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Species: Homo sapiens
Genetic Insert: RBPMS
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5365; Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24860013
Proper citation: RRID:Addgene_65741 Copy
Species: Homo sapiens
Genetic Insert: ELAVL4
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:2279; Vector Backbone:pENTR 4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65754 Copy
Species: Homo sapiens
Genetic Insert: ELAVL3
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:2279; Vector Backbone:pENTR 4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65753 Copy
Species: Homo sapiens
Genetic Insert: SRM1
Vector Backbone Description: Backbone Marker:Life Technologies, modified by the Tuschl lab; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Primers used to amplify 3' UTR from human cDNA (HCC1964 cDNA):
SRM1 forward: AAA AAG CAG GCT TCG CCC AGG CGC CAC CAC T
SRM1 reverse: AGA AAG CTG GGT GGC TAT AAA TAC ACG TGT T
Proper citation: RRID:Addgene_65866 Copy
Genetic Insert: CA repeats 50-100 nucleotides in lngth
Vector Backbone Description: Backbone Marker:Life Technologies, modified by the Tuschl lab; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65867 Copy
Species: Homo sapiens
Genetic Insert: ANKRD46
Vector Backbone Description: Backbone Marker:Life Technologies, modified by the Tuschl lab; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65861 Copy
Species: Homo sapiens
Genetic Insert: UBE2V1
Vector Backbone Description: Backbone Marker:Life Technologies, modified by the Tuschl lab; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Primers used to amplify 3' UTR from human cDNA (HCC1964 cDNA):
UBE2V1 forward: AAA AAG CAG GCT TCT CAA AAA GAA AAA CCA CAG
UBE2V1 reverse: AGA AAG CTG GGT GTT AAA TAT TAA CGC ACA CT
Proper citation: RRID:Addgene_65864 Copy
Species: Homo sapiens
Genetic Insert: NDUFA6
Vector Backbone Description: Backbone Marker:Life Technologies, modified by the Tuschl lab; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65863 Copy
Species: Synthetic
Genetic Insert: FACS Controls
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Proper citation: RRID:Addgene_66065 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66101 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology, Other, Destination Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66069 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chronos
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66102 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology, Other, Destination Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66067 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66071 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Proper citation: RRID:Addgene_66072 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029110. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66188 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029113 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66190 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029112 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66191 Copy
Species: Homo sapiens
Genetic Insert: XRN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4174; Vector Backbone:pLNHA-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23142987
Proper citation: RRID:Addgene_66596 Copy
Species: Synthetic
Genetic Insert: copGFP
Vector Backbone Description: Backbone Size:10214; Vector Backbone:pC13N-iRMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25587899
Proper citation: RRID:Addgene_66578 Copy
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