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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMA_Level 1Γ Resource Report Resource Website |
RRID:Addgene_102703 | J23106:B0034:spisPink:rrnBT1-T7TE | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:26 | 0 | |||
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APPL1-N308D-mCherry Resource Report Resource Website |
RRID:Addgene_102784 | APPL1 | Homo sapiens | Kanamycin | *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function. | Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | changed asparagine 308 to aspartate; *(see below) | 2026-08-15 01:00:26 | 0 | |
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Rab5-L38R-mCherry Resource Report Resource Website |
RRID:Addgene_102785 | Rab5a | Homo sapiens | Kanamycin | mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame. | Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Leucine 38 changed to Arginine | 2026-08-15 01:00:26 | 0 | |
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pET_RTX_(exo-) Resource Report Resource Website 1+ mentions |
RRID:Addgene_102786 | reverse transcription xenopolymerase with 3'-5' deficient exonuclease | Other | Ampicillin | PMID:27339990 | Vector Backbone:modified pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:26 | 1 | ||
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hisSUMO-ZFP57 Resource Report Resource Website |
RRID:Addgene_102716 | Zinc finger protein 57 | Homo sapiens | Ampicillin | PMID:29159284 | Backbone Marker:NEB; Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:25 | 0 | ||
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HaloTag-bActinCDS-bActinUTR-MS2V5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_102718 | HaloTag-bActinCDS-bActin3'UTR-MS2V5 | Synthetic | Ampicillin | PMID:27791158 | Please note, due to the AT rich sequence within the Actin 3'UTR, there are some discrepancies between the Addgene NGS result and the provided full plasmid reference sequence. | Backbone Size:6250; Vector Backbone:pUbC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:25 | 7 | |
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pZH517 Resource Report Resource Website |
RRID:Addgene_102670 | GFPmut2 | Synthetic | Ampicillin | PMID:29084263 | Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint. | Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:25 | 0 | |
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pACYC-GFPM2 Resource Report Resource Website |
RRID:Addgene_102791 | Green Fluorescent Protein | Other | Chloramphenicol | PMID:29120463 | Backbone Marker:Novagen; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:00:26 | 0 | ||
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pACYC.GFP1Amb Resource Report Resource Website |
RRID:Addgene_102792 | Green Fluorescent Protein | Other | Chloramphenicol | PMID:29120463 | Backbone Marker:Novagen; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Y39Stop | 2026-08-15 01:00:26 | 0 | |
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pZH519 Resource Report Resource Website |
RRID:Addgene_102672 | GFPmut2 | Synthetic | Ampicillin | PMID:29084263 | Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint. | Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:25 | 0 | |
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pQE 80L Kan T7WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_102790 | T7 RNA polymerase | T7 phage | Kanamycin | PMID:29120463 | Backbone Marker:Qiagen; Vector Backbone:pQE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:26 | 1 | ||
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RF15 Resource Report Resource Website |
RRID:Addgene_102799 | none | None | PMID:33289521 | This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. Genotype= aspC tyrB trpA trpB glyA serB Precursor strain = RF14 Modified from the parent Escherichia coli BL21(DE3) strain Selective amino acid labeling (and/or requirement) = Asp, Tyr, Trp, (Phe), Gly, Ser+++++ +++++ RF15 has knockouts in aspC, tyrB, trpA, trpB, glyA and serB genes and requires the presence of L-Asp, L-Tyr, L-Trp, L-Gly plus L-Ser for growth in M63 minimal medium, but it does NOT grow in the presence of L-Asp, L-Tyr, L-Trp, L-Gly, L-Ser plus L-Cys (either in the presence or absence of L-Ala) (i.e., L-Cys inhibits the growth of RF15) Please visit the following links for additional details on this strain and selective amino acid labeling- http://www2.nms.ac.jp/fesworld/EcoliStrains.html http://www2.nms.ac.jp/fesworld/EcoliStrainsSuppl.html Note that these strains are NOT competent cells and one needs to make them competent before use. