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Species: Other
Genetic Insert: gRNA targeting NRF1-Ex7 splice site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237466 Copy
Species: Other
Genetic Insert: Intergenic sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237464 Copy
Species: Other
Genetic Insert: Intergenic sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237465 Copy
Species: Other
Genetic Insert: gRNA targeting NRF1-Ex7 splice site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pX459v2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41633991
Proper citation: RRID:Addgene_237468 Copy
Species: Other
Genetic Insert: Pemax-T2A-GFP
Vector Backbone Description: Vector Backbone:n.a.; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40705858
Proper citation: RRID:Addgene_238541 Copy
Species: Other
Genetic Insert: J23119 CAO1 target + CAO1 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253627 Copy
Species: Other
Genetic Insert: J23119 GFP T14 target + GFP T14 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253631 Copy
Species: Other
Genetic Insert: J23119 GFP T14 target + CAO1 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253632 Copy
Species: Other
Genetic Insert: IS1_TS1-A-PgpdA-uidA-TtrpC-B-IS1_TS2-ori-AmpR
Vector Backbone Description: Vector Backbone:pU2002; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.09.13.612835 for bioRxiv preprint.
Proper citation: RRID:Addgene_247963 Copy
Species: Other
Genetic Insert: Geo Sec Tag
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235442 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235444 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235446 Copy
Species: Other
Genetic Insert: Sp-gRNA
Vector Backbone Description: Vector Backbone:MLM3636; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226862 Copy
Species: Other
Genetic Insert: Lenti Sp-gRNA
Vector Backbone Description: Vector Backbone:pCRISPRia-v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Comments: BFP
Proper citation: RRID:Addgene_226867 Copy
Species: Other
Genetic Insert: dTomato
Vector Backbone Description: Vector Backbone:pdest Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39892392
Proper citation: RRID:Addgene_223054 Copy
Species: Other
Genetic Insert: DEXsynth:ccdb-eGFP + mcherry
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233312 Copy
Species: Other
Genetic Insert: Ubi:3xHA-ccdb + mcherry
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233301 Copy
Species: Other
Genetic Insert: photoswitchable HaloTag 1a variant
Vector Backbone Description: Backbone Marker:EMBL; Backbone Size:5334; Vector Backbone:pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41131894
Comments: Please visit https://doi.org/10.1101/2024.12.18.629107 for bioRxiv preprint.
Proper citation: RRID:Addgene_232940 Copy
Species: Other
Genetic Insert: DEXsynth:mTb-ccdb+ RUBY
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233321 Copy
Species: Other
Genetic Insert: DEXsynth:ccdb-3xHA + RUBY
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233319 Copy
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