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Species: Arabidopsis thaliana
Genetic Insert: AT4G28490
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLNQDATILRQAKLGLSDPAQSLSSWSDNNDVTPCKWLGVSCDATSNVVSVDLSSFMLVGPFPSILCHLPSLHSLSLYNNSINGSLSADDFDTCHNLISLDLSENLLVGSIPKSLPFNLPNLKFLEISGNNLSDTIPSSFGEFRKLESLNLAGNFLSGTIPASLGNVTTLKELKLAYNLFSPSQIPSQLGNLTELQVLWLAGCNLVGPIPPSLSRLTSLVNLDLTFNQLTGSIPSWITQLKTVEQIELFNNSFSGELPESMGNMTTLKRFDASMNKLTGKIPDNLNLLNLESLNLFENMLEGPLPESITRSKTLSELKLFNNRLTGVLPSQLGANSPLQYVDLSYNRFSGEIPANVCGEGKLEYLILIDNSFSGEISNNLGKCKSLTRVRLSNNKLSGQIPHGFWGLPRLSLLELSDNSFTGSIPKTIIGAKNLSNLRISKNRFSGSIPNEIGSLNGIIEISGAENDFSGEIPESLVKLKQLSRLDLSKNQLSGEIPRELRGWKNLNELNLANNHLSGEIPKEVGILPVLNYLDLSSNQFSGEIPLELQNLKLNVLNLSYNHLSGKIPPLYANKIYAHDFIGNPGLCVDLDGLCRKITRS
Proper citation: RRID:Addgene_115108 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G49670
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SFTVAKPITELHALLSLKSSFTIDEHSPLLTSWNLSTTFCSWTGVTCDVSLRHVTSLDLSGLNLSGTLSSDVAHLPLLQNLSLAANQISGPIPPQISNLYELRHLNLSNNVFNGSFPDELSSGLVNLRVLDLYNNNLTGDLPVSLTNLTQLRHLHLGGNYFSGKIPATYGTWPVLEYLAVSGNELTGKIPPEIGNLTTLRELYIGYYNAFENGLPPEIGNLSELVRFDAANCGLTGEIPPEIGKLQKLDTLFLQVNAFTGTITQELGLISSLKSMDLSNNMFTGEIPTSFSQLKNLTLLNLFRNKLYGAIPEFIGEMPELEVLQLWENNFTGSIPQKLGENGRLVILDLSSNKLTGTLPPNMCSGNRLMTLITLGNFLFGSIPDSLGKCESLTRIRMGENFLNGSIPKELFGLPKLSQVELQDNYLTGELPISGGGVSGDLGQISLSNNQLSGSLPAAIGNLSGVQKLLLDGNKFSGSIPPEIGRLQQLSKLDFSHNLFSGRIAPEISRCKLLTFVDLSRNELSGDIPNELTGMKILNYLNLSRNHLVGSIPVTIASMQSLTSVDFSYNNLSGLVPSTGQFSYFNYTSFVGNSHLCGPYLGPCGKGTHQSHVKPLS
Proper citation: RRID:Addgene_115104 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT4G20270
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLISPLNLSLIRQANVLISLKQSFDSYDPSLDSWNIPNFNSLCSWTGVSCDNLNQSITRLDLSNLNISGTISPEISRLSPSLVFLDISSNSFSGELPKEIYELSGLEVLNISSNVFEGELETRGFSQMTQLVTLDAYDNSFNGSLPLSLTTLTRLEHLDLGGNYFDGEIPRSYGSFLSLKFLSLSGNDLRGRIPNELANITTLVQLYLGYYNDYRGGIPADFGRLINLVHLDLANCSLKGSIPAELGNLKNLEVLFLQTNELTGSVPRELGNMTSLKTLDLSNNFLEGEIPLELSGLQKLQLFNLFFNRLHGEIPEFVSELPDLQILKLWHNNFTGKIPSKLGSNGNLIEIDLSTNKLTGLIPESLCFGRRLKILILFNNFLFGPLPEDLGQCEPLWRFRLGQNFLTSKLPKGLIYLPNLSLLELQNNFLTGEIPEEEAGNAQFSSLTQINLSNNRLSGPIPGSIRNLRSLQILLLGANRLSGQIPGEIGSLKSLLKIDMSRNNFSGKFPPEFGDCMSLTYLDLSHNQISGQIPVQISQIRILNYLNVSWNSFNQSLPNELGYMKSLTSADFSHNNFSGSVPTSGQFSYFNNTSFLGNPFLCGFSSNPCNGSQNQSQSQLLNQNNARSRGEIS
Proper citation: RRID:Addgene_115106 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT4G26540
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLDQQGQALLSWKSQLNISGDAFSSWHVADTSPCNWVGVKCNRRGEVSEIQLKGMDLQGSLPVTSLRSLKSLTSLTLSSLNLTGVIPKEIGDFTELELLDLSDNSLSGDIPVEIFRLKKLKTLSLNTNNLEGHIPMEIGNLSGLVELMLFDNKLSGEIPRSIGELKNLQVLRAGGNKNLRGELPWEIGNCENLVMLGLAETSLSGKLPASIGNLKRVQTIAIYTSLLSGPIPDEIGYCTELQNLYLYQNSISGSIPTTIGGLKKLQSLLLWQNNLVGKIPTELGNCPELWLIDFSENLLTGTIPRSFGKLENLQELQLSVNQISGTIPEELTNCTKLTHLEIDNNLITGEIPSLMSNLRSLTMFFAWQNKLTGNIPQSLSQCRELQAIDLSYNSLSGSIPKEIFGLRNLTKLLLLSNDLSGFIPPDIGNCTNLYRLRLNGNRLAGSIPSEIGNLKNLNFVDISENRLVGSIPPAISGCESLEFLDLHTNSLSGSLLGTTLPKSLKFIDFSDNALSSTLPPGIGLLTELTKLNLAKNRLSGEIPREISTCRSLQLLNLGENDFSGEIPDELGQIPSLAISLNLSCNRFVGEIPSRFSDLKNLGVLDVSHNQLTGNLNVLTDLQNLVSLNISYNDFSGDLPNTPFFRRLPLSDLASNRGLYISNAISTRPDPTTRNS
