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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pC-GoldyTALEN Resource Report Resource Website 10+ mentions |
RRID:Addgene_38143 | GoldyTALEN | Synthetic | Ampicillin | PMID:23027955 | For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899). For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson | Backbone Size:4000; Vector Backbone:pBSK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Truncate at N and C terminous | 2026-08-15 01:14:19 | 12 |
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p1177-X1-8 Resource Report Resource Website |
RRID:Addgene_38150 | Carboxypeptidase A | M. anisopliae | Ampicillin | PMID:21073956 | Backbone Marker:Invitrogen; Backbone Size:6526; Vector Backbone:pFastBac-1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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pCW57.1-4EBP1_4xAla Resource Report Resource Website 10+ mentions |
RRID:Addgene_38240 | 4EBP1 | Rattus norvegicus | Ampicillin | PMID:22552098 | Backbone Marker:Broad Insitute; Backbone Size:5000; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin | T37A, T46A, S65A, T70A (dominant negative) | 2026-08-15 01:14:20 | 15 | |
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pGE-mSmo-YFP Resource Report Resource Website |
RRID:Addgene_38094 | Smo | Mus musculus | Kanamycin | PMID:12414725 | Vector Backbone:pGE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:19 | 0 | ||
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pMXs-IP-EGFP-LC3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38195 | microtubule-associated protein 1 light chain 3 beta | Rattus norvegicus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pMXs-IP-EGFP-ULK1(K46N) Resource Report Resource Website 1+ mentions |
RRID:Addgene_38197 | a novel protein kinase structurally related to C. elegans UNC-51 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | changed Lysine 46 to Asparagine | 2026-08-15 01:14:20 | 2 | |
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pMXs-IP-EGFP-ULK1(1-828) Resource Report Resource Website |
RRID:Addgene_38198 | a novel protein kinase structurally related to C. elegans UNC-51 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | deleted amino acids 829-1051 | 2026-08-15 01:14:20 | 0 | |
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pTH736-CEN-staHIS3 Resource Report Resource Website |
RRID:Addgene_38228 | HIS3 | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 0 | ||
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pTH743-CEN-RLuc/slowstaCFLuc Resource Report Resource Website |
RRID:Addgene_38223 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
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human CRKL transgenic construct Resource Report Resource Website |
RRID:Addgene_38186 | CRKL | Homo sapiens | Ampicillin | PMID:11245441 | This construct was made by Arnoud van Wijk. It can be used to make transgenic mice. The DNA construct contains 7.5 kb of human CRKL locus 5’ sequences including the promoter of the human v-crk sarcoma virus CT10 oncogene homolog (CRKL) gene, joined to CRKL exon 1. Part of the intron between exons 1 and 2 encompassing around 8-kb is included and exons 2-3 as cDNA. The entire insert is around 17 kb and can be isolated free from plasmid sequences in a Kpn I x Mlu I digestion. Jackson labs stock no. 017834 is one of our CRKL transgenics containing approximately 5 copies of this transgene. CRKL overexpressing transgenics are viable but mice with high copy numbers have reduced fertility. How the CRKL transgenic construct was made: (verbatim from PMID 11245441): Human genomic clone 70 (PMID 7905853) contains 12 kb of 5’ sequences, exon 1, and 6.6 kb of intron 1 of the CRKL gene. A 350-bp RsaI fragment isolated from a CRKL cDNA (GenBank accession no. X59656) was used as a probe to specifically isolate a phage clone, CR-4, containing CRKL exon 2 flanked by 6.5 kb of intron 1 and 9.5 kb of intron 2. CRKL is located on human chromosome 22 and has been entirely sequenced; introns 1 and 2 are both around 15.5 kb. To generate a transgenic DNA construct, a 7-kb SalI-BamHI fragment from CR-4, which included a SalI site at the 5’ end from the phage polylinker and the 3’ BamHI site located in exon 2, was ligated with a 0.78-kb BamHI-EcoRI cDNA fragment, including exons 2, 3, and the 3’ untranslated region, into pSK digested with SalIxEcoRI. The insert was removed as a 7.7-kb SalI-NotI fragment and ligated with a 1.2-kb SstII-SalI fragment from intron 1 in clone 70 into pSK digested with SstII x NotI. The resulting insert was removed by digestion with SstII x NotI. The 5’ promoter and exon 1 sequences were isolated on an 8-kb EcoRI-SstII fragment that was subcloned into pSK digested with EcoRI x SstII; the insert was removed as an 8-kb KpnI-SstII fragment. The 8-kb KpnI-SstII fragment plus the 7.7-kb SstII-NotI fragment were ligated into pSL1180 digested with KpnI x NotI. | Backbone Size:3500; Vector Backbone:pSL1180; Vector Types:mouse transgenic; Bacterial Resistance:Ampicillin | Contains human CRKL introns | 2026-08-15 01:14:20 | 0 |
