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Species: Homo sapiens
Genetic Insert: MAP1LC3B
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Comments: 3' codons have been altered from original reference sequence, while preserving the amino acid sequence.
Proper citation: RRID:Addgene_137757 Copy
Species: Homo sapiens
Genetic Insert: GABARAP
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137760 Copy
Species: Homo sapiens
Genetic Insert: CD44s (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Comments: Addgene NGS result found mutations I186T and I253V when compared to CD44 [XP_016874074.1]. Depositor confirmed that this does not affect the function of the plasmid.
Proper citation: RRID:Addgene_137812 Copy
Species: Homo sapiens
Genetic Insert: CD44v3 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137814 Copy
Species: Homo sapiens
Genetic Insert: Human GRK6[L66A E514A]
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137781 Copy
Species: Homo sapiens
Genetic Insert: mir30 cassette
Vector Backbone Description: Backbone Size:5555; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre.
Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)).
Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.
Proper citation: RRID:Addgene_13786 Copy
Species: Homo sapiens
Genetic Insert: CD44v6-10 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137820 Copy
Species: Homo sapiens
Genetic Insert: CD44v2-10 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Comments: Addgene NGS result found mutations A282T, T483A, N535D, S549C in V2-V10 region. Depositor confirmed that this does not affect the function of the plasmid.
Proper citation: RRID:Addgene_137824 Copy
Species: Homo sapiens
Genetic Insert: CD44v8-10 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137823 Copy
Species: Homo sapiens
Genetic Insert: AO7 aa 126-258
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26475854
Proper citation: RRID:Addgene_137843 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Vector Backbone:pX330S-2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137866 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Vector Backbone:pGEP179_pX330K; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137865 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Vector Backbone:pX330S-3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137867 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Vector Backbone:pX330S-2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137869 Copy
Species: Homo sapiens
Genetic Insert: POGZ
Vector Backbone Description: Vector Backbone:pGEP179_pX330K; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137872 Copy
Species: Homo sapiens
Genetic Insert: EYA1
Vector Backbone Description: Vector Backbone:pWCC43; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138003 Copy
Species: Homo sapiens
Genetic Insert: 3xMYC tagged SIX1
Vector Backbone Description: Vector Backbone:pWCC43; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138004 Copy
Species: Homo sapiens
Genetic Insert: BirA* tagged SIX2
Vector Backbone Description: Vector Backbone:pWCC43; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138007 Copy
Species: Homo sapiens
Genetic Insert: Jnk2a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7969172
Proper citation: RRID:Addgene_13801 Copy
Species: Homo sapiens
Genetic Insert: SIX1 sgRNA13
Vector Backbone Description: Vector Backbone:pTJK459; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138014 Copy
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