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Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22615351
Comments: This strain contains Addgene plasmid 38200 chromosomally integrated at the Phi80 (Haldimann et al., J of Bacteriology 2001) site of DH5alphaZ1 strain.
Proper citation: RRID:Addgene_38203 Copy
Species: Mus musculus
Genetic Insert: Unc-51 like kinase 2
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18443221
Proper citation: RRID:Addgene_38206 Copy
Species: Homo sapiens
Genetic Insert: FER
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Leena Haataja. 4-way ligation of BamHI/NsiI + NsiI/KpnI + KpnI/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Fer protein but not from amplified virus stocks. Large-scale yield of protein is relatively low.
Proper citation: RRID:Addgene_38167 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22615351
Comments: This construct has attL and attR sites (LR).
To integrate on your favorite E.coli strain, transform in strain containing the pAH123 plasmid (Haldimann et al., J of Bacteriology 2001). Contains a R6K origin of replication, will only replicate in pir2+ strains.
Proper citation: RRID:Addgene_38200 Copy
Species: Mus musculus
Genetic Insert: Unc-51 like kinase 2
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18443221
Proper citation: RRID:Addgene_38201 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22615351
Comments: This strain contains Addgene plasmid 38170 chromosomally integrated at the Phi80 (Haldimann et al., J of Bacteriology 2001) site of DH5alphaZ1 strain.
Proper citation: RRID:Addgene_38202 Copy
Species: Homo sapiens
Genetic Insert: ABR
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Leena Haataja. 4-way ligation of BamHI/StuI + StuI/BstEII + BstEII/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Abr protein.
Addgene sequencing found an E24G point mutation--this does not alter plasmid function.
Proper citation: RRID:Addgene_38162 Copy
Species: Homo sapiens
Genetic Insert: BCR
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Vesa Kaartinen. Makes Bcr protein. Nucleotide sequence from BamHI cloning site to BCR ATG is:
5' GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG
Proper citation: RRID:Addgene_38163 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Nora Heisterkamp. Rac1 cDNA isolated from K562 cDNA library. 5' BamHI site introduced by PCR. Constructed in 3-way ligation: 0.18 kb 5' BamHI-PvuII + 3' 0.4 kb PvuII-EcoRI into pAcG2T. Makes Rac1 in insect cells.
Proper citation: RRID:Addgene_38164 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' 1770 bp from STU2 ORF
Vector Backbone Description: Backbone Marker:GE healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22993214
Proper citation: RRID:Addgene_38314 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' 918 bp from STU2 ORF
Vector Backbone Description: Backbone Marker:GE healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22993214
Proper citation: RRID:Addgene_38315 Copy
Species: Homo sapiens
Genetic Insert: GLRX2
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2HT9/
Proper citation: RRID:Addgene_38795 Copy
Species: Mus musculus
Genetic Insert: Sequestosome-1
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21220506
Comments: Ichimura et al., JBC 2008 283, 22847
Proper citation: RRID:Addgene_38280 Copy
Species: Mus musculus
Genetic Insert: Sequestosome-1
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21220506
Comments: Ichimura et al., JBC 2008 283, 22847
Proper citation: RRID:Addgene_38281 Copy
Species: Mus musculus
Genetic Insert: Sequestosome-1
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21220506
Comments: Ichimura et al., JBC 2008 283, 22847
Proper citation: RRID:Addgene_38282 Copy
Species: Mus musculus
Genetic Insert: Zinc finger FYVE domain-containing protein 1
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20639694
Proper citation: RRID:Addgene_38269 Copy
Species: MW polyomavirus, strain WD976
Genetic Insert: 2545-3841 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 2545-3841 of GenBank ID JQ898292. The five nucleotide mismatches are at bp# 2545-2548 and 3838 when compared to this reference sequence.
Proper citation: RRID:Addgene_38787 Copy
Species: MW polyomavirus, strain WD976
Genetic Insert: 4385-462 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 4385-4939 and 1-462 (4385-462) of GenBank ID JQ898292.
Proper citation: RRID:Addgene_38788 Copy
Species: Homo sapiens
Genetic Insert: autophagy related genes 14
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18843052
Proper citation: RRID:Addgene_38265 Copy
Species: MW polyomavirus, strain MA095
Genetic Insert: 203-1408 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 203-1408 of GenBank ID JQ898291
Proper citation: RRID:Addgene_38782 Copy
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