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Species: Homo sapiens
Genetic Insert: TMPRSS2
Vector Backbone Description: Vector Backbone:pWPI-IRES-Bla-Ak; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_154982 Copy
Species: Homo sapiens
Genetic Insert: Glutaredoxin-1
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155045 Copy
Species: Lachnospiraceae bacterium
Genetic Insert: LbCas12a
Vector Backbone Description: Backbone Size:7377; Vector Backbone:lenti CRISPR pXPR_001/Lenti_Cas9_2A_Blast (Addgene plasmid #73310); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32249828
Comments: The NeoR/KanR cassette contains S177R and E182D mismatches compared to the reference sequence. The depositing lab does not believe these are of functional concern but the construct should be functionally validated prior to experimentation.
Proper citation: RRID:Addgene_155046 Copy
Species: Homo sapiens
Genetic Insert: CYLD
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDEST-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495026
Proper citation: RRID:Addgene_15506 Copy
Vector Backbone Description: Backbone Size:14744; Vector Backbone:LCV2_mCherry; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32694731
Proper citation: RRID:Addgene_155096 Copy
Vector Backbone Description: Backbone Size:14747; Vector Backbone:LCV2_EGFP; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32694731
Proper citation: RRID:Addgene_155098 Copy
Species: Homo sapiens
Genetic Insert: pCDNA3 Flag MKK4beta
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7839144
Proper citation: RRID:Addgene_15517 Copy
Species: Rattus norvegicus
Genetic Insert: p70 S6K1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:HA-GST-pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17386266
Proper citation: RRID:Addgene_15511 Copy
Species: Bifidobacterium adolescentis
Genetic Insert: Sucrose Phosphorylase from Bifidobacterium adolescentis
Vector Backbone Description: Backbone Size:3060; Vector Backbone:self assembled; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32668097
Proper citation: RRID:Addgene_155165 Copy
Genetic Insert: Barcode library
Vector Backbone Description: Backbone Marker:Aviv Regev and Joan Brugge; Vector Backbone:Plasmid 155258: Watermelon backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34381210
Proper citation: RRID:Addgene_155257 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32518240
Comments: Strain is described in PMID: 32518240. Genotype: ilvG rfb-50 rph-1 ΔrrnGADEHBC DEL(recA)/ ptRNA100, pA15, SpcR, alaT, aspT, ileT, trpT, gltT / pCSacB. pCSacB description (PMID: 26438293 and PMID:14617656). Strain is also resistant to spectinomycin,
Proper citation: RRID:Addgene_155206 Copy
Species: Homo sapiens
Genetic Insert: hACE2
Vector Backbone Description: Vector Backbone:pLenti CMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155295 Copy
Species: Homo sapiens
Genetic Insert: hACE2
Vector Backbone Description: Vector Backbone:pLenti CMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34376481
Proper citation: RRID:Addgene_155296 Copy
Species: SARS-Cov2
Genetic Insert: SARS-Cov2 spike_deleted
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Generation of SARS-CoV-2-pseudotyped lentiviral particles; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34547465
Proper citation: RRID:Addgene_155297 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155221 Copy
Vector Backbone Description: Backbone Size:9512; Vector Backbone:Addgene #155182; Vector Types:Mammalian Expression, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Comments: The T2A-Puro sequence was re-amplified and cloned into the backbone to remove BbsI restriction site in the T2A sequence. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns and Ms are the gRNA sequence and its reverse-complement counterpart:
FWD:5'- AAACNNNNNNNNNNNNN
RVS: 5'-AAAAMMMMMMMMMMM
Proper citation: RRID:Addgene_155184 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155220 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15553 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15551 Copy
Species: Homo sapiens
Genetic Insert: FKBP12(V36)-p30Caspase9
Vector Backbone Description: Backbone Size:5300; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728125
Comments: Based on L. Fan et al (99) Human Gene Therapy
Proper citation: RRID:Addgene_15567 Copy
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