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Species: Homo sapiens
Genetic Insert: photoactivatable Rac1
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory at bp# 895, 920 and 1702. According to the depositing lab, these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_41878 Copy
Species: Homo sapiens
Genetic Insert: CUL1
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21963094
Proper citation: RRID:Addgene_41911 Copy
Species: Homo sapiens
Genetic Insert: CUL4B
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21963094
Proper citation: RRID:Addgene_41915 Copy
Genetic Insert: U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Vector Backbone Description: Backbone Size:7732; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23287718
Comments: Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
If you ordered or downloaded sequence of this vector prior to June 17, 2013, please note that while the plasmid you received is correct, the earlier version of vector sequence provided for this construct misses a small portion (~2kb) outside the insert, and we have now updated it with a complete version.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_41863 Copy
Species: Homo sapiens
Genetic Insert: ATR ATR serine/threonine kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11721054
Proper citation: RRID:Addgene_41909 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:9364; Vector Backbone:pCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23193063
Proper citation: RRID:Addgene_41858 Copy
Species: Mus musculus
Genetic Insert: Trp53
Vector Backbone Description: Backbone Size:9364; Vector Backbone:pCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23193063
Proper citation: RRID:Addgene_41856 Copy
Species: Homo sapiens
Genetic Insert: TNRC6B
Vector Backbone Description: Backbone Size:5338; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150874
Proper citation: RRID:Addgene_42022 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pVL1393; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18782620
Proper citation: RRID:Addgene_41850 Copy
Species: Synthetic
Genetic Insert: HYPER 3
Vector Backbone Description: Backbone Size:3425; Vector Backbone:pQE-30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23256573
Proper citation: RRID:Addgene_42132 Copy
Species: Synthetic
Genetic Insert: mCherry-mVenus
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23654270
Proper citation: RRID:Addgene_42133 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22205962
Proper citation: RRID:Addgene_42081 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22205962
Proper citation: RRID:Addgene_42082 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22205962
Proper citation: RRID:Addgene_42083 Copy
Species: Drosophila melanogaster
Genetic Insert: scribble
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688207
Proper citation: RRID:Addgene_42064 Copy
Genetic Insert: DsRed shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6348; Vector Backbone:pSuper.Retro.Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150874
Proper citation: RRID:Addgene_42053 Copy
Species: Synthetic
Genetic Insert: RT-cassette
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:7904; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition enabling stable propagation of RT-cassette. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42150 Copy
Species: Homo sapiens
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP-TOPO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21668996
Proper citation: RRID:Addgene_42142 Copy
Species: Mus musculus
Genetic Insert: Frizzled 7
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23095888
Proper citation: RRID:Addgene_42259 Copy
Genetic Insert: Streptococcus pyogenes Cas9-3X Flag
Vector Backbone Description: Vector Backbone:unknown; Vector Types:CRISPR, zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23360964
Comments: Plasmid Features:
CAG enhancer-CMV promoter = 7466-370
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
BGHpA = 4612-4839
Proper citation: RRID:Addgene_42252 Copy
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