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Species: Other
Genetic Insert: Tf_dr2_ph90_01_b.46_h99_dd97_5
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257216 Copy
Species: Other
Genetic Insert: Tf_dr2_1219_ICCG_b.46_h99_dd97_4
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257213 Copy
Species: Other
Genetic Insert: Tf_dr3_1159ICCG5_b.45_h99_dd97_8
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257222 Copy
Species: Other
Genetic Insert: Tf_dr2_1145_ICCG_b.45_h99_dd97_7
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257219 Copy
Species: Other
Genetic Insert: Tf_dr3_1219ICCG1_b.46_h99_dd97_6
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257217 Copy
Species: Other
Genetic Insert: Tf_dr3_1219ICCG4_b.46_h99_dd97_6
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257218 Copy
Species: Other
Genetic Insert: J23119 CAO1 target + CAO1 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253627 Copy
Species: Other
Genetic Insert: J23119 GFP T14 target + GFP T14 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253631 Copy
Species: Other
Genetic Insert: J23119 GFP T14 target + CAO1 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253632 Copy
Species: Other
Genetic Insert: IS1_TS1-A-PgpdA-uidA-TtrpC-B-IS1_TS2-ori-AmpR
Vector Backbone Description: Vector Backbone:pU2002; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.09.13.612835 for bioRxiv preprint.
Proper citation: RRID:Addgene_247963 Copy
Species: Other
Genetic Insert: Geo Sec Tag
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235442 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235444 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235446 Copy
Species: Other
Genetic Insert: Fncpf1
Vector Backbone Description: Vector Backbone:pFW2000; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34995484
Proper citation: RRID:Addgene_224215 Copy
Species: Other
Genetic Insert: His6-GG-UbK48R
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226136 Copy
Species: Other
Genetic Insert: MC-Ub-SH3-3C-His6
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226134 Copy
Species: Other
Genetic Insert: TurboID-FRB
Vector Backbone Description: Vector Backbone:pFa6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228022 Copy
Species: Other
Genetic Insert: Luciferase and hRluc
Vector Backbone Description: Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32168374
Comments: This plasmid contains an IS4 sequence. The depositor has confirmed this is not of concern to plasmid function.
Proper citation: RRID:Addgene_226869 Copy
Species: Other
Genetic Insert: anti-SARS-CoV-2 Nucleocapsid nanobody ON10
Vector Backbone Description: Backbone Size:6220; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37497229
Proper citation: RRID:Addgene_198691 Copy
Species: Other
Genetic Insert: FAST-PETase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39582006
Proper citation: RRID:Addgene_225760 Copy
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