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Species: Caenorhabditis elegans
Genetic Insert: RGG-GFP-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124930 Copy
Species: Caenorhabditis elegans
Genetic Insert: RGG-RFP-RGG (using LAF-1 RGG domain)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061688
Proper citation: RRID:Addgene_124934 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S-KVS-1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19850480
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113062 Copy
Species: Caenorhabditis elegans
Genetic Insert: K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16222231
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113061 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1.2
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Comments: This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
Proper citation: RRID:Addgene_113068 Copy
Species: Caenorhabditis elegans
Genetic Insert: MiRP K+ channel accessory Subunit 3
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15799965
Comments: This plasmid contains a T61A mutation compared to the reference NP_490778.1 that does not impact function.
Proper citation: RRID:Addgene_113071 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Proper citation: RRID:Addgene_113070 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: Full length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11337 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
Please note that Addgene's sequencing result differs from GenBank ID NM_067348, which indicates mutations in nud-1 are present at K136R, C220G and E235G.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11339 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11338 Copy
Species: Caenorhabditis elegans
Genetic Insert: 56-2741: odr-3 promoter
Vector Backbone Description: Backbone Marker:Andy Fire; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20220099
Proper citation: RRID:Addgene_100897 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP^SEC^AID*::3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Comments: This plasmid is derived from pDD282 (AddGene plasmid 66823)
Proper citation: RRID:Addgene_121054 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-T^SEC^AID*::3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Comments: This plasmid is derived from pDD285 (AddGene plasmid 66826)
Proper citation: RRID:Addgene_121057 Copy
Species: Caenorhabditis elegans
Genetic Insert: dendra2 (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139035 Copy
Species: Caenorhabditis elegans
Genetic Insert: mkate2 (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139032 Copy
Species: Caenorhabditis elegans
Genetic Insert: epdz (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139033 Copy
Species: Caenorhabditis elegans
Genetic Insert: cox-4 (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139038 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm-20 (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139036 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm-20 (1-55 a.a.) (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139037 Copy
Species: Caenorhabditis elegans
Genetic Insert: mscarlet (includes STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139042 Copy
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