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Species: Homo sapiens
Genetic Insert: Brca1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10550055
Comments: pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro.
The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.
Proper citation: RRID:Addgene_14999 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (-109/+334)
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pBV-luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158583
Comments: XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid.
Proper citation: RRID:Addgene_14969 Copy
Genetic Insert: Cre-ER(TM)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
See Author's Map for more information.
Proper citation: RRID:Addgene_15028 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.
See Author's Map for more information.
Proper citation: RRID:Addgene_15021 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149663 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149668 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Streptomycin
Comments: BC039902
Proper citation: RRID:Addgene_149719 Copy
Vector Backbone Description: Backbone Size:4492; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.81, Ligation number NONE.
Proper citation: RRID:Addgene_1497 Copy
Vector Backbone Description: Backbone Size:4470; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.85, Ligation number NONE.
Proper citation: RRID:Addgene_1498 Copy
Species: Homo sapiens
Genetic Insert: LA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5933; Vector Backbone:pTracer-EF/V5-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15003 Copy
Species: Rattus norvegicus
Genetic Insert: p130cas
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11314030
Proper citation: RRID:Addgene_15001 Copy
Species: HIV-1
Genetic Insert: Capsid
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:2863; Vector Backbone:pTZ19U; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33033190
Proper citation: RRID:Addgene_149687 Copy
Species: Homo sapiens
Genetic Insert: HNF4A-FOXA2-FOXA3
Vector Backbone Description: Backbone Marker:GeneCopoeia; Backbone Size:5784; Vector Backbone:CmiR0001-MR03; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33461059
Proper citation: RRID:Addgene_149724 Copy
Species: Mus musculus
Genetic Insert: IFT88
Vector Backbone Description: Backbone Size:9800; Vector Backbone:pEF1α-IRES-EGFP (IRES-EGFP removed); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_149697 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8455633
Comments: In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(A) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').
Proper citation: RRID:Addgene_15160 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:1731342
Comments: Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.
Proper citation: RRID:Addgene_15164 Copy
Vector Backbone Description: Backbone Size:4835; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD99.44, Ligation number L2680.
Proper citation: RRID:Addgene_1516 Copy
Species: Mus musculus
Genetic Insert: Notch1 (C-term)
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006984
Comments: mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus.
Proper citation: RRID:Addgene_15131 Copy
Vector Backbone Description: Backbone Size:4919; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.78, Ligation number L2603.
Proper citation: RRID:Addgene_1511 Copy
Vector Backbone Description: Backbone Size:4702; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.82, Ligation number L2604.
Proper citation: RRID:Addgene_1512 Copy
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