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Plasmids are provided by Addgene and DGRC.

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On page 32 showing 621 ~ 640 out of 644 results
Snippet view Table view Download 644 Result(s)
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http://www.addgene.org/46277

Species: Gallus gallus
Genetic Insert: ECFP + Vcl 1-419
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15883197
Comments: To make the control FRET probe pEYFP-C1/CFPV1-400, a HindIII site was engineered before the ATG site of pET15b/CFP-V1-851 and a KpnI site after codon 400 by PCR amplification with 5 -CAAGCTTCGatggtgagcaagggc-3 and 5 -GGTACCTCAtgcaactttccttgc-3 . The PCR product was introduced into TOPO pCRII and sequenced. The HindIII–KpnI fragment of CFP-vinculin1-400 was subcloned into pEYFP-C1 to generate the control plasmid pEYFP-CFPV1-400. Tandem EYFP-ECFP- hooked to portion of vinculin that localizes to FA; maximum FRET.

Proper citation: RRID:Addgene_46277 Copy   


http://www.addgene.org/46278

Species: Gallus gallus
Genetic Insert: Vcl 1-1066
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15883197

Proper citation: RRID:Addgene_46278 Copy   


http://www.addgene.org/46276

Species: Gallus gallus
Genetic Insert: Vcl 1-1066 + EYFP
Vector Backbone Description: Backbone Marker:Craig lab; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15883197
Comments: A 9-bp fragment encoding a NotI site was introduced by mutagenesis into pEGFP-C1/vinculin cDNA (chicken) immediately after the codon for aa 883 (Coutu and Craig, 1988) using the Quick Change kit (Stratagene). The cDNA of EYFP (CLONTECH Laboratories, Inc.) minus the stop codon and flanked by NotI was inserted into NotI-digested vinculin.

Proper citation: RRID:Addgene_46276 Copy   


  • RRID:Addgene_46268

    This resource has 1+ mentions.

http://www.addgene.org/46268

Species: Gallus gallus
Genetic Insert: Vcl 1-851
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16608855
Comments: Nde-Xho fragment from pET15b/Vh1-851 (Addgene plasmid 46172) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site.

Proper citation: RRID:Addgene_46268 Copy   


http://www.addgene.org/179484

Species: Gallus gallus
Genetic Insert: paxillin-Electra1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3933; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35715437
Comments: Excitation:403nm, Emission:456nm

Proper citation: RRID:Addgene_179484 Copy   


  • RRID:Addgene_92394

    This resource has 1+ mentions.

http://www.addgene.org/92394

Species: Gallus gallus
Genetic Insert: chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
Vector Backbone Description: Backbone Size:3055; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29386245
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.

Proper citation: RRID:Addgene_92394 Copy   


  • RRID:Addgene_245897

http://www.addgene.org/245897

Species: Gallus gallus
Genetic Insert: VinTS
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Site-directed mutagenesis was used to install I12K mutation onto the VinTS-A50I vector (Addgene 111827). Please visit https://doi.org/10.64898/2026.05.07.722930 for bioRxiv preprint.

Proper citation: RRID:Addgene_245897 Copy   


http://www.addgene.org/245898

Species: Gallus gallus
Genetic Insert: VinTS
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Site-directed mutagenesis was used to install I12K mutation onto the VinTS-A50I/I997A vector (Addgene 111829). Please visit https://doi.org/10.64898/2026.05.07.722930 for bioRxiv preprint.

Proper citation: RRID:Addgene_245898 Copy   


  • RRID:Addgene_250721

http://www.addgene.org/250721

Species: Gallus gallus
Genetic Insert: Chicken RPE65 E148Q
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_250721 Copy   


  • RRID:Addgene_250720

http://www.addgene.org/250720

Species: Gallus gallus
Genetic Insert: Chicken RPE65
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_250720 Copy   


http://www.addgene.org/250152

Species: Gallus gallus
Genetic Insert: pAce-Kv
Vector Backbone Description: Backbone Size:5599; Vector Backbone:pAAV-EF1a; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40987870

Proper citation: RRID:Addgene_250152 Copy   


http://www.addgene.org/225571

Species: Gallus gallus
Genetic Insert: hen egg lysozyme
Vector Backbone Description: Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.

Proper citation: RRID:Addgene_225571 Copy   


http://www.addgene.org/225214

Species: Gallus gallus
Genetic Insert: HEL
Vector Backbone Description: Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.

Proper citation: RRID:Addgene_225214 Copy   


http://www.addgene.org/225581

Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Naldini lab; Vector Backbone:p1074.NGFR.mCMV.hPGK.GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.

Proper citation: RRID:Addgene_225581 Copy   


http://www.addgene.org/216761

Species: Gallus gallus
Genetic Insert: mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
Vector Backbone Description: Backbone Size:7600; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38973210
Comments: This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. Requires co-transfection with transposase

Proper citation: RRID:Addgene_216761 Copy   


http://www.addgene.org/216763

Species: Gallus gallus
Genetic Insert: mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
Vector Backbone Description: Backbone Size:7600; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38973210
Comments: This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. PILATeS is appended C-terminally to vinculin as an unloaded negative control. Requires co-transfection with transposase

Proper citation: RRID:Addgene_216763 Copy   


  • RRID:Addgene_64599

    This resource has 10+ mentions.

http://www.addgene.org/64599

Species: Gallus gallus
Genetic Insert: Ovalbumin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11313828

Proper citation: RRID:Addgene_64599 Copy   


  • RRID:Addgene_240417

http://www.addgene.org/240417

Species: Gallus gallus
Genetic Insert: H2B
Vector Backbone Description: Vector Backbone:pHREP-KD-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41666149

Proper citation: RRID:Addgene_240417 Copy   


  • RRID:Addgene_240419

http://www.addgene.org/240419

Species: Gallus gallus
Genetic Insert: H2B
Vector Backbone Description: Vector Backbone:pHREP-RE-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41666149

Proper citation: RRID:Addgene_240419 Copy   


  • RRID:Addgene_241994

http://www.addgene.org/241994

Species: Gallus gallus
Genetic Insert: H1
Vector Backbone Description: Vector Backbone:pHREP-KD-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41666149

Proper citation: RRID:Addgene_241994 Copy   



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