Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Drosophila melanogaster
Genetic Insert: Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28395731
Comments: To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCherry: Clonetech
Proper citation: RRID:Addgene_163926 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_163992 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_163997 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_163996 Copy
Vector Backbone Description: Backbone Size:30767; Vector Backbone:pAdEasy-2; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9482916
Comments: Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-2.
Plasmids recombined in pAdEasy-2 should be packaged in 911E4 cells to make virus.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16401 Copy
Genetic Insert: PGK-NeoR & ZeoR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: Part of vector system to facilitate and simplify the use of rAAV for creating KOs in human cells. The other vectors for this system, pAAV-MCS, pAAV-RC, and pHelper, can be purchased from Stratagene.
The sequence information is the sequence between linkers A and B.
See author's map and article for more information. In the author's map, restriction sites for the generation of pNeDaKO fragments used for fusion PCR are shown. A and B refer to linkers A and B; yellow arrows: loxP sites; PGK: phosphoglycerate kinase eukaryotic promoter; Neo: neomycin resistance gene; EM 7: EM7 prokaryotic promoter; Zeo: zeomycin resistance gene; Amp: ampicillin resistance gene.
Proper citation: RRID:Addgene_16413 Copy
Species: Homo sapiens
Genetic Insert: PTEN Neo targeting
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4000; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: This targeting vector yields 1-5% homologous recombinants in HCT116 and other cell lines. See author's map for more information.
Proper citation: RRID:Addgene_16411 Copy
Species: Mus musculus
Genetic Insert: MeCP2 Isoform 1
Vector Backbone Description: Vector Backbone:pAAV-EF1a; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31868585
Proper citation: RRID:Addgene_164062 Copy
Species: Homo sapiens
Genetic Insert: CLGT#2-mCherry
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9598; Vector Backbone:FUGW; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33361384
Comments: From TCGA datasets, we annotated the genes specific to classical glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.
Proper citation: RRID:Addgene_164106 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:7700; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164134 Copy
Species: Synthetic
Genetic Insert: NFkB promoter-Cre-T2A-mRuby3
Vector Backbone Description: Backbone Size:8000; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164137 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:7700; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164135 Copy
Species: Homo sapiens
Genetic Insert: MGT#2-mCherry
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14884; Backbone Size:7956; Vector Backbone:FUGW; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33361384
Comments: From TCGA datasets, we annotated the genes specific to mesenchymal glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.
Proper citation: RRID:Addgene_164097 Copy
Species: Homo sapiens
Genetic Insert: MGT#1-mVenus
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:7956; Vector Backbone:FUGW; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33361384
Comments: From TCGA datasets, we annotated the genes specific to mesenchymal glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.
Proper citation: RRID:Addgene_164094 Copy
Species: Mus musculus
Genetic Insert: Hq-ERV
Vector Backbone Description: Backbone Marker:Invitrogen/ThermoFisher; Backbone Size:3929; Vector Backbone:pCR-XL-2-TOPO; Vector Types:eukaryotic expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33367954
Proper citation: RRID:Addgene_164089 Copy
Vector Backbone Description: Backbone Size:10183; Vector Backbone:lentiGuide-Puro (Addgene #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164124 Copy
Species: Synthetic
Genetic Insert: PP7-CFP
Vector Backbone Description: Vector Backbone:pSC101-Amp; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33674569
Proper citation: RRID:Addgene_164128 Copy
Species: Synthetic
Genetic Insert: LgBiT-PhoCl1-SmBiT-MBP
Vector Backbone Description: Backbone Size:4064; Vector Backbone:pBad/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34349937
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.10.419556v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_164034 Copy
Species: Synthetic
Genetic Insert: dCas9-14xGFP11
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30498221
Proper citation: RRID:Addgene_164038 Copy
Genetic Insert: PhoCl2f
Vector Backbone Description: Backbone Size:4076; Vector Backbone:pBad/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34349937
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.10.419556v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_164036 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
