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  • Organism:gallus gallus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET
 
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RRID:Addgene_46277 ECFP + Vcl 1-419 Gallus gallus Kanamycin PMID:15883197 To make the control FRET probe pEYFP-C1/CFPV1-400, a HindIII site was engineered before the ATG site of pET15b/CFP-V1-851 and a KpnI site after codon 400 by PCR amplification with 5 -CAAGCTTCGatggtgagcaagggc-3 and 5 -GGTACCTCAtgcaactttccttgc-3 . The PCR product was introduced into TOPO pCRII and sequenced. The HindIII–KpnI fragment of CFP-vinculin1-400 was subcloned into pEYFP-C1 to generate the control plasmid pEYFP-CFPV1-400. Tandem EYFP-ECFP- hooked to portion of vinculin that localizes to FA; maximum FRET. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin aa 1-419 2026-08-15 01:15:30 0
pEYFPN3/GgVcl 1-1066 (YFP at C term)
 
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RRID:Addgene_46278 Vcl 1-1066 Gallus gallus Kanamycin PMID:15883197 Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:30 0
GgVcl 1-1066 FRET tail probe 1
 
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RRID:Addgene_46276 Vcl 1-1066 + EYFP Gallus gallus Kanamycin PMID:15883197 A 9-bp fragment encoding a NotI site was introduced by mutagenesis into pEGFP-C1/vinculin cDNA (chicken) immediately after the codon for aa 883 (Coutu and Craig, 1988) using the Quick Change kit (Stratagene). The cDNA of EYFP (CLONTECH Laboratories, Inc.) minus the stop codon and flanked by NotI was inserted into NotI-digested vinculin. Backbone Marker:Craig lab; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin addition of 9 bp linker and EYFP at aa 883 2026-08-15 01:15:30 0
pEGFPC1/GgVcl 1-851 (Vh)
 
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1+ mentions
RRID:Addgene_46268 Vcl 1-851 Gallus gallus Kanamycin PMID:16608855 Nde-Xho fragment from pET15b/Vh1-851 (Addgene plasmid 46172) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 1-851 (Vh) head domain 2026-08-15 01:15:30 1
pPaxillin-Electra1-N1
 
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RRID:Addgene_179484 paxillin-Electra1 Gallus gallus Kanamycin PMID:35715437 Excitation:403nm, Emission:456nm Backbone Marker:Clontech; Backbone Size:3933; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:41 0
pcU6_3 MS2 sgRNA
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_92394 chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops Gallus gallus Tetracycline PMID:29386245 Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint. Backbone Size:3055; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline 2026-08-15 01:22:30 1
VinTS_I12KA50I
 
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RRID:Addgene_245897 VinTS Gallus gallus Ampicillin Site-directed mutagenesis was used to install I12K mutation onto the VinTS-A50I vector (Addgene 111827). Please visit https://doi.org/10.64898/2026.05.07.722930 for bioRxiv preprint. Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin I12K, A50I 2026-08-15 01:35:58 0
VinTS_I12K/A50I/I997A
 
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RRID:Addgene_245898 VinTS Gallus gallus Ampicillin Site-directed mutagenesis was used to install I12K mutation onto the VinTS-A50I/I997A vector (Addgene 111829). Please visit https://doi.org/10.64898/2026.05.07.722930 for bioRxiv preprint. Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin I12K, A50I, I997A 2026-08-15 01:35:58 0
GgRPE65_E148Q
 
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RRID:Addgene_250721 Chicken RPE65 E148Q Gallus gallus Ampicillin Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin C318>T, G606>A, T963>C, C1156>T, C1161>T, G441>A, G442>C 2026-08-15 01:36:04 0
GgRPE65
 
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RRID:Addgene_250720 Chicken RPE65 Gallus gallus Ampicillin Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin C318>T, G606>A, T963>C, C1156>T, C1161>T 2026-08-15 01:36:04 0
pAAV-EF1a-DIO-pAce-Kv-WPRE
 
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RRID:Addgene_250152 pAce-Kv Gallus gallus Ampicillin PMID:40987870 Backbone Size:5599; Vector Backbone:pAAV-EF1a; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:36:26 0
p41.pA.mHELflag.mCMV.hPGK.CD63.GFP.WPRE
 
Resource Report
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RRID:Addgene_225571 hen egg lysozyme Gallus gallus Ampicillin Please visit https://ssrn.com/abstract=4912248 for preprint. Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:46 0
p60.pA.HEL.mCMV.hPGK.CD63GFP.wpre
 
Resource Report
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RRID:Addgene_225214 HEL Gallus gallus Ampicillin Please visit https://ssrn.com/abstract=4912248 for preprint. Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:46 0
p66.pA.NGFR.mCMV.hPGK.iOva.wpre
 
Resource Report
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RRID:Addgene_225581 OVA Gallus gallus Ampicillin Please visit https://ssrn.com/abstract=4912248 for preprint. Backbone Marker:Naldini lab; Vector Backbone:p1074.NGFR.mCMV.hPGK.GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:47 0
mCh-LgBiT-2A-EGFP-Vin-PILATeS
 
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RRID:Addgene_216761 mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS Gallus gallus Ampicillin PMID:38973210 This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. Requires co-transfection with transposase Backbone Size:7600; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:18 0
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
 
Resource Report
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RRID:Addgene_216763 mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT Gallus gallus Ampicillin PMID:38973210 This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. PILATeS is appended C-terminally to vinculin as an unloaded negative control. Requires co-transfection with transposase Backbone Size:7600; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:18 0
pcDNA3-OVA
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_64599 Ovalbumin Gallus gallus Ampicillin PMID:11313828 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:11 21
pHREP-KD-1-shH2B
 
Resource Report
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RRID:Addgene_240417 H2B Gallus gallus Ampicillin PMID:41666149 Vector Backbone:pHREP-KD-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:02 0
pHREP-RE-1-H2B
 
Resource Report
Resource Website
RRID:Addgene_240419 H2B Gallus gallus Ampicillin PMID:41666149 Vector Backbone:pHREP-RE-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:06 0
pHREP-KD-2-shH1
 
Resource Report
Resource Website
RRID:Addgene_241994 H1 Gallus gallus Ampicillin PMID:41666149 Vector Backbone:pHREP-KD-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:03 0

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