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Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K. l. URA3 477..1277
P K. l. URA3 1278..1776
loxP 1793..1826
Consensus Ty1Cons2 3' 1966..2171
pUC ori 2945..3588
AmpR 3691..4549
Consensus Ty1Cons2 5' 4993..5224
T ADH1 5251..5445
P TEF1 5464..5883
GFP 5893..6609
Proper citation: RRID:Addgene_63651 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. URA3 477..1277
P K.l. URA3 1278..1776
loxP 1793..1826
Consensus Ty3 3' 1966..2166
pUC ori 2947..3590
AmpR 3693..4551
Consensus Ty3 5' 4995..5228
T ADH1 5255..5449
P TEF1 5468..5887
GFP 5897..6613
Proper citation: RRID:Addgene_63653 Copy
Species: Other
Genetic Insert: PhlF and LacI
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63813 Copy
Species: Other
Genetic Insert: PhlF and LacI
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63814 Copy
Species: Other
Genetic Insert: rrsA, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:BAC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_63837 Copy
Species: Other
Genetic Insert: rrsB, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:BAC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_63838 Copy
Species: Other
Genetic Insert: gpmM, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63841 Copy
Species: Other
Genetic Insert: ftsZ, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63842 Copy
Species: Other
Genetic Insert: IKBKB
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: CGAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-C2-STOP
Proper citation: RRID:Addgene_64609 Copy
Species: Other
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X2-V5
Proper citation: RRID:Addgene_64649 Copy
Species: Other
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X2-STOP
Proper citation: RRID:Addgene_64648 Copy
Species: Other
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TACT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X1-V5
Proper citation: RRID:Addgene_64647 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65143 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6576.
Additional article references: Le Guiner et al. Mol. Ther. 22:1923-1935 (2014)
Proper citation: RRID:Addgene_65216 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6871.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6569.
Proper citation: RRID:Addgene_65213 Copy
Species: Other
Genetic Insert: VSV-G
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: cytoplasmic tagging
Proper citation: RRID:Addgene_65301 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART HcKan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65316 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART HcKan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65315 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART HcKan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65314 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65326 Copy
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