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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDONR223-EPHA2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_23926 | EPHA2 | Homo sapiens | Spectinomycin | PMID:21107320 | The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | 2026-08-15 01:12:27 | 5 | |
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pDONR223-FGFR1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_23922 | FGFR1 | Homo sapiens | Spectinomycin | PMID:21107320 | Please note that Addgene's sequencing results identifies mismatches at T631G and C1052T compared to the FGFR1 sequence provided, resulting in W211G and S351F mutations. The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | W211G and S351F compared to insert sequence provided | 2026-08-15 01:12:26 | 2 |
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pDONR223-KDR Resource Report Resource Website 1+ mentions |
RRID:Addgene_23925 | KDR | Homo sapiens | Spectinomycin | PMID:21107320 | The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | V297I | 2026-08-15 01:12:26 | 2 |
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pDONR223-ERBB2 Resource Report Resource Website 10+ mentions |
RRID:Addgene_23888 | ERBB2 | Homo sapiens | Spectinomycin | PMID:21107320 | The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | 2026-08-15 01:12:25 | 11 | |
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pMOTag4H-crelox-PUR Resource Report Resource Website 1+ mentions |
RRID:Addgene_24028 | Ampicillin | pMOTag4H-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag4H. | Backbone Size:5028; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:28 | 1 | ||||
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pyrFEKO-HYG Resource Report Resource Website 1+ mentions |
RRID:Addgene_24020 | Ampicillin | Unpublished (variant of constructs described in Scahill et al 2008). For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html | Backbone Size:6191; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 2 | ||||
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pyrFEKO-PUR Resource Report Resource Website 1+ mentions |
RRID:Addgene_24021 | Ampicillin | Unpublished (variant of constructs described in Scahill et al 2008). For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html | Backbone Size:5753; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 1 | ||||
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pCR4-huNFATc1nuc Resource Report Resource Website 1+ mentions |
RRID:Addgene_23988 | NFATc1 | Homo sapiens | Ampicillin | PMID:16740479 | The c1nuc plasmid expresses the constitutively nuclear form of NFATc1 (Ho et al Nature 2004). It contains mutations in the phosphorylation sites for Dyrk1a and GSK3 that are responsible for actively exporting the protein from the nucleus (Beal et al Science 1997; Arron et al Nature 2006). It can be used as a transgene to explore NFAT signaling (Winslow et al Dev Cell 2006) and when expressed in a specific tissue can direct events such as bone development, thymocyte development or pancreatic beta cell functions (Heit J et al Nature 2007). | Backbone Size:4000; Vector Backbone:pCR4; Vector Types:cloning vector; Bacterial Resistance:Ampicillin | S172A S175A S176A S178A S179A S181A S184A S187A S188A S191A S194A S199A S203A S207A S211A S233A S237A S278A S282A S286A S290A | 2026-08-15 01:12:26 | 3 |
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RPL25-GFP (94) Resource Report Resource Website 1+ mentions |
RRID:Addgene_24037 | RPL25 | Saccharomyces cerevisiae | Ampicillin | Entire RPL25 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3 | Backbone Size:5000; Vector Backbone:yEGFP3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 2 | ||
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pMOTag43M-crelox-PUR Resource Report Resource Website 1+ mentions |
RRID:Addgene_24032 | Ampicillin | pMOTag43M-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag43M. Based on pMOTag series made in pBluescript II KS+ | Backbone Size:5099; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 1 | ||||
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pLEW13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24007 | T7 RNA Polymerase | Ampicillin | T7 RNA Polymerase and Tet Repressor present (see map). Target locus: Tubulin Regulation: None | Backbone Size:6000; Vector Backbone:N/A; Vector Types:Trypanosome Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 1 | |||
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pHD309-HYG-PUR Resource Report Resource Website 1+ mentions |
