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Species: Synthetic
Genetic Insert: Pveg-sLucopt
Vector Backbone Description: Backbone Marker:Robert Fagan and Neil Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that the individual components of this construct (that is, Pveg and sLucopt) are described in Oliveira Paiva AM et al (2016) ACS Synth Biol (DOI: 10.1021/acssynbio.6b00104). During the cloning a mutation occurred that removes the SacI site in between the Pveg and sLucopt genes. The 5' (KpnI) and 3' (BamHI) restriction sites remain.
Proper citation: RRID:Addgene_86554 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28174584
Comments: See Hahn et al., 2017
Proper citation: RRID:Addgene_86556 Copy
Species: Synthetic
Genetic Insert: CANE-LV envelope
Vector Backbone Description: Backbone Size:4821; Vector Backbone:pCASGS/ES; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27974160
Proper citation: RRID:Addgene_86666 Copy
Species: Synthetic
Genetic Insert: MTST6
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2700; Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060124
Proper citation: RRID:Addgene_86603 Copy
Species: Synthetic
Genetic Insert: ER-GCaMP6-210
Vector Backbone Description: Backbone Size:9246; Vector Backbone:FCK(1.3)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28162809
Proper citation: RRID:Addgene_86919 Copy
Species: Synthetic
Genetic Insert: mKO2-hCDT1(30-120)
Vector Backbone Description: Backbone Marker:Verma lab (Salk Institute); Backbone Size:7800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27888217
Proper citation: RRID:Addgene_86849 Copy
Species: Synthetic
Genetic Insert: NS3 1-177
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:5420; Vector Backbone:pETDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27940580
Proper citation: RRID:Addgene_86846 Copy
Species: Synthetic
Genetic Insert: Cas9-venus
Vector Backbone Description: Backbone Size:2770; Vector Backbone:Bluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30912045
Proper citation: RRID:Addgene_86986 Copy
Species: Synthetic
Genetic Insert: PCNA3 fused to phosphite dehydrogenase and P450cam
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86971 Copy
Species: Synthetic
Genetic Insert: PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86969 Copy
Species: Synthetic
Genetic Insert: PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86968 Copy
Species: Synthetic
Genetic Insert: NADPH Alanine Dehydrogenase
Vector Backbone Description: Backbone Marker:Lucigen (Middleton, WI); Backbone Size:1800; Vector Backbone:pSMART-HCKan (AF532107.1); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34600151
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
Proper citation: RRID:Addgene_87172 Copy
Species: Synthetic
Genetic Insert: U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87119 Copy
Species: Synthetic
Genetic Insert: U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87118 Copy
Species: Synthetic
Genetic Insert: U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87116 Copy
Species: Synthetic
Genetic Insert: mcherry
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_87144 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:9926; Vector Backbone:pLenti-CaMKIIa-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87254 Copy
Species: Synthetic
Genetic Insert: YFP-mAID-3HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2925; Vector Backbone:pBluescript SK(+); Vector Types:Toxoplasma expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28465425
Proper citation: RRID:Addgene_87259 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system (promoter control)
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87315 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system "Bottom" transcript
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87310 Copy
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