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Genetic Insert: PhiC31o integrase
Vector Backbone Description: Vector Backbone:pET; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52491 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13854; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52498 Copy
Species: Synthetic
Genetic Insert: eNpHR 3.0-EYFP
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52496 Copy
Species: Chlamydomonas
Genetic Insert: Channelrhodopsin-2
Vector Backbone Description: Backbone Size:4606; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24674867
Comments: For inhibition of neuronal activity, please consider Addgene Plasmid #66709, a light-gated chloride channel with improved anion selectivity (iChloC)
Proper citation: RRID:Addgene_52494 Copy
Genetic Insert: PhiC31o attPx3 (70 bp each)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52539 Copy
Genetic Insert: PhiC31o attPx3 (70 bp each)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:10877; Vector Backbone:pCASPER5; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24748663
Proper citation: RRID:Addgene_52533 Copy
Genetic Insert: PhiC31o attPx3 (70 bp each)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52542 Copy
Genetic Insert: PhiC31o attP (full length)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52543 Copy
Genetic Insert: PhiC31o attPx3 (70 bp each)
Vector Backbone Description: Vector Backbone:pRosa26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52546 Copy
Genetic Insert: PhiC31o attP (full length)
Vector Backbone Description: Vector Backbone:pHipp11; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52545 Copy
Genetic Insert: PhiC31o attB (full length)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52554 Copy
Genetic Insert: Lambda integrase attL1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52551 Copy
Genetic Insert: PhiC31o attB (full length)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52552 Copy
Species: Synthetic
Genetic Insert: eYFP and Blasticigin resistance cassette
Vector Backbone Description: Backbone Marker:Thermor Fisher / Fermentas; Backbone Size:2972; Vector Backbone:pJet1.2; Vector Types:blunt cloning kit; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24748663
Proper citation: RRID:Addgene_52559 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF05
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:7831; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23863808
Proper citation: RRID:Addgene_52558 Copy
Species: Homo sapiens
Genetic Insert: HMGCLL1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22865860
Proper citation: RRID:Addgene_52434 Copy
Species: Drosophila melanogaster
Genetic Insert: VGlut-Gal80
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBPGAL80Uw-6; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Addgene's quality control sequencing has found a number of discrepancies with the provided full plasmid sequence. These differences are not thought to affect plasmid function.
Proper citation: RRID:Addgene_52555 Copy
Species: Bacterial origin
Genetic Insert: Kanamycin cassette
Vector Backbone Description: Backbone Size:7145; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is a second generation plasmid that increases the efficiency of sgRNA guide cloning over the original "pLKO.1-puro U6 sgRNA BspMI stuffer" vector.
"pLKO.1-puro U6 sgRNA BfuAI large stuffer" was created to simplify the cloning of the sgRNA guide cassette into pLKO.1-puro U6 sgRNA BspMI stuffer vector originally described in the manuscript “Kearns, NA, etal. Development 2014, 141(1):219-23”. The original vector has a small intervening sequence that presents two problems: 1) the efficiency of excision by BfuAI is moderate, and 2) the size of this spacer is similar to the guide sequence, which makes distinguishing positive clones from reclosures challenging. Consequently, we have inserted a Kanamycin cassette between these sites at a unique Bcl1 site. This element is more efficiently excised by BfuAI and makes distinguishing vectors containing the desired guide sequence easy to distinguish from the parent vector. The excision of the Kan cassette by BfuAI cuts this cassette into 3 fragments due to two internal BfuAI sites within the Kan cassette. Otherwise the cloning of the guides is carried out as described in the manuscript.
Proper citation: RRID:Addgene_52628 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Marker:Bogan lab, unpublished; Backbone Size:5783; Vector Backbone:pB; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11416153
Comments: Plasmid is also called pB-G7. We use FACS to select infected cells that express GFP. There is a short linker between GLUT4 and GFP.
Proper citation: RRID:Addgene_52872 Copy
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