Proper citation: RRID:Addgene_115107 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G24650
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SPDEAVMIALRDSLKLSGNPNWSGSDPCKWSMFIKCDASNRVTAIQIGDRGISGKLPPDLGKLTSLTKFEVMRNRLTGPIPSLAGLKSLVTVYANDNDFTSVPEDFFSGLSSLQHVSLDNNPFDSWVIPPSLENATSLVDFSAVNCNLSGKIPDYLFEGKDFSSLTTLKLSYNSLVCEFPMNFSDSRVQVLMLNGQKGREKLHGSISFLQKMTSLTNVTLQGNSFSGPLPDFSGLVSLKSFNVRENQLSGLVPSSLFELQSLSDVALGNNLLQGPTPNFTAPDIKPDLNGLNSFCLDTPGTSCDPRVNTLLSIVEAFGYPVNFAEKWKGNDPCSGWVGITCTGTDITVINFKNLGLNGTISPRFADFASLRVINLSQNNLNGTIPQELAKLSNLKTLDVSKNRLCGEVPRFNTTIVNTTGNFEDCPNGNAGKKASS
Proper citation: RRID:Addgene_115070 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G66150
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SDGDLSAMLSLKKSLNPPSSFGWSDPDPCKWTHIVCTGTKRVTRIQIGHSGLQGTLSPDLRNLSELERLELQWNNISGPVPSLSGLASLQVLMLSNNNFDSIPSDVFQGLTSLQSVEIDNNPFKSWEIPESLRNASALQNFSANSANVSGSLPGFLGPDEFPGLSILHLAFNNLEGELPMSLAGSQVQSLWLNGQKLTGDITVLQNMTGLKEVWLHSNKFSGPLPDFSGLKELESLSLRDNSFTGPVPASLLSLESLKVVNLTNNHLQGPVPVFKSSVSVDLDKDSNSFCLSSPGECDPRVKSLLLIASSFDYPPRLAESWKGNDPCTNWIGIACSNGNITVISLEKMELTGTISPEFGAIKSLQRIILGINNLTGMIPQELTTLPNLKTLDVSSNKLFGKVPGFRSNVVVNTNGNPDIGKDKSSLSSPGSSSPSGGSGSGINGDKDRRGMKS
Proper citation: RRID:Addgene_115071 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 46Q glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11515 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G56120
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SIFAAWKIRAPREWNISGELCSGVAIDASVLDSNHAYNPLIKCDCSFQNSTICRINNIKVYAIDVVGPIPPELWTLTYLTNLNLGQNYLTGSLSPAIGNLTRMQWMTFGINALSGPIPKEIGLLTDLRLLGISSNNFSGSLPAEIGSCTKLQQMYIDSSGLSGGIPLSFANFVELEVAWIMDVELTGRIPDFIGFWTKLTTLRILGTGLSGPIPSSFSNLIALTELRLGDISNGSSSLDFIKDMKSLSVLVLRNNNLTGTIPSTIGGYTSLQQVDLSFNKLHGPIPASLFNLSRLTHLFLGNNTLNGSLPTLKGQSLSNLDVSYNDLSGSLPSWVSLPDLKLNLVANNFTLEGLDNRVLSGLHCLQKNFPCNRGEGIYYNFSINCGGPEIRSVSGALFEKEDADLGPASFVVSAAKRWAASSVGNFAGSSNNIYIATSLAQFINTMDSELFQSARLSASSLRYYGLGLENGGYTVTLQFAEVQIEGSNSWKGIGRRRFNIYVQGRLVEKDFDIRRTAGGSSVRAVQREYKTNVSENHLEVHLFWAGKGTCCIPIQGAYGPLIAAVSATPDFTPTVANRPPSKGKS
Proper citation: RRID:Addgene_115060 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G56130
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: QTGATTHPDEARALNSIFAAWKIQAPREWNISGELCSGAAIDASVLDSNPAYNPLIKCDCSFQNSTICRITNIKVYAIDVVGPIPPELWTLTYLTNLNLGQNVLTGSLPPAIGNLTRMQWMTFGINALSGPVPKEIGLLTDLRLLGISSNNFSGSIPDEIGRCTKLQQMYIDSSGLSGRIPLSFANLVQLEQAWIADLEVTDQIPDFIGDWTKLTTLRIIGTGLSGPIPSSFSNLTSLTELRLGDISSGSSSLDFIKDMKSLSVLVLRNNNLTGTIPSTIGEHSSLRQVDLSFNKLHGPIPASLFNLSQLTHLFLGNNTLNGSFPTQKTQSLRNVDVSYNDLSGSLPSWVSLPSLKLNLVANNFTLEGLDNRVLPGLNCLQKNFPCNRGKGIYSDFSINCGGPEKRSVTGALFEREDEDFGPASFFVSAGQRWAASSVGLFAGSSNNIYIATSQSQFVNTLDSELFQSARLSASSVRYYGLGLENGGYTVTLQFAEIQILGSTSTTWKGLGRRRFDIYVQGRLVEKDFDVRRTAGDSTVRAVQRVYKANVSENHLEVHLFWAGKGTCCIPIQGAYGPLISAVSATPDFTPTVANKPPSKGKNRT
