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Species: Other
Genetic Insert: dropout part, lv0*, 1R-5O, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250041 Copy
Species: Other
Genetic Insert: Ba1Cas12a3 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253635 Copy
Species: Other
Genetic Insert: Sm3Cas12a3 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253637 Copy
Species: Other
Genetic Insert: ApCas12a4 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253639 Copy
Species: Other
Genetic Insert: ABR, lv0, Atet, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249981 Copy
Species: Other
Genetic Insert: ABR, lv0*, Atet, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250022 Copy
Species: Other
Genetic Insert: MpCas12a E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253634 Copy
Species: Other
Genetic Insert: Surface marker and Citrine activation reporter
Vector Backbone Description: Vector Backbone:pAAVS1 donor; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33326746
Comments: Cloned with reagents shared by and in collaboration with Michael Bassik.
Proper citation: RRID:Addgene_161928 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Connie Cepko (Addgene plasmid # 11151); Backbone Size:4878; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The original multiple cloning site was replaced by an extended MCS to improve usability of the plasmid for restriction cloning.
Proper citation: RRID:Addgene_164092 Copy
Species: Other
Genetic Insert: Split GFP(beta1-10)
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33058229
Proper citation: RRID:Addgene_164875 Copy
Species: Other
Genetic Insert: hAPOBEC3A fused to PspCas13b
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33058229
Proper citation: RRID:Addgene_164872 Copy
Species: Other
Genetic Insert: gRNA for TC1405
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33058207
Proper citation: RRID:Addgene_164878 Copy
Species: Other
Genetic Insert: dcas13d-ADARdd
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33058207
Proper citation: RRID:Addgene_164881 Copy
Species: Other
Genetic Insert: dcas13d-ADARdd
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33058207
Proper citation: RRID:Addgene_164883 Copy
Species: Other
Genetic Insert: mCardinal
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166236 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8594; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169741 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:13296; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Please note the OriP sequence contains a deletion that is also found in Addgene plasmid (pCEP4-CXCR4) which was used as the backbone.
Proper citation: RRID:Addgene_169742 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6113; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169740 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5051; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169739 Copy
Species: Other
Genetic Insert: gIII, luxAB, guide RNA
Vector Backbone Description: Vector Backbone:pBT120; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-I9q2W3bzpjsuOoxQPyCj for additional information.
Proper citation: RRID:Addgene_122604 Copy
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