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Species: Homo sapiens
Genetic Insert: NRF2
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TT miRc2 3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136527 Copy
Species: Homo sapiens
Genetic Insert: CHEK2
Vector Backbone Description: Backbone Marker:Addgene #25735; Backbone Size:13290; Vector Backbone:pSLIK-Neo; Vector Types:Mammalian Expression, Lentiviral, destinatioin vector for gateway cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136536 Copy
Species: Homo sapiens
Genetic Insert: Beclin-1 IDR + CC
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29185708
Proper citation: RRID:Addgene_136686 Copy
Species: Homo sapiens
Genetic Insert: ZNF8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562
Proper citation: RRID:Addgene_136689 Copy
Species: Homo sapiens
Genetic Insert: Beclin-1
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29185708
Comments: Large-scale expression of the BECN1 fusion protein was performed by growing cultures in TB at 37 °C with orbital shaking at 180 rpm to mid-log phase (A600 ∼ 0.6−0.8), cooling cultures to 18 °C for 30 min before induction with 0.5 mM isopropyl β-D-1-thiogalactopyranoside, and growing the induced cultures for 18 h at 18 °C. Cell pellets were harvested via centrifugation and stored at −50 °C.
See Ranaghan et al. (2017) [DOI: 10.1021/acs.biochem.7b00936] for purification methods.
Proper citation: RRID:Addgene_136688 Copy
Species: Homo sapiens
Genetic Insert: shJUNDv2
Vector Backbone Description: Backbone Marker:Addgene # 21915; Backbone Size:10633; Vector Backbone:tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136583 Copy
Species: Homo sapiens
Genetic Insert: shNRF2v1
Vector Backbone Description: Backbone Marker:Addgene # 21915; Backbone Size:10633; Vector Backbone:tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136584 Copy
Species: Homo sapiens
Genetic Insert: shNRF2v2
Vector Backbone Description: Backbone Marker:Addgene # 21915; Backbone Size:10633; Vector Backbone:tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136585 Copy
Species: Homo sapiens
Genetic Insert: HPV16E7∆DLYC
Vector Backbone Description: Backbone Size:5874; Vector Backbone:pLXSN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136588 Copy
Species: Homo sapiens
Genetic Insert: SH3BP1 (RNAi resistant)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA4-TO-mychis; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22891260
Proper citation: RRID:Addgene_136645 Copy
Species: Homo sapiens
Genetic Insert: SH3BP1-CTD
Vector Backbone Description: Vector Backbone:pGEX-4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22891260
Proper citation: RRID:Addgene_136649 Copy
Species: Homo sapiens
Genetic Insert: St1Cas9 LMD-9:CNRZ_1066 Chimera
Vector Backbone Description: Vector Backbone:MSP1594_2x_NLS (Plasmid #110625); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31900288
Proper citation: RRID:Addgene_136651 Copy
Species: Homo sapiens
Genetic Insert: AcrIIA5_D1126
Vector Backbone Description: Vector Backbone:pVAX_CBI; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30046034
Proper citation: RRID:Addgene_136663 Copy
Species: Homo sapiens
Genetic Insert: AcrIIA5_D4276
Vector Backbone Description: Vector Backbone:pVAX_CBI; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30046034
Proper citation: RRID:Addgene_136664 Copy
Species: Homo sapiens
Genetic Insert: AcrIIA6_D1024
Vector Backbone Description: Vector Backbone:pVAX_CBI; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30046034
Proper citation: RRID:Addgene_136666 Copy
Species: Homo sapiens
Genetic Insert: AcrIIA6_DT1
Vector Backbone Description: Vector Backbone:pVAX_CBI; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30046034
Proper citation: RRID:Addgene_136668 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 E7 del EDE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9284049
Proper citation: RRID:Addgene_13675 Copy
Species: Homo sapiens
Genetic Insert: CLIP-hSfi1
Vector Backbone Description: Backbone Size:10957; Vector Backbone:pEBTetBl; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23333316
Proper citation: RRID:Addgene_136842 Copy
Species: Homo sapiens
Genetic Insert: CLIP-FGFR
Vector Backbone Description: Backbone Size:10943; Vector Backbone:pEBTetBl; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23333316
Proper citation: RRID:Addgene_136845 Copy
Species: Homo sapiens
Genetic Insert: CLIP-Cep97
Vector Backbone Description: Backbone Size:10945; Vector Backbone:pEBTetBl; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23333316
Proper citation: RRID:Addgene_136849 Copy
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