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Species: Homo sapiens
Genetic Insert: SUZ12
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCMVHANeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15385962
Proper citation: RRID:Addgene_24232 Copy
Species: Aequorea victora (modified) / synthetic
Genetic Insert: 7xTcf-eGFP // SV40-mCherry
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6132; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: TCF repeat sequence is AGATCAAAGG
This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24304 Copy
Species: Aequorea victoria (modified) / Streptomyces alboniger
Genetic Insert: 7xTcf-eGFP // SV40-PuroR
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6132; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: TCF repeat sequence is AGATCAAAGGgg.
This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24305 Copy
Genetic Insert: mCD8-GFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pUAS-mCD8-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The FRT-stop-FRT cassette, with additional BglII/NotI restriction sites, was PCR amplified from genomic DNA of UAS-FRT-stop-FRT-shibire (ts) flies and cloned into pUAST-mCD8-gfp to generate pUAST-FRT-stop-FRT-mCD8-gfp (pUAS>stop>mCD8-GFP)
Proper citation: RRID:Addgene_24385 Copy
Species: Mus musculus
Genetic Insert: EF1alpha-betacatenin(deltaGSK) // SV40-PuroR
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6074; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24313 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24402 Copy
Species: Neurospora crassa
Genetic Insert: QS
Vector Backbone Description: Backbone Marker:Bischof et al., 2007; Backbone Size:9000; Vector Backbone:pUAST attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated
into a blunted pUASTattB (Bischof et al., 2007) which had been digested with BglII and KpnI.
Proper citation: RRID:Addgene_24366 Copy
Species: Neurospora crassa
Genetic Insert: QF
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pattB-hsp70; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: A newer version of this plasmid is now available - pattB-hsp70P-QF#7-hsp70T www.addgene.org/46134 (Addgene plasmid # 46134)
Proper citation: RRID:Addgene_24368 Copy
Genetic Insert: mCD8-GFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The FRT-Stop-FRT cassette from pUAS>Stop>mCD8-GFP was
excised by BglII/NotI digestion. The mCD8-GFP cassette was excised from pQUAST-mCD8GFP by NotI digestion. The two inserts were ligated into the BglII/NotI sites of the pQUAST vector.
Proper citation: RRID:Addgene_24361 Copy
Species: Homo sapiens
Genetic Insert: GSK3beta(S9A)
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: Addgene's sequence results have identified a duplicated mRFP1 and GSK S9A in this plasmid. The depositor has confirmed that this duplication should not impact plasmid functionality.
Proper citation: RRID:Addgene_24371 Copy
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pPac5c-PL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: GAL80 cDNA was PCR amplified using primers PR511
(CCCCGGATCCCAACatggactacaacaagagatcttcgg) and PR512
(CGGTTAACGCGGCCGCttataaactataatgcgagatattgctaacg), and a lab vector, pCA-GAL80 as the
template. The PCR product was cloned using BamHI and NotI into pPAC5C-PL.
Proper citation: RRID:Addgene_24346 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Proper citation: RRID:Addgene_24327 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.
Proper citation: RRID:Addgene_24333 Copy
Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
Proper citation: RRID:Addgene_24334 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:20350301
Comments: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA]
Proper citation: RRID:Addgene_24545 Copy
Species: Homo sapiens
Genetic Insert: transcription factor Sp3
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.
Proper citation: RRID:Addgene_24541 Copy
Genetic Insert: pN3-Control
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pN3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: vector control for pN3-Sp1FL and pN3-Sp3FL vectors
Proper citation: RRID:Addgene_24544 Copy
Species: Homo sapiens
Genetic Insert: Sp1FL
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pN3-Control vector also available from Addgene
2 nucleotide mismatches with author sequence detected in the most 3´-UTR. They do not affect the coding sequence of Sp1.
Proper citation: RRID:Addgene_24543 Copy
Species: A. victoria
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:4557; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24557 Copy
Genetic Insert: Sleeping Beauty
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3712; Vector Backbone:pCMVbeta; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390559
Proper citation: RRID:Addgene_24551 Copy
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