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Species: Homo sapiens
Genetic Insert: ETV3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3000; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257861
Comments: Represents the ORFeome subset of the TFome
Proper citation: RRID:Addgene_88804 Copy
Species: Synthetic
Genetic Insert: toxin-antitoxin + LuxABCDE
Vector Backbone Description: Backbone Marker: Nobuhiko Okada (PMID: 9846743); Vector Backbone:pLZ12Km2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24061415
Comments: This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784
Proper citation: RRID:Addgene_88902 Copy
Species: Synthetic
Genetic Insert: toxin-antitoxin + Red-shifted firefly luciferase
Vector Backbone Description: Backbone Marker: Nobuhiko Okada (PMID: 9846743); Vector Backbone:pLZ12Km2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24061415
Comments: This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784
Proper citation: RRID:Addgene_88901 Copy
Species: Synthetic
Genetic Insert: C-BLa 1.2 fusion protein
Vector Backbone Description: Backbone Marker:John E. Cronan; Backbone Size:4232; Vector Backbone:pBAD322K (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28266525
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_89000 Copy
Species: Other
Genetic Insert: OsPDS-gRNA02
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89263 Copy
Species: Other
Genetic Insert: OsDEP1-gRNA02
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89267 Copy
Species: Other
Genetic Insert: OsDEP1-gRNA01
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89266 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Comments: p35s::Cas9::RZ::gRNA::RZ
Proper citation: RRID:Addgene_89257 Copy
Vector Backbone Description: Vector Backbone:pTX171; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Comments: pZmUbi1::Cas9::RZ cleavage site::gRNA::RZ cleavage site::RZ
Proper citation: RRID:Addgene_89259 Copy
Species: Homo sapiens
Genetic Insert: Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28346450
Proper citation: RRID:Addgene_89157 Copy
Species: Homo sapiens
Genetic Insert: Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28346450
Proper citation: RRID:Addgene_89155 Copy
Species: HIV
Genetic Insert: IN
Vector Backbone Description: Backbone Marker:Warner Greene; Backbone Size:5000; Vector Backbone:pEGFP-PK; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:13130095
Comments: Generates alternate linker (Gly-Ser-Ser-Gly) in between PK and IN to compare to results of pPS2986. Localizes to cytoplasm.
Proper citation: RRID:Addgene_8929 Copy
Species: HIV
Genetic Insert: IN
Vector Backbone Description: Backbone Marker:Warner Greene; Backbone Size:5000; Vector Backbone:pEGFP-PK; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:13130095
Comments: Generates pEGFP-PK-codon optimized IN fusion. Localizes to cytoplasm.
Proper citation: RRID:Addgene_8928 Copy
Species: Rattus norvegicus
Genetic Insert: rAPOBEC1-nCas9 (D10A)-UGI-NLS
Vector Backbone Description: Backbone Size:5379; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28244995
Proper citation: RRID:Addgene_89508 Copy
Species: Synthetic
Genetic Insert: Asparaginyl endopeptidase from Oldenlandia affinis
Vector Backbone Description: Backbone Size:4540; Vector Backbone:pHYRSF53; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89482 Copy
Species: synthetic construct
Genetic Insert: glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5240; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_89593 Copy
Species: synthetic construct
Genetic Insert: glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5240; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_89595 Copy
Species: Homo sapiens
Genetic Insert: Optop38i dark-state mutant
Vector Backbone Description: Backbone Marker:Derived by our lab using pExFP-C1 and pGL3; Backbone Size:5673; Vector Backbone:pLuc-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.
Proper citation: RRID:Addgene_89748 Copy
Species: Synthetic
Genetic Insert: OptoJNKi
Vector Backbone Description: Backbone Marker:Derived by our lab using pExFP-C1 and pGL3; Backbone Size:5673; Vector Backbone:pLuc-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: Please see
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017 in press.
Proper citation: RRID:Addgene_89744 Copy
Species: Synthetic
Genetic Insert: NLS-OptoJNKi dark-state mutant
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression, Fluorescent protein; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.
Proper citation: RRID:Addgene_89743 Copy
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