RRID:Addgene_24014 | Ampicillin | Targeting: B-Tubulin Regulation: None For more information see George Cross's website: http://tryps.rockefeller.edu/trypsru2_plasmids.html | Backbone Marker:George Cross; Backbone Size:6145; Vector Backbone:pHD309-HYG; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 2 | ||||
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MIGR-RORgt Resource Report Resource Website 1+ mentions |
RRID:Addgene_24069 | Rorgt | Mus musculus | Ampicillin | Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:28 | 9 | |||
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MIGR-IL23R Resource Report Resource Website 1+ mentions |
RRID:Addgene_24066 | IL23R | Mus musculus | Ampicillin | IL23R cDNA amplified through IL6 treated cDNA as 2 parts (5' part ATG-134-BamHI; 3' part 134-STOP). 5' part cloned into pcDNA3 XhoI/BamHI 3' part cloned into MSCV hCD2 Part linked together in pcDNA3 (XhoI-MluI) Then cut with XhoI/NruI cloned into pMIGR (XhoI/HpaI) | Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 1 | ||
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LMP 1066 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24071 | Foxp3 | Mus musculus | Ampicillin | PMID:18368049 | The target sequence of Foxp3: 5′-GGCAGAGGACACTCAATGAAAT-3′ | Backbone Marker:Open Biosystems; Backbone Size:7894; Vector Backbone:MSCV-LTRmiR30-PIG (LMP); Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 3 | |
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GST HP1 alpha Resource Report Resource Website 1+ mentions |
RRID:Addgene_24074 | HP1 alpha | Homo sapiens | Ampicillin | PMID:11959914 | Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:27 | 2 | ||
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pGSTag Bmf Resource Report Resource Website 1+ mentions |
RRID:Addgene_24264 | Bmf | Homo sapiens | Ampicillin | PMID:12591950 | Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:30 | 1 | ||
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T7-RelA(S276A) Resource Report Resource Website 1+ mentions |
RRID:Addgene_24153 | RelA | Homo sapiens | Ampicillin | PMID:16135789 | FOr more details for this expression construct as well as other mutants, please contact Dr Lin-Feng CHen ([email protected]) | Backbone Marker:Matthias et al, Nucleic Acid Research (1989).; Backbone Size:5120; Vector Backbone:modified pEV3S (pLG-033); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S276A | 2026-08-15 01:12:28 | 2 |
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pUltra Resource Report Resource Website 50+ mentions |
RRID:Addgene_24129 | Ampicillin | PMID:22427958 | pUltra was cloned by Yildirim Dogan ([email protected]). It is described in the associated article (PMID 22427958) and also in PMID 24630793. This is a 3rd generation Lentiviral vector with an internal Ubiquitinc Promoter. By cloning into the compatible cloning sites* (XbaI and BamHI) downstream of EGFP-P2A, you get a bi-cistronic expression of EGFP and the gene of interest. You can clone a second gene of interest into the NheI/bclI**donwstream of EGFP-P2A-gene1-T2A and get a multi-cistronic expression of all three genes. * compatible cloning site (ccs): you can pcr amplifiy your gene of interest with a forward primer including one of these cutting sites: SpeI or NheI or XbaI and a reverse primer with one these cutting sites: BglII or BamHI or bclI. The PCR product is now compatible with the first site as well the 2nd site. "You can mix and match". ** for bclI the vector has to be amplified by dam-methylation defective E coli strains (e.g. SC110 Stratagene). Further advantage of the vector is, that you can simultaneously do RNAi by cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR. Plus while integration the RNAi cassette gets doubled, since the 5´LTR is dublicated while reverse transcripton from the 3´-LTR ***P2A: 3984-4046 bp and T2A: 4062-4124 bp | Backbone Marker:Didier Trono; Backbone Size:7000; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:29 | 89 | |||
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pCMVHA E2F1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_24225 | E2F1 | Homo sapiens | Ampicillin | PMID:8622649 | Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest. This plasmid contains an E356K mutation within the E2F1 insert. Please note that other mutations may exist and it is unclear whether this mutation affects plasmid function. | Backbone Size:6200; Vector Backbone:pCMVHANeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:28 | 32 |
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