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Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Marker:Addgene plasmid # 39280; Backbone Size:2300; Vector Backbone:pFA6a-kanMX6-P3nmt1; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18197241
Proper citation: RRID:Addgene_39349 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Notch1 (clone B9)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22842086
Comments: Binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Proper citation: RRID:Addgene_39344 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Desmin (clone D7)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39346 Copy
Species: Homo sapiens
Genetic Insert: scFv specific for Jagged (clone D5)
Vector Backbone Description: Vector Backbone:pBIOCAM5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39345 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39336 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39338 Copy
Species: Helicobacter pylori
Genetic Insert: oxidoreductase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This construct can be expressed in BL21(DE3) cells.
Proper citation: RRID:Addgene_39334 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39296 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: 1nt mismatch at bp#102 which may affect NheI site. Should not effect plasmid function.
Proper citation: RRID:Addgene_39295 Copy
Species: synthetic gene
Genetic Insert: MSP1E3D1_D73C
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19903557
Proper citation: RRID:Addgene_39328 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Do not cut out EB1 using EcoR1 as there is an internal EcoR1 site in the EB1 sequence.
Proper citation: RRID:Addgene_39324 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39285 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39284 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: Use GST reverse primer to verify mutation in nmt1 promoter
Proper citation: RRID:Addgene_39286 Copy
Species: Neisseria meningitidis
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:pET-based custom vector; Backbone Size:6400; Vector Backbone:pEC-K-MBP; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22745249
Proper citation: RRID:Addgene_39317 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39280 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: 7bp deletion (ATATAAA), 2nt mismatch at bp#4267-4268 and 7nt deletion at bp#4269-4275 when compared to EU493333.1. Does not effect function.
Proper citation: RRID:Addgene_39282 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39307 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39303 Copy
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