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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5492; Vector Backbone:pRS30x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: LEU2 has been modified to remove ClaI, EcoRI and KpnI sites without altering the amino acid sequence of the Leu2 protein.
Proper citation: RRID:Addgene_35439 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified an insert at bp# 25/26 and a single nucleotide mismatch at bp# 38 when compared to the full plasmid sequence provided by the depositing laboratory. The insert at 25/26 and mismatch at 38 was due to an error made in assembling the promoter and plasmid sequences. Addgene's sequencing results are correct at these locations.
Proper citation: RRID:Addgene_35542 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a deletion at bp# 25 & 26 and a single nucleotide mismatch at bp# 47 when compared to the full plasmid sequence provided by the depositing laboratory. The deletion at 25&26 was due to an error made in assembly of sequence when combining promoter with plasmid sequence. The mismatch at bp#47 is not known to affect plasmid function.
Proper citation: RRID:Addgene_35541 Copy
Species: Mus musculus
Genetic Insert: FoxG1
Vector Backbone Description: Backbone Size:8393; Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22308465
Proper citation: RRID:Addgene_35547 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Proper citation: RRID:Addgene_35546 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 210 when compared to the full plasmid sequence provided by the depositing laboratory. This mismatch is not known to affect the function of the plasmid.
Proper citation: RRID:Addgene_35540 Copy
Species: Synthetic
Genetic Insert: ChR1-VChR1 Chimera
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments:
Proper citation: RRID:Addgene_35499 Copy
Species: Synthetic
Genetic Insert: Rx3(A7)-mEGFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6300; Vector Backbone:pQCXIX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22306808
Proper citation: RRID:Addgene_35531 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB1
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: Mutations in the miR-200b seed regions of the ZEB1 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB1 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Proper citation: RRID:Addgene_35537 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB2 gene
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells.
Science 309:1573-1576
Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
Proper citation: RRID:Addgene_35536 Copy
Species: Homo sapiens
Genetic Insert: miR200c-141 genomic cluster
Vector Backbone Description: Backbone Marker:Scott Hammond; Backbone Size:8749; Vector Backbone:pLenti 4.1Ex; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Proper citation: RRID:Addgene_35534 Copy
Species: Synthetic
Genetic Insert: 7xTOP-GFP
Vector Backbone Description: Backbone Marker:Mark Mercola; Vector Backbone:PGK-H2BmCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318865
Comments: Please note that the gaps between the Addgene sequencing results and the depositor's full sequence do not alter the plasmid function.
Proper citation: RRID:Addgene_35491 Copy
Species: Homo sapiens
Genetic Insert: KRAS(G12V)
Vector Backbone Description: Backbone Marker:Malcom Moore; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318865
Proper citation: RRID:Addgene_35493 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a few nucleotide mismatches at bp# 253, 457, 465, 632, 681 & 1463 when compared to the sequence provided by the depositing laboratory. These differences are not known to affect the function of the plasmid.
Proper citation: RRID:Addgene_35539 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB2 gene
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: Mutations in the miR-200b seed regions of the ZEB2 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells.
Science 309:1573-1576
Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
Proper citation: RRID:Addgene_35538 Copy
Species: Homo sapiens
Genetic Insert: DNMT3L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3L was cloned into EcoRI and XhoI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen
Proper citation: RRID:Addgene_35523 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4514; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: kanMX4 in pRS400 was replaced with natMX4 from pAG25.
Proper citation: RRID:Addgene_35480 Copy
Species: Synthetic
Genetic Insert: Vx3K0
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6300; Vector Backbone:pQCXIX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22306808
Comments: Please note that the C89S mutation makes the mammalian version different than the bacterially expressed version, but this does not change plasmid function.
Proper citation: RRID:Addgene_35527 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4328; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: kanMX4 in pRS400 was replaced with bleMX4 from pUG66.
Proper citation: RRID:Addgene_35478 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments:
Proper citation: RRID:Addgene_35510 Copy
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