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pTH738-CENBEVYslow Resource Report Resource Website |
RRID:Addgene_38220 | GCN4-based single uORF-containing 5'-UTR | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The start codons of uORFs2, 3 and4 of the yeast Gcn4 leader sequence were mutated to non-start codons. | 2026-08-15 01:14:20 | 0 | |
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pMXs-IP-EGFP-hAtg13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38191 | autophagy-related genes 13 | Homo sapiens | Ampicillin | PMID:19211835 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pMXs-puro-EGFP-FAK Resource Report Resource Website 1+ mentions |
RRID:Addgene_38194 | PTK2 protein tyrosine kinase 2 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 3 | ||
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pTH753-CEN-RLuc/min346maxCFLuc Resource Report Resource Website |
RRID:Addgene_38219 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The first 346 codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. All remaining codons were exchanged for those isoaccepting codons decoded by the most abundant tRNAs. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
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pTH751-CEN-RLuc/min53maxCFLuc Resource Report Resource Website |
RRID:Addgene_38217 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The first 53 codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. All remaining codons were exchanged for those isoaccepting codons decoded by the most abundant tRNAs. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
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pTH747-CEN-RLuc/min4maxCFLuc Resource Report Resource Website |
RRID:Addgene_38213 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The first four codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. All remaining codons were exchanged for those isoaccepting codons decoded by the most abundant tRNAs. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
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pCDNA3.1Xpress-BcrGAP R1090A Resource Report Resource Website |
RRID:Addgene_38176 | BCR GAP domain encoding residues 1004-1271 with R1090A point mutation | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. pcDNA3.1Xpress-BcrGAP plasmid(AddGene #38175) was digested by BstEII x EcoRI and a 1.2 kb fragment containing the R1090 wt site, TAG and 3’ UT sequences removed. The wt fragment was replaced by a 0.6 kb BstEII x EcoRI fragment containing the R1090A mutation isolated from EGFP-BCR R1090A pEGFP (AddGene #38173). The construct contains the natural TAG and 3’ UT sequences. The difference between wt and mutant plasmids is in the 3’ UT region. The construct includes amino acid residues 1004-1271. | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa 1004-1271 with R1090A point mutation | 2026-08-15 01:14:19 | 0 |
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Abr DH domain NE/AA pGEX Resource Report Resource Website |
RRID:Addgene_38182 | ABR DH domain N282AE283A | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. Mutagenesis done using Stratagene Quick Change kit. 0.35 kb Bgl IIxSstI fragment containing the N282A/E283A double mutation from Abr EYFP (AddGene #36419) was ligated into the "ABR DH domain pGEX" plasmid (AddGene #38181) digested with Bgl II x SstI and removal of the 0.35 kb fragment containing the wt sequence N282E283. The amino acid numbering is as in NM_021962 (isoform A). | Backbone Size:5000; Vector Backbone:pGEX 3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | ABR DH domain with N282A and E283A mutations | 2026-08-15 01:14:20 | 0 |
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pBad-Xis/Int Reset Flipper Resource Report Resource Website 1+ mentions |
RRID:Addgene_38207 | Bxb1-Excisionase | Chloramphenicol | PMID:22615351 | In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate and increase the excisionase to integrase ratio. This construct was cloned in a plasmid with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance). | Backbone Size:2800; Vector Backbone:J64100; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:14:20 | 1 | ||
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pBad-Int Set Flipper Resource Report Resource Website |
RRID:Addgene_38209 | Bxb1 Integrase | Kanamycin | PMID:22615351 | The Bxb1 integrase was cloned downstream of the PBAD/AraC promoter (BBa_I0500) on pSB3K1 plasmid bearing a p15A origin of replication (15-20 copies). This version of the integrase has a 6-His-tag which we found to stabilize the protein. Therefore, a weak RBS (BBa_B0031) and a LAA ssrA tag was added to reduce the basal expression of the enzyme. | Vector Backbone:pSB3K1; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:20 | 0 |
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