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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pPSSUB-101 Resource Report Resource Website |
RRID:Addgene_26580 | destabilized GFP | Aequorea victoria | Chloramphenicol | PMID:15096621 | A novel hybrid promoter luxPRcI-OR1 (wild-type luxPR promoter with a CI OR1 operator site inserted at the +1 transcription start) drives expression of GFP(LVA). The promoter was constructed by encoding OR1 on PCR primers and inserting it after luxPR | Backbone Size:0; Vector Backbone:pLuxI-tet8 + pRKM-102; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | S2R, C-terminal extension RPAANDENYA(LVA) | 2026-08-15 01:12:54 | 0 |
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pBB528 Resource Report Resource Website |
RRID:Addgene_27390 | lacIq | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:4945; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:13:02 | 0 | |
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pDE43-MCSq22 Resource Report Resource Website |
RRID:Addgene_27335 | qtag22 | Chloramphenicol | PMID:21203517 | Backbone Marker:N/A; Backbone Size:4522; Vector Backbone:pTCS-2M1-icl2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | N/A | 2026-08-15 01:13:02 | 0 | ||
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pDE43-MCSq17 Resource Report Resource Website |
RRID:Addgene_27333 | qtag17 | Chloramphenicol | PMID:21203517 | Backbone Marker:N/A; Backbone Size:4522; Vector Backbone:pTCS-2M1-icl2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | N/A | 2026-08-15 01:13:01 | 0 | ||
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pDE43-MCKq20 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27334 | qtag20 | Chloramphenicol | PMID:21203517 | Backbone Marker:N/A; Backbone Size:5740; Vector Backbone:pDE43-MCKq1; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol | N/A | 2026-08-15 01:13:02 | 1 | ||
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pBTB-3 Resource Report Resource Website |
RRID:Addgene_22819 | Chloramphenicol | PMID:16496398 | Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. | Backbone Size:3586; Vector Backbone:pBTB-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:15 | 0 | |||
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pCph8 Resource Report Resource Website |
RRID:Addgene_22869 | pCph8 | Synechocystis/E.coli fusion gene | Chloramphenicol | PMID:16306980 | This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759 | Backbone Marker:Clontech; Backbone Size:2021; Vector Backbone:pPROTet; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | First 517 amino acids of Cph1 and the last 229 amino acids of EnvZ. ILE TO VAL mutation at position 136 of the HK EnvZ domain. | 2026-08-15 01:12:16 | 0 |
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pBMTB-3 Resource Report Resource Website |
RRID:Addgene_22826 | Chloramphenicol | PMID:16496398 | Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. | Backbone Size:4831; Vector Backbone:pBMTB-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:15 | 0 | |||
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pBMT-3 Resource Report Resource Website |
RRID:Addgene_22838 | Chloramphenicol | PMID:16496398 | Backbone Size:3649; Vector Backbone:pBMT-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:15 | 0 | ||||
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pSEL1 Resource Report Resource Website |
RRID:Addgene_23011 | DHFR | Mus musculus | Chloramphenicol | PMID:19165721 | Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD/HisA & pBThybrid; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:17 | 0 | ||
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pSPK1 Resource Report Resource Website |
RRID:Addgene_18876 | polyphosphate kinase | E. coli | Chloramphenicol | PMID:9143103 | Backbone Size:0; Vector Backbone:pMMB206; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:40 | 0 | ||
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pDESTsmg Resource Report Resource Website |
RRID:Addgene_18878 | Chloramphenicol | PMID:17949993 | This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21). This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli. | Backbone Size:6573; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:40 | 0 | |||
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pAK02 Resource Report Resource Website |
