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Species: Homo sapiens
Genetic Insert: HPV8 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24816794
Proper citation: RRID:Addgene_52598 Copy
Species: Homo sapiens
Genetic Insert: HPV43 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24816794
Proper citation: RRID:Addgene_52596 Copy
Species: Synthetic
Genetic Insert: Right AAVS1 TALEN
Vector Backbone Description: Vector Backbone:pZT; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24833591
Comments: pZT-AAVS1-R1 TALEN was assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT.
Proper citation: RRID:Addgene_52638 Copy
Species: Rattus norvegicus
Genetic Insert: Kir4.1
Vector Backbone Description: Vector Backbone:pZac 2.1; Vector Types:Mammalian Expression, AAV, Adeno-Associated Virus (); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24686787
Proper citation: RRID:Addgene_52874 Copy
Genetic Insert: hEµMAR-SFFV; eGFP
Vector Backbone Description: Backbone Size:6724; Vector Backbone:LeGO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23240650
Proper citation: RRID:Addgene_52633 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF15
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23863808
Proper citation: RRID:Addgene_52641 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF09
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23863808
Proper citation: RRID:Addgene_52640 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-RS09
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23863808
Proper citation: RRID:Addgene_52649 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-RS06
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23863808
Proper citation: RRID:Addgene_52647 Copy
Species: Discosoma
Genetic Insert: DsRed1-E5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3959; Vector Backbone:pDsRed2-Mito; Vector Types:DsRed; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24644293
Proper citation: RRID:Addgene_52659 Copy
Species: Synthetic
Genetic Insert: TEM-1 β-Lactamase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24817566
Proper citation: RRID:Addgene_52656 Copy
Genetic Insert: KI (37)-Oplac2 strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of Oplac2 with those of KIlac, producing and KI(37)-Oplac2.
Proper citation: RRID:Addgene_52704 Copy
Genetic Insert: degtag strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: To generate the degtag strain, we introduced the ssrA tag to the C-terminus of LacZ, thus targeting the protein for degradation by the ClpXP and ClpAP proteases.
Proper citation: RRID:Addgene_52705 Copy
Genetic Insert: KI (37)-Oplac1 strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of OpLac1 with those of KIlac.
Proper citation: RRID:Addgene_52703 Copy
Genetic Insert: OpLac2-Δ6 strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: Oplac2Δ6 was erroneously synthesized missing the first 6 nucleotides of Oplac2. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3.
Proper citation: RRID:Addgene_52700 Copy
Genetic Insert: Oplac1 (37)-KI strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of KILac with those of OpLac1.
Proper citation: RRID:Addgene_52701 Copy
Genetic Insert: S56L strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: Impaired LacY function
Proper citation: RRID:Addgene_52709 Copy
Genetic Insert: ΔZ strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: lacZ deletion
Proper citation: RRID:Addgene_52706 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pSILENCER; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
Proper citation: RRID:Addgene_52675 Copy
Species: Rattus norvegicus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4733; Vector Backbone:pTagRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: HindII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N. PSD-95 tyrosine 12 was mutated to glutamate (Y12E) by extending a complementary set of primers (PSD95Y12E-forward: 5’-GAC AAC CAA GAA AGA GCG CTA CCA AGA TGA AGA CAC G-3’, PSD95Y12E-reverse: 5'-CGT GTC TTC ATC TTG GTA GCG CTC TTT CTT GGT TGT C-3') along the whole template plasmid using Q5 high-fidelity DNA polymerase (NEB). DpnI was subsequently used to digest non-mutated, methylated template DNA prior to transformation.
Proper citation: RRID:Addgene_52672 Copy
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