector Types:Bacterial Expression, Template for cloning fragments for chromosomal integration into Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174396 Copy
Species: Candida auris
Genetic Insert: C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
Vector Backbone Description: Backbone Size:2236; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174432 Copy
Species: Candida auris
Genetic Insert: HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174433 Copy
Species: Synthetic
Genetic Insert: Candida optimized monomeric GFP
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174434 Copy
Species: Other
Genetic Insert: EPSUD
Vector Backbone Description: Vector Backbone:pHFX-B60; Vector Types:archaeal gene knockout vector for Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174390 Copy
Species: Other
Genetic Insert: a crRNA targeting the template strand
Vector Backbone Description: Backbone Size:7876; Vector Backbone:pWL502; Vector Types:Bacterial Expression, CRISPR, archaeal expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174383 Copy
Genetic Insert: pyrF-NT
Vector Backbone Description: Vector Backbone:pBM23; Vector Types:Bacterial Expression, Template for cloning fragments for chromosomal integration into Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174384 Copy
Genetic Insert: pyrF
Vector Backbone Description: Vector Backbone:pMD18; Vector Types:Bacterial Expression, Template for cloning fragments for chromosomal integration into Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174388 Copy
Species: Drosophila melanogaster
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531964
Proper citation: RRID:Addgene_174421 Copy
Species: Other
Genetic Insert: PAM-spacer
Vector Backbone Description: Backbone Size:7876; Vector Backbone:pWL502; Vector Types:Bacterial Expression, CRISPR, archaeal expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174382 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:2691; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17437 Copy
Species: Rhabdomys pumilio
Genetic Insert: Centromere protein C
Vector Backbone Description: Vector Backbone:pIVT; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433012
Proper citation: RRID:Addgene_174446 Copy
Species: Mus musculus
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCS2+ NLSMT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003914
Comments: Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
Proper citation: RRID:Addgene_17440 Copy
Vector Backbone Description: Backbone Marker:Philippa Marrack Lab; Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Comments: Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.
Proper citation: RRID:Addgene_17442 Copy
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