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Species: Homo sapiens
Genetic Insert: PSMB1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38600324
Comments: Please note there is a deletion in Gentamycin that causes slightly reduced growth efficiency on (+Amp/Kan/Gen/Tet) compared to cells with a fully WT Gen resistance gene.
Proper citation: RRID:Addgene_214139 Copy
Species: Homo sapiens
Genetic Insert: PSMB1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38600324
Comments: Please note there is a deletion in Gentamycin that causes slightly reduced growth efficiency on (+Amp/Kan/Gen/Tet) compared to cells with a fully WT Gen resistance gene.
Proper citation: RRID:Addgene_214140 Copy
Species: Coxsackievirus B3
Genetic Insert: CVB3
Vector Backbone Description: Vector Backbone:pEN_TTmcs; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39536108
Comments: To maintain the CVB3 internal EcoRI site, this plasmid was created by in a two step procedure: i) PCR cloning of CVB3 from its internal EcoRI site (nucleotides 2764-2769) to the end (nucleotide 7399), digesting with EcoRI-MfeI, and ligating into pEN_TTmcs (Addgene #25755) digested with EcoRI; ii) PCR cloning of CVB3 from its start (nucleotide 1) to the internal EcoRI site, digesting with EcoRI, and ligating into the intermediate plasmid (i) digested with EcoRI.
Proper citation: RRID:Addgene_216788 Copy
Species: Homo sapiens
Genetic Insert: PSMA5
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38600324
Proper citation: RRID:Addgene_217894 Copy
Species: Homo sapiens
Genetic Insert: PSMB1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38600324
Proper citation: RRID:Addgene_217897 Copy
Species: Homo sapiens
Genetic Insert: PSMB5
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38600324
Proper citation: RRID:Addgene_217898 Copy
Species: Homo sapiens
Genetic Insert: codon optimized human KIF1Bß (1770a.a. isoform)
Vector Backbone Description: Vector Backbone:pAcebac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Proper citation: RRID:Addgene_219962 Copy
Species: Synthetic
Genetic Insert: cdGreen2.1
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:4300; Vector Backbone:pAK206; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38724508
Proper citation: RRID:Addgene_221185 Copy
Genetic Insert: Pseudomonas aeruginosa PA14 CRISPR2 locus
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34174210
Proper citation: RRID:Addgene_162318 Copy
Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: Pseudomonas aeruginosa PA14 CRISPR2 locus
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34174210
Proper citation: RRID:Addgene_162319 Copy
Genetic Insert: msfGFP
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:3028; Vector Backbone:pSEVA631; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37975669
Comments: Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint.
Proper citation: RRID:Addgene_208015 Copy
Vector Backbone Description: Backbone Marker:Addgene #60681; Backbone Size:4191; Vector Backbone:pUCX; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36993673
Proper citation: RRID:Addgene_204409 Copy
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209385 Copy
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209386 Copy
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209387 Copy
Species: Rattus norvegicus
Genetic Insert: Rps6kb1
Vector Backbone Description: Backbone Marker:Addgene #2752 ; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24658685
Proper citation: RRID:Addgene_58507 Copy
Species: Mus musculus
Genetic Insert: JunD
Vector Backbone Description: Backbone Marker:Addgene #2752 ; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24658685
Proper citation: RRID:Addgene_58498 Copy
Species: Mus musculus
Genetic Insert: Tgfbr3_HA
Vector Backbone Description: Backbone Marker:Addgene #25752 ; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24658685
Proper citation: RRID:Addgene_58497 Copy
Species: Synechoccus elongatus PCC 7942
Genetic Insert: PkaiB-KaiC-ECFP
Vector Backbone Description: Vector Backbone:PkaiB-kaiC-ecfp expressed from NS2 vector with Gm resistance constructed by seamless assembly.; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:25127213
Proper citation: RRID:Addgene_85121 Copy
Species: Synthetic
Genetic Insert: C321.∆A.opt strain
Vector Backbone Description: Vector Backbone:Whole genome; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28545477
Comments: C321.∆A.opt is a variant of C321.∆A (Addgene Item #48998) engineered to have a faster growth rate, as reported in Kuznetsov, Gleb, et al. "Optimizing complex phenotypes through model-guided multiplex genome engineering." bioRxiv (2016): 086595.
This strain has a heat-inducible lambda prophage (42 C) and should be grown at or below 34 C to avoid induction.
The original strain C321.∆A (Addgene Item #48998) was reported in Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013): 357-360.
Proper citation: RRID:Addgene_87359 Copy
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