RRID:Addgene_19001 | arabinose transporter araE under control of Ptac | E. coli | Chloramphenicol | PMID:11092865 | Backbone Size:0; Vector Backbone:pMMB207; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:41 | 0 | ||
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pAC-PHYT Resource Report Resource Website |
RRID:Addgene_19002 | crtE, crtB | E. herbicola | Chloramphenicol | PMID:11676567 | Backbone Marker:Cunningham et al., 1994; Backbone Size:0; Vector Backbone:pAC-LYC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:41 | 0 | ||
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9.5-Luc Resource Report Resource Website |
RRID:Addgene_21728 | p21 promoter deletion mutant | Homo sapiens | Chloramphenicol | PMID:8988173 | Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:04 | 0 | ||
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2-Luc-Del-50 Resource Report Resource Website |
RRID:Addgene_21730 | p21 promoter deletion mutant | Homo sapiens | Chloramphenicol | PMID:8988173 | Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol | Identical to 2-Luc except that the 50-bp region between -144 and -95 has been deleted. | 2026-08-15 01:12:04 | 0 | |
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pXDC61-FabI Resource Report Resource Website 1+ mentions |
RRID:Addgene_21842 | fabI | L. pneumophila | Chloramphenicol | PMID:19578436 | Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:06 | 1 | ||
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pBAD33-mf-lon Resource Report Resource Website 1+ mentions |
RRID:Addgene_21867 | Lon | Mesoplasma florum | Chloramphenicol | PMID:18852454 | A detailed protocol for purification of mf-lon can be found in the associated paper. | Backbone Marker:Beckwith Lab; Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | all TGA codons changed to TGG codons | 2026-08-15 01:12:06 | 2 |
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pKOV Resource Report Resource Website 10+ mentions |
RRID:Addgene_25769 | Chloramphenicol | PMID:9335267 | pKOV is identical to the pKO3 vector described in J. Bacteriology 179: 6228-6237, except for the addition of a 3kb stuffer sequence in the multiple cloning site. This stuffer permits (i) directional cloning using NotI and BamHI and (ii) clean separation of doubly cut vector from singly cut contaminants when using this pair of enzymes. The pKOV cloning site is: 5' - SmaI - NotI - SmaI- stuffer - BamHI - SalI - 3'. BamHI and SalI are not used together, nor is SmaI used with NotI. BglII & BclI cut ends are compatible with BamHI. PmeI & SwaI are compatible with SmaI. NOTE: pKOV has a temperature sensitive pSC101 replication origin. To recover the plasmid, strains harboring the plasmid must be grown at 30 deg C under chloramphenicol selection. Gene replacement: Mutant alleles cloned into the pKOV gene replacement vector are electroporated into recombination proficient strains (eg. EMG2) and allowed to recover for 1 h at 30 deg C. The cells are plated on prewarmed chloramphenicol/LB plates and incubated at 42 deg C. To measure the integration frequency, the electroporated cells are also plated on chloramphenicol/LB plates at 30 deg C. From the 42 deg C plate, 1-5 colonies are picked into 1 ml of LB broth, serially diluted, and immediately plated at 30°ree;C on either 5% w/v sucrose or 5% sucrose+antibiotic plates. The 5% sucrose plates are replica plated to chloramphenicol plates at 30 deg C to test for loss of the replacement vector (cms). The gene replacement is confirmed by either PCR using primers flanking the targeted open reading frame or by genomic Southern's. Note from depositor: If sucrose plates do not select correctly, pKOV should be used in liquid selection on a plate reader with a LB + Cm control growth and a LB + Cm + sucrose growth. pKOV can sometimes still allow survival in the presence of sucrose, but significantly decreases fitness, so tracking the kinetic growth can be helpful. The sacB gene loses its efficacy,, sacB is toxic in E. coli even in the absence of sucrose. | Backbone Marker:N/A; Backbone Size:8673; Vector Backbone:pMAK700, pMAK705, pBS-TS; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:47 | 10 | |||
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pBTBXh-3 Resource Report Resource Website |
RRID:Addgene_26079 | Chloramphenicol | PMID:20148414 | There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function. | Backbone Size:3691; Vector Backbone:pBTBXh-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:48 | 0 |
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