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Genetic Insert: B kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15583002
Comments: PKB substrate sequence: RKRDRLGTLGI.
Proper citation: RRID:Addgene_14877 Copy
Species: Tobacco Rattle Virus RNA 1
Genetic Insert: TRV RNA1
Vector Backbone Description: Backbone Size:11498; Vector Backbone:pBIN19; Vector Types:Plant vector with TRV RNA1; this vector used with pYL156(TRV RNA2) for virus induced gene silencing (VIGS); Bacterial Resistance:Kanamycin
Defining Citation: PMID:12028572
Proper citation: RRID:Addgene_148968 Copy
Species: Modified Tobacco Rattle Virus RNA 2
Genetic Insert: Modified TRV RNA2
Vector Backbone Description: Backbone Size:6426; Vector Backbone:pCAMBIA0390 T-DNA vector; Vector Types:T-DNA vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12028572
Comments: There are a few differences between the Addgene full sequence and the Genbank ID: AF406991. These discrepancies were confirmed to not cause functional differences:
- 1 bp insertion (T3313) in backbone.
- 1 bp mismatch (T5593C) and 1 bp insertion (G5646) in pVS1 RepA (known variant).
- IS4-like element inserted between ori and KanR (1338bp insert at positions 8295-9632bp).
Proper citation: RRID:Addgene_148969 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Naldini; Backbone Size:0; Vector Backbone:LV-pGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11854510
Comments: CAG promoter was cloned into the ClaI and BamHI sites of LV-pGFP, replacing the PGK promoter.
Proper citation: RRID:Addgene_14857 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294208
Comments: HIV vector with CMV promoter. Constructed by inserting the CMV-GFP fragment from pKS-CMV-GFP into the same sites of pHR'.
Proper citation: RRID:Addgene_14858 Copy
Vector Backbone Description: Backbone Size:7236; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.57, Ligation number NONE.
Proper citation: RRID:Addgene_1488 Copy
Species: Homo sapiens
Genetic Insert: miR-1
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:2600; Vector Backbone:pTRE-TIGHT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14697198
Comments: Expression of a transcript behind TRE-Tight tetracycline regulatable promoter. This vector is part of the BD-Tet-ON/Tet-Off system for inducible expression in mammalian cells.
miR-1 is predicted to target Glucose-6 phosphate 1-dehydrogenase (G6PD).
The sequence provided is approximated based on the information that miR-1 was inserted at the BamHI and ClaI sites of pTRE-TIGHT. There may be slight differences in the actual sequence near the cloning sites.
Proper citation: RRID:Addgene_14896 Copy
Species: Drosophila melanogaster
Genetic Insert: AlstR
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pAAV-Syn-IRES2-EGFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16846851
Comments: Plasmid features: Synapsin promoter 208-680, AlstR
1352-2536, IRES2 2570-3154, EGFP
3158-3895.
Proper citation: RRID:Addgene_14895 Copy
Species: evolved fluorescent protein
Genetic Insert: EBFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad/His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17444659
Proper citation: RRID:Addgene_14891 Copy
Species: S. stercoralis
Genetic Insert: strongyloides stercoralis G protein alpha subunit promoter
Vector Backbone Description: Backbone Size:4226; Vector Backbone:pAJ01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17945217
Comments: PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.
Proper citation: RRID:Addgene_14913 Copy
Species: Dictyostelium discoideum
Genetic Insert: enhanced monomeric red fluorescent protein
Vector Backbone Description: Backbone Size:4515; Vector Backbone:pAJ08; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17945217
Comments: PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.
Proper citation: RRID:Addgene_14918 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725255.1
Proper citation: RRID:Addgene_149258 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149270 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149271 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149268 Copy
Species: Bos taurus
Genetic Insert: PKA R1alpha regulatory subunit
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16214430
Proper citation: RRID:Addgene_14922 Copy
Species: E. coli
Genetic Insert: tTA2
Vector Backbone Description: Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38918382
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_149363 Copy
Species: Other
Genetic Insert: mem-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_149410 Copy
Species: Other
Genetic Insert: ER-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199915
Proper citation: RRID:Addgene_149415 Copy
Vector Backbone Description: Backbone Marker:Hellens et al, 2000; Backbone Size:3232; Vector Backbone:pGreen; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32409318
Proper citation: RRID:Addgene_149416 Copy
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