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Species: Pisum sativum
Genetic Insert: rbcS-E9 enhancer
Vector Backbone Description: Backbone Marker:Subramaniam, Pan, and Cluzel, 2013; Backbone Size:4357; Vector Backbone:pZS*11-yfp0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32409318
Proper citation: RRID:Addgene_149423 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_149306 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_149309 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP12
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_149314 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF3A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_149319 Copy
Species: Synthetic
Genetic Insert: SENP_EuB protease
Vector Backbone Description: Backbone Marker:Görlich lab; Backbone Size:3997; Vector Backbone:pDG-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31023724
Proper citation: RRID:Addgene_149333 Copy
Species: E. coli
Genetic Insert: Biotin ligase BirA
Vector Backbone Description: Backbone Marker:Görlich lab; Backbone Size:4278; Vector Backbone:pDG-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26633879
Proper citation: RRID:Addgene_149334 Copy
Species: Synthetic
Genetic Insert: LuxR-mRFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149482 Copy
Species: Homo sapiens
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:860; Vector Backbone:AmpR gene, with start and stop codons; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2.
Please note: Addgene NGS finds that the AmpR resistance marker is in the opposite orientation in the plasmid relative to the depositor's sequence. As the plasmid was able to grow in media with Ampicillin, this is not expected to affect plasmid function.
Proper citation: RRID:Addgene_149539 Copy
Species: Homo sapiens
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:860; Vector Backbone:AmpR gene, with start and stop codons; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2.
Please note: Addgene NGS finds that the AmpR resistance marker is in the opposite orientation in the plasmid relative to the depositor's sequence. As the plasmid was able to grow in media with Ampicillin, this is not expected to affect plasmid function.
Proper citation: RRID:Addgene_149543 Copy
Species: Synthetic
Genetic Insert: ERT2-Cre-ERT2 fusion protein
Vector Backbone Description: Backbone Marker:Liqun Luo Lab (Addgene Plasmid #40026); Backbone Size:14483; Vector Backbone:pROSA26-TG; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33082936
Proper citation: RRID:Addgene_149436 Copy
Vector Backbone Description: Backbone Size:4493; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.77, Ligation number NONE.
Proper citation: RRID:Addgene_1495 Copy
Species: Homo sapiens
Genetic Insert: Rheb1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:HA-GST-pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17386266
Proper citation: RRID:Addgene_14951 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Tropix, Bedford, MA; Backbone Size:7114; Vector Backbone:pSEAP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17079315
Comments: The vector includes 5' and 3' genomic regions of the human K18 gene, necessary for driving high-level epithelial-cell-specific expression
Proper citation: RRID:Addgene_149449 Copy
Species: Synthetic
Genetic Insert: LuxR-mRFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149465 Copy
Genetic Insert: iSpyMacCas9
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible ziSpyMacCas9 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149553 Copy
Genetic Insert: hAID-ziSpyMacCas9(D10A)-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149554 Copy
Genetic Insert: ziSpyMacCas9(D10A)-PmCDA1-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149555 Copy
Vector Backbone Description: Backbone Size:7562; Vector Backbone:pBigT; Vector Types:Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657333
Comments: Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.
Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.
Proper citation: RRID:Addgene_15037 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' and 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6207484
Comments: Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.
Proper citation: RRID:Addgene_15030 Copy
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