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. Supporting References: Lin, M. T., Fukazawa, R., Miyajima-Nakano, Y., Matsushita, S., Choi, S. K., Iwasaki, T., and Gennis, R. B. (2015) Escherichia coliauxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. (Isotope Labeling of Biomolecules - Labeling Methods), 565, 45-66. Iwasaki, T., Fukazawa, R., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M. T., Gennis, R. B., Hasegawa, K., Kumasaka, T., and Dikanov, S. A. (2012) Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134, 19731-19738. Lin, M. T., Sperling, L. J., Frericks Schmidt, H. L., Tang, M., Samoilova, R. I., Kumasaka, T., Iwasaki, T., Dikanov, S. A., Rienstra, C. M., and Gennis, R. B. (2011) A rapid and robust method for selective isotope labeling of proteins. Methods 55, 370-378. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:00:26 | 0 | ||
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pMA_Auxiliary 4Γ Resource Report Resource Website |
RRID:Addgene_102714 | Linker sequence | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:25 | 0 | |||
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pZH521 Resource Report Resource Website |
RRID:Addgene_102674 | GFPmut2 | Synthetic | Ampicillin | PMID:29084263 | Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint. | Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:25 | 0 | |
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pMIG-Flag-RIPK1-wt Resource Report Resource Website |
RRID:Addgene_102677 | RIPK1 | Homo sapiens | Ampicillin | PMID:26195820 | The RIPK1 insert has a K87Q polymorphism which has no effect on protein activity. | Backbone Size:7166; Vector Backbone:pMIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:25 | 0 | |
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pMA_Auxiliary 2 Resource Report Resource Website |
RRID:Addgene_102710 | Linker sequence | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:25 | 0 | |||
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Lv EF1a HP1cs-Frb1x PGK Puro Resource Report Resource Website |
RRID:Addgene_102808 | HP1cs-Frb1x | Ampicillin | PMID:28916764 | Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:26 | 0 | |||
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Lv EF1a HP1cs-Frb2x PGK Puro Resource Report Resource Website |
RRID:Addgene_102809 | HP1cs-Frb2x | Ampicillin | PMID:28916764 | Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:26 | 0 | |||
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RF21 Resource Report Resource Website |
RRID:Addgene_102803 | none | None | PMID:33289521 | This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. Genotype= aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) Precursor strain = RF18 Modified from the parent Escherichia coli BL21(DE3) strain Selective amino acid labeling (and/or requirement) = Asp, Tyr, Phe, Ile, Leu, Val#### #### RF21 has knockouts in the four general transaminase genes of E. coli (aspC, tyrB, ilvE, and avtA) and is found to require the presence of L-Asp, L-Tyr, L-Phe, L-Ile, L-Leu plus L-Val for slow growth in M63 minimal medium. Although RF21 strain has further knockouts in yfbQ (alaA) and yfdZ (alaC) genes, it is NOT an L-Ala auxotroph, either (requiring the presence of L-Asp, L-Tyr, L-Phe, L-Ile, L-Leu plus L-Val for slow growth in M63 minimal medium, like RF18). Please visit the following links for additional details on this strain and selective amino acid labeling- http://www2.nms.ac.jp/fesworld/EcoliStrains.html http://www2.nms.ac.jp/fesworld/EcoliStrainsSuppl.html Note that these strains are NOT competent cells and one needs to make them competent before use. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. Supporting References: Lin, M. T., Fukazawa, R., Miyajima-Nakano, Y., Matsushita, S., Choi, S. K., Iwasaki, T., and Gennis, R. B. (2015) Escherichia coliauxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. (Isotope Labeling of Biomolecules - Labeling Methods), 565, 45-66. Iwasaki, T., Fukazawa, R., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M. T., Gennis, R. B., Hasegawa, K., Kumasaka, T., and Dikanov, S. A. (2012) Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134, 19731-19738. Lin, M. T., Sperling, L. J., Frericks Schmidt, H. L., Tang, M., Samoilova, R. I., Kumasaka, T., Iwasaki, T., Dikanov, S. A., Rienstra, C. M., and Gennis, R. B. (2011) A rapid and robust method for selective isotope labeling of proteins. Methods 55, 370-378. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:00:26 | 0 | ||
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Lv EF1a MS2-Fkbp1x 2A Hygro Resource Report Resource Website |
RRID:Addgene_102806 | MS2-Fkbp | Ampicillin | PMID:28916764 | Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:26 | 0 |
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