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http://www.addgene.org/23926

Species: Homo sapiens
Genetic Insert: EPHA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Proper citation: RRID:Addgene_23926 Copy   

•   Source: Addgene

http://www.addgene.org/23922

Species: Homo sapiens
Genetic Insert: FGFR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: Please note that Addgene's sequencing results identifies mismatches at T631G and C1052T compared to the FGFR1 sequence provided, resulting in W211G and S351F mutations. The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Proper citation: RRID:Addgene_23922 Copy   

•   Source: Addgene

http://www.addgene.org/23925

Species: Homo sapiens
Genetic Insert: KDR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Proper citation: RRID:Addgene_23925 Copy   

•   Source: Addgene

http://www.addgene.org/23888

Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Proper citation: RRID:Addgene_23888 Copy   

•   Source: Addgene

http://www.addgene.org/24028

Vector Backbone Description: Backbone Size:5028; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag4H-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag4H.

Proper citation: RRID:Addgene_24028 Copy   

•   Source: Addgene

http://www.addgene.org/24020

Vector Backbone Description: Backbone Size:6191; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Comments: Unpublished (variant of constructs described in Scahill et al 2008). For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html

Proper citation: RRID:Addgene_24020 Copy   

•   Source: Addgene

http://www.addgene.org/24021

Vector Backbone Description: Backbone Size:5753; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Comments: Unpublished (variant of constructs described in Scahill et al 2008). For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html

Proper citation: RRID:Addgene_24021 Copy   

•   Source: Addgene

http://www.addgene.org/23988

Species: Homo sapiens
Genetic Insert: NFATc1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCR4; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16740479
Comments: The c1nuc plasmid expresses the constitutively nuclear form of NFATc1 (Ho et al Nature 2004). It contains mutations in the phosphorylation sites for Dyrk1a and GSK3 that are responsible for actively exporting the protein from the nucleus (Beal et al Science 1997; Arron et al Nature 2006). It can be used as a transgene to explore NFAT signaling (Winslow et al Dev Cell 2006) and when expressed in a specific tissue can direct events such as bone development, thymocyte development or pancreatic beta cell functions (Heit J et al Nature 2007).

Proper citation: RRID:Addgene_23988 Copy   

•   Source: Addgene

http://www.addgene.org/24037

Species: Saccharomyces cerevisiae
Genetic Insert: RPL25
Vector Backbone Description: Backbone Size:5000; Vector Backbone:yEGFP3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Entire RPL25 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3

Proper citation: RRID:Addgene_24037 Copy   

•   Source: Addgene

http://www.addgene.org/24032

Vector Backbone Description: Backbone Size:5099; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag43M-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag43M. Based on pMOTag series made in pBluescript II KS+

Proper citation: RRID:Addgene_24032 Copy   

•   Source: Addgene

http://www.addgene.org/24007

Genetic Insert: T7 RNA Polymerase
Vector Backbone Description: Backbone Size:6000; Vector Backbone:N/A; Vector Types:Trypanosome Expression; Bacterial Resistance:Ampicillin
Comments: T7 RNA Polymerase and Tet Repressor present (see map). Target locus: Tubulin Regulation: None

Proper citation: RRID:Addgene_24007 Copy   

•   Source: Addgene

http://www.addgene.org/24014

Vector Backbone Description: Backbone Marker:George Cross; Backbone Size:6145; Vector Backbone:pHD309-HYG; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Targeting: B-Tubulin Regulation: None For more information see George Cross's website: http://tryps.rockefeller.edu/trypsru2_plasmids.html

Proper citation: RRID:Addgene_24014 Copy   

•   Source: Addgene

http://www.addgene.org/24069

Species: Mus musculus
Genetic Insert: Rorgt
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_24069 Copy   

•   Source: Addgene

http://www.addgene.org/24066

Species: Mus musculus
Genetic Insert: IL23R
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: IL23R cDNA amplified through IL6 treated cDNA as 2 parts (5' part ATG-134-BamHI; 3' part 134-STOP). 5' part cloned into pcDNA3 XhoI/BamHI 3' part cloned into MSCV hCD2 Part linked together in pcDNA3 (XhoI-MluI) Then cut with XhoI/NruI cloned into pMIGR (XhoI/HpaI)

Proper citation: RRID:Addgene_24066 Copy   

•   Source: Addgene

http://www.addgene.org/24071

Species: Mus musculus
Genetic Insert: Foxp3
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:7894; Vector Backbone:MSCV-LTRmiR30-PIG (LMP); Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18368049
Comments: The target sequence of Foxp3: 5′-GGCAGAGGACACTCAATGAAAT-3′

Proper citation: RRID:Addgene_24071 Copy   

•   Source: Addgene

http://www.addgene.org/24074

Species: Homo sapiens
Genetic Insert: HP1 alpha
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11959914

Proper citation: RRID:Addgene_24074 Copy   

•   Source: Addgene

http://www.addgene.org/24264

Species: Homo sapiens
Genetic Insert: Bmf
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12591950

Proper citation: RRID:Addgene_24264 Copy   

•   Source: Addgene

http://www.addgene.org/24153

Species: Homo sapiens
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Matthias et al, Nucleic Acid Research (1989).; Backbone Size:5120; Vector Backbone:modified pEV3S (pLG-033); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16135789
Comments: FOr more details for this expression construct as well as other mutants, please contact Dr Lin-Feng CHen ([email protected])

Proper citation: RRID:Addgene_24153 Copy   

•   Source: Addgene

http://www.addgene.org/24129

Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:7000; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427958
Comments: pUltra was cloned by Yildirim Dogan ([email protected]). It is described in the associated article (PMID 22427958) and also in PMID 24630793. This is a 3rd generation Lentiviral vector with an internal Ubiquitinc Promoter. By cloning into the compatible cloning sites* (XbaI and BamHI) downstream of EGFP-P2A, you get a bi-cistronic expression of EGFP and the gene of interest. You can clone a second gene of interest into the NheI/bclI**donwstream of EGFP-P2A-gene1-T2A and get a multi-cistronic expression of all three genes. * compatible cloning site (ccs): you can pcr amplifiy your gene of interest with a forward primer including one of these cutting sites: SpeI or NheI or XbaI and a reverse primer with one these cutting sites: BglII or BamHI or bclI. The PCR product is now compatible with the first site as well the 2nd site. "You can mix and match". ** for bclI the vector has to be amplified by dam-methylation defective E coli strains (e.g. SC110 Stratagene). Further advantage of the vector is, that you can simultaneously do RNAi by cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR. Plus while integration the RNAi cassette gets doubled, since the 5´LTR is dublicated while reverse transcripton from the 3´-LTR ***P2A: 3984-4046 bp and T2A: 4062-4124 bp

Proper citation: RRID:Addgene_24129 Copy   

•   Source: Addgene

http://www.addgene.org/24225

Species: Homo sapiens
Genetic Insert: E2F1
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pCMVHANeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8622649
Comments: Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest. This plasmid contains an E356K mutation within the E2F1 insert. Please note that other mutations may exist and it is unclear whether this mutation affects plasmid function.

Proper citation: RRID:Addgene_24225 Copy   

•   Source: Addgene