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Species: Mus musculus
Genetic Insert: TRIM30A
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26468522
Proper citation: RRID:Addgene_87081 Copy
Species: Mus musculus
Genetic Insert: TRIM30D
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26468522
Proper citation: RRID:Addgene_87084 Copy
Species: Mus musculus
Genetic Insert: Stat4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7545930
Comments: Diagnostic restriction digest with BamHI (3.1kb, 2.0kb, 1.9kb, and 0.9kb).
Proper citation: RRID:Addgene_8711 Copy
Species: Mus musculus
Genetic Insert: TBK1
Vector Backbone Description: Backbone Size:6890; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29445180
Proper citation: RRID:Addgene_87443 Copy
Species: Mus musculus
Genetic Insert: Bad
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11493700
Proper citation: RRID:Addgene_8778 Copy
Species: Mus musculus
Genetic Insert: Bcl-2
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pSFFV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8929531
Proper citation: RRID:Addgene_8776 Copy
Species: Mus musculus
Genetic Insert: Bid p22 wt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11099414
Proper citation: RRID:Addgene_8774 Copy
Species: Mus musculus
Genetic Insert: Stat3-C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10458605
Comments: Residues changed to Cys renders the molecule constitutively active. Diagnostic Restriction Digest with BamHI (3.2kb, 2.8kb, and 2.0kb). Due to a stop codon error made when tagging the Stat3 clone with FLAG (DYKDDDDK) additional residues precede (SR) and follow the tag (GPYSIVSPKC STOP). The ApaI cloning site is GGG CCC, which encodes the G and P residues coming after the FLAG tag. Therefore, if the insert is removed with a NotI and ApaI digestion, it will not have a stop codon included.
* E16K mutation found during QC
Proper citation: RRID:Addgene_8722 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha promoter dCRE
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12764228
Comments: 5' flanking sequence of mouse PGC-1 alpha gene PCR amplified between +78 and -2533 with respect to the transcriptional start site. Mutation of the CRE site between -146 and -129 from TGACGTCA to TAGATCTA.
NOTE: The full sequence from the depositor is theoretical. There are several discrepancies between Addgene's quality control sequence and the full sequence provided here, but those discrepancies do not appear to affect function. Several labs have published successfully with this plasmid.
Proper citation: RRID:Addgene_8888 Copy
Species: Mus musculus
Genetic Insert: SREBP-1c
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3160; Vector Backbone:pSV-Sport; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8336713
Comments: Note that this is a mouse SREBP-1c, while the paper reference is a rat SREBP-1c. SREBP-1c differs from SREBP1a over amino acids 3-5 and contains a deletion of amino acids 6-29. See Figure 1c of the associated publication for more information.
Addgene's sequencing results identified a deletion of aa90-131 of SREBP1c compared to GenBank sequence NP_035610.1.
Proper citation: RRID:Addgene_8883 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12764228
Comments: 5' flanking sequence of mouse PGC-1 alpha gene PCR amplified between +78 and -2533 with respect to the transcriptional start site.
Proper citation: RRID:Addgene_8887 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX Gal4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10558993
Comments: PGC1(1-550) was cloned downstream of the Gal4 DNA binding domain.
Proper citation: RRID:Addgene_8894 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX Gal4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9529258
Comments: full length PGC1 was cloned downstream of the Gal4 DNA binding domain.
Proper citation: RRID:Addgene_8892 Copy
Species: Mus musculus
Genetic Insert: PPAR gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748014
Proper citation: RRID:Addgene_8896 Copy
Species: Mus musculus
Genetic Insert: Fmr1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16319129
Proper citation: RRID:Addgene_87929 Copy
Species: Mus musculus
Genetic Insert: Trp53
Vector Backbone Description: Vector Backbone:pSpCas9n(BB)-2A-GFP (PX461); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27889317
Proper citation: RRID:Addgene_88847 Copy
Species: Mus musculus
Genetic Insert: Fmr1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16319129
Proper citation: RRID:Addgene_87913 Copy
Species: Mus musculus
Genetic Insert: Bad
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11493700
Proper citation: RRID:Addgene_8799 Copy
Species: Mus musculus
Genetic Insert: Bad
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11493700
Proper citation: RRID:Addgene_8797 Copy
Species: Mus musculus
Genetic Insert: PPAR gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3160; Vector Backbone:pSV-Sport; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7926726
Proper citation: RRID:Addgene_8862 Copy
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