Proper citation: RRID:Addgene_115061 Copy
Species: Synthetic
Genetic Insert: mW attP-CC FRT
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pHD-DsRed-attP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30065043
Comments: Based on pHD-DsRed-attP vector (Gratz et al., Genetics, 2014; DOI: 10.1534/genetics.113.160713)
Proper citation: RRID:Addgene_115158 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 25 glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11508 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115144 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115145 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9-T2A-BlastR
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115146 Copy
Species: Synthetic
Genetic Insert: human codon optimized Orf2mor anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115141 Copy
Species: Synthetic
Genetic Insert: human codon optimized Mb3Cas12a
Vector Backbone Description: Backbone Marker:Cepko lab (Addgene Plasmid #11179); Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115142 Copy
Species: Synthetic
Genetic Insert: BlastR-P2A-dCas9-VPR
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115148 Copy
Species: Homo sapiens
Genetic Insert: Safe Harbor Homology Sequences
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:4944; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115149 Copy
Species: Streptococcus pyogenes
Genetic Insert: Dual SH231 gRNAs
Vector Backbone Description: Backbone Size:2739; Vector Backbone:custom; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115150 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G02130
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: KITVLADSDKSVLLRFKKTVSDPGSILASWVEESEDYCSWFGVSCDSSSRVMALNISGSGSSEISRNRFTCGDIGKFPLYGFGVRRDCTGNHGALAGNLPSVIMSLTGLRVLSLPFNSFSGEIPVGIWGMEKLEVLDLEGNLMTGSLPDQFTGLRNLRVMNLGFNRVSGEIPNSLQNLTKLEILNLGGNKLNGTVPGFVGRFRVLHLPLNWLQGSLPKDIGDSCGKLEHLDLSGNFLTGRIPESLGKCAGLRSLLLYMNTLEETIPLEFGSLQKLEVLDVSRNTLSGPLPVELGNCSSLSVLVLSNLYNVYEDINSVRGEADLPPGADLTSMTEDFNFYQGGIPEEITRLPKLKILWVPRATLEGRFPGDWGSCQNLEMVNLGQNFFKGEIPVGLSKCKNLRLLDLSSNRLTGELLKEISVPCMSVFDVGGNSLSGVIPDFLNNTTSHCPPVVYFDRFSIESYSDPSSVYLSFFTEKAQVGTSLIDLGSDGGPAVFHNFADNNFTGTLKSIPLAQERLGKRVSYIFSAGGNRLYGQFPGNLFDNCDELKAVYVNVSFNKLSGRIPQGLNNMCTSLKILDASVNQIFGPIPTSLGDLASLVALNLSWNQLQGQIPGSLGKKMAALTYLSIANNNLTGQIPQSFGQLHSLDVLDLSSNHLSGGIPHDFVNLKNLTVLLLNNNNLSGPIPSGFATFAVFNVSSNNLSGPVPSTNGLTKCSTVSGNPYLRPCHVFSLTTPSSDSRDSTGDSITQDYASSPVENAPSQSPGKGGFNS
Proper citation: RRID:Addgene_115135 Copy
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