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Species: Other
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pPC047; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the ccdB gene was used for screening positive transformants(if inserts failed to replace ccdB, cells would not grow in DH5alpha)
Proper citation: RRID:Addgene_65328 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65325 Copy
Species: Other
Genetic Insert: TEF2p-ycrtI-ADH1t
Vector Backbone Description: Vector Backbone:POT4; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: construction of TUs(TEF2p-ycrtI-ADH1t) in POT4 receiving vector
Proper citation: RRID:Addgene_65356 Copy
Species: Other
Genetic Insert: CYC1p-ycrtI-ADH1t
Vector Backbone Description: Vector Backbone:POT4; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: construction of TUs(CYC1p-ycrtI-ADH1t) in POT4 receiving vector
Proper citation: RRID:Addgene_65357 Copy
Species: Other
Genetic Insert: TDH3p-ycrtE-ADH1t
Vector Backbone Description: Vector Backbone:POT2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: construction of TUs(TDH3p-ycrtE-ADH1t) in POT2 receiving vector
Proper citation: RRID:Addgene_65352 Copy
Species: Other
Genetic Insert: CYC1p-ycrtE-ADH1t
Vector Backbone Description: Vector Backbone:POT2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: construction of TUs(CYC1p-ycrtE-ADH1t) in POT2 receiving vector
Proper citation: RRID:Addgene_65354 Copy
Species: Other
Genetic Insert: TEF2p-ycrtE-ADH1t
Vector Backbone Description: Vector Backbone:POT2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: construction of TUs(TEF2p-ycrtE-ADH1t) in POT2 receiving vector
Proper citation: RRID:Addgene_65353 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65767 Copy
Species: Other
Genetic Insert: mScarlet3-H
Vector Backbone Description: Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_242971 Copy
Species: Other
Genetic Insert: Giantin-mScarlet3-H
Vector Backbone Description: Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_242973 Copy
Species: Other
Genetic Insert: type I-E CRISPR cas crRNA
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41290661
Proper citation: RRID:Addgene_247075 Copy
Species: Other
Genetic Insert: type I-E CRISPR cas crRNA
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41290661
Proper citation: RRID:Addgene_247076 Copy
Species: Other
Genetic Insert: type I-E CRISPR cas crRNA
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41290661
Proper citation: RRID:Addgene_247080 Copy
Species: Other
Genetic Insert: type I-E CRISPR cas crRNA
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41290661
Comments: The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds amino acids 56-95 deleted in AraC (WP_407234930.1).
Proper citation: RRID:Addgene_247079 Copy
Species: Other
Genetic Insert: rtTA
Vector Backbone Description: Vector Backbone:Galloway Lab pSHIP Backbone; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41083901
Comments: Also contains a co-transcribed tagBFP . Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.
Proper citation: RRID:Addgene_246372 Copy
Species: Other
Genetic Insert: ABEmax7.10-SpRY (SpRY-ABE)
Vector Backbone Description: Vector Backbone:pIVTRup(BsmBI); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40993398
Comments: Source insert Addgene ID 140003
Proper citation: RRID:Addgene_242539 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pIVTRup; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40993398
Comments: Due to the difficulties of maintaining and sequencing long homopolymers, Addgene cannot guarantee the exact length of polyA sequence.
Proper citation: RRID:Addgene_242545 Copy
Species: Other
Genetic Insert: EBFP2
Vector Backbone Description: Vector Backbone:pIVTRup; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40993398
Proper citation: RRID:Addgene_242544 Copy
Species: Other
Genetic Insert: mCherry2
Vector Backbone Description: Vector Backbone:LentiGuide-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40993398
Proper citation: RRID:Addgene_242529 Copy
Species: Other
Genetic Insert: rtTA
Vector Backbone Description: Vector Backbone:Galloway Lab pSHIP Backbone; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41083901
Comments: Also contains a co-transcribed mRuby2 . Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.
Proper citation: RRID:Addgene_246373 Copy
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