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Species: Synthetic
Genetic Insert: N-terminal tag, HiBiT-TrxA-[TEV cleavage site]
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162299 Copy
Species: Synthetic
Genetic Insert: N-terminal tag, TrxA-[TEV cleavage site]
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162298 Copy
Species: Synthetic
Genetic Insert: N-terminal tag, HiBiT
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162291 Copy
Species: Escherichia coli
Genetic Insert: E. coli SRP RNA
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33453165
Comments: Also referenced in: Watters, K., Strobel, E., Yu, A. et al. Cotranscriptional folding of a riboswitch at nucleotide resolution. Nat Struct Mol Biol 23, 1124–1131 (2016). https://doi.org/10.1038/nsmb.3316.
Please visit https://www.biorxiv.org/content/10.1101/379222v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162240 Copy
Species: Synthetic
Genetic Insert: C-terminal tag, 9 aa linker, strep tag, stop codon
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162310 Copy
Species: Synthetic
Genetic Insert: C-terminal tag, stop codon
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162312 Copy
Species: Synthetic
Genetic Insert: N-terminal tag, HiBiT-SUMO-[TEV cleavage site]
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162301 Copy
Species: Synthetic
Genetic Insert: N-terminal tag, [S-tag]-[TEV cleavage site]
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162302 Copy
Species: Synthetic
Genetic Insert: C-terminal tag, HiBiT-stop, frame1 (NNT-TCG)
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162307 Copy
Species: Synthetic
Genetic Insert: CDS, TEV protease (S219V), no stop codon
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162306 Copy
Species: Halothiobacillus neapolitanus
Genetic Insert: DabAB2 inorganic carbon transport complex
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pFA (derived from pZA31 under PLtet0-1 and constitutive tetR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33084575
Comments: Reported in Desmarais et al. Nat. Micro. 2019.
Proper citation: RRID:Addgene_162690 Copy
Species: Other
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pFA31 (derived from pZA31 expressing under PLtet0-1 and constitutive tetR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33084575
Proper citation: RRID:Addgene_162689 Copy
Species: Halothiobacillus neapolitanus
Genetic Insert: Second CCM operon cloned from H. neapolitanus
Vector Backbone Description: Backbone Size:2980; Vector Backbone:pFA31 (derived from pZA31 expressing under PLtet0-1 and constitutive tetR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33084575
Comments: Inactivation of CbbQ subunit of CbbOQ rubisco activase in pCCM'
Proper citation: RRID:Addgene_162711 Copy
Species: Synthetic
Genetic Insert: mCherry_H-NSdbd
Vector Backbone Description: Backbone Size:5351; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_163102 Copy
Species: Synthetic
Genetic Insert: mEGFP_H-NSdbd
Vector Backbone Description: Backbone Size:5351; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_163101 Copy
Species: Synthetic
Genetic Insert: eYFP_H-NSdbd
Vector Backbone Description: Backbone Size:5351; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_163103 Copy
Species: Synthetic
Genetic Insert: mScarlet-I_H-NSdbd
Vector Backbone Description: Backbone Size:5351; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_163108 Copy
Species: Synthetic
Genetic Insert: mNeonGreen_H-NSdbd
Vector Backbone Description: Backbone Size:5351; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_163109 Copy
Vector Backbone Description: Backbone Size:7484; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11483365
Comments: pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli.
A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3277. The depositing scientist says this deletion has no impact on the function of the plasmid.
Proper citation: RRID:Addgene_28039 Copy
Vector Backbone Description: Backbone Size:7240; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11483365
Comments: Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.
Proper citation: RRID:Addgene_28033 Copy
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