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Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24803 Copy
Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24802 Copy
Genetic Insert: NpuDnaE-IntC
Vector Backbone Description: Backbone Size:3793; Vector Backbone:pRSF-1b (Invitrogen); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20203672
Proper citation: RRID:Addgene_24765 Copy
Species: Homo sapiens
Genetic Insert: TGFbeta receptor II
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: HA tag: PYDVPDYA
Proper citation: RRID:Addgene_24801 Copy
Species: Mus musculus
Genetic Insert: Zinc Finger Protein 423
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCV puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20200519
Proper citation: RRID:Addgene_24764 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18568018
Proper citation: RRID:Addgene_24814 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24749 Copy
Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA-4C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19553310
Proper citation: RRID:Addgene_24744 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24864 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24860 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24859 Copy
Species: Mus musculus
Genetic Insert: Ezh2
Vector Backbone Description: Backbone Size:7000; Vector Backbone:MSCV hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20368440
Proper citation: RRID:Addgene_24927 Copy
Species: Homo sapiens
Genetic Insert: p16
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBabe puro3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18394558
Proper citation: RRID:Addgene_24934 Copy
Species: Homo sapiens
Genetic Insert: HES1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Proper citation: RRID:Addgene_24982 Copy
Species: Homo sapiens
Genetic Insert: mir-9-3
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen, Addgene plasmid 11375; Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: The mir-9-3 gene was PCR-amplified from normal human genomic DNA and cloned into the MDH-PGK-GFP 2.0 retroviral vector. It includes the miR-9 stem-loop and ~100bp flanking sequences on both sides.
Proper citation: RRID:Addgene_25036 Copy
Species: Homo sapiens
Genetic Insert: CDH1 mutant 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector. The mutant construct of the CDH1 3′ UTR was generated with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
Proper citation: RRID:Addgene_25039 Copy
Species: Homo sapiens
Genetic Insert: STAT3
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19074878
Comments: A V667L mutation is present but not known to affect function.
Expression of EGFP is driven by the human ubiquitin promoter. See Addgene plasmid 14883: FUGW for more information on the empty vector.
Proper citation: RRID:Addgene_24983 Copy
Species: Homo sapiens
Genetic Insert: a mutant human MAP1-LC3B fused with HcRed
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCXN2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18000393
Proper citation: RRID:Addgene_24992 Copy
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Marker:Addgene Plasmid 11578; Backbone Size:6739; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20479239
Comments: pSico-Flpo allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html
Proper citation: RRID:Addgene_24969 Copy
Genetic Insert: pKW08-Lx-Int
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25015 Copy
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