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Species: Other
Genetic Insert: iRFP670
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41083901
Comments: Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.
Proper citation: RRID:Addgene_246374 Copy
Species: Other
Genetic Insert: gRNA targeting NRF1-Ex7 splice site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237466 Copy
Species: Other
Genetic Insert: Intergenic sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237464 Copy
Species: Other
Genetic Insert: Intergenic sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237465 Copy
Species: Other
Genetic Insert: gRNA targeting NRF1-Ex7 splice site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237468 Copy
Species: Other
Genetic Insert: TadA8e-nSpCas9(D10A)
Vector Backbone Description: Backbone Marker:Caixia Gao, Addgene Plasmid #98164; Vector Backbone:pnCas9-PBE; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39928528
Proper citation: RRID:Addgene_238020 Copy
Species: Other
Genetic Insert: B/c
Vector Backbone Description: Backbone Size:2617; Vector Backbone:pENTR; Vector Types:; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:41085227
Proper citation: RRID:Addgene_234368 Copy
Species: Other
Genetic Insert: B/c
Vector Backbone Description: Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:41085227
Proper citation: RRID:Addgene_234369 Copy
Species: Other
Genetic Insert: QUAS enhancer driving mNeonGreen-NES and mKate2-NLS
Vector Backbone Description: Backbone Size:7839; Vector Backbone:pDestTol2CR; Vector Types:zebrafish Tol2 transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41123438
Proper citation: RRID:Addgene_241912 Copy
Species: Other
Genetic Insert: CitA-Tq-LR
Vector Backbone Description: Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41071660
Proper citation: RRID:Addgene_241830 Copy
Species: Other
Genetic Insert: Alb-mStayGold
Vector Backbone Description: Backbone Marker:VVF (p438) https://vvf.ethz.ch/ ; Vector Backbone:pAAV-P3 (Addgene plasmid #183460); Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41393514
Proper citation: RRID:Addgene_234549 Copy
Species: Other
Genetic Insert: φC31(PhiC31)
Vector Backbone Description: Backbone Marker:NA; Backbone Size:5218; Vector Backbone:pET28a(+)v2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease).
The total insert size displayed on this page (2403bp) includes all tags and linkers.
Proper citation: RRID:Addgene_246982 Copy
Species: Other
Genetic Insert: CaMV 35S::dCas9
Vector Backbone Description: Vector Backbone:Binary; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239940 Copy
Species: Other
Genetic Insert: CaMV 35S-SynPro-01::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239946 Copy
Species: Other
Vector Backbone Description: Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None
Defining Citation: PMID:42199375
Comments: Genotype: E. coli B F- ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔendA ΔrecA glvC ::[ phlFAM, cymRAM, luxR, vanRAM, lacIAM, tetR ]
Fast growth at 37C in LB (doubling time ~ 20-25 minutes) and improved DNA (endA, recA) and protein stability (lon, ompT).
Strain validation:
- PCR using OR1965 (TGGCGTAAAGGTGCTTCC) and OR1960 (ATAACCAATCAGGCTTCCTACTTACAGAATTG) generates a product of 5 518 bp.
- PCR using OR1965 (TGGCGTAAAGGTGCTTCC) and OR1160(GCTTGTCGACGACGGC) generates a product of 140 bp.
- PCR using OR1157 (TACCCCAGTTGGGGCAC) and OR1960 (ATAACCAATCAGGCTTCCTACTTACAGAATTG) generates a product of 110 bp. Please visit https://doi.org/10.1101/2025.10.29.680610 for bioRxiv preprint.
Proper citation: RRID:Addgene_235121 Copy
Species: Other
Genetic Insert: CaMV 35S::dCas9
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239937 Copy
Species: Other
Genetic Insert: CaMV 35S::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239887 Copy
Species: Other
Genetic Insert: Hsp18.2::sgRNA-B
Vector Backbone Description: Vector Backbone:pCK2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38769424
Comments: Please note: This plasmid contains an IS4 transposable element inserted in the backbone. This insertion does not impact plasmid function.
Proper citation: RRID:Addgene_239936 Copy
Species: Other
Genetic Insert: mCherry fluorescent protein
Vector Backbone Description: Vector Backbone:pMAX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_241313 Copy
Species: Other
Genetic Insert: Pemax-T2A-GFP
Vector Backbone Description: Vector Backbone:n.a.; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40705858
Proper citation: RRID:Addgene_238541 Copy
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