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Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_164020 Copy
Species: Homo sapiens
Genetic Insert: LMNA targeting sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33104788
Proper citation: RRID:Addgene_164045 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:7451; Vector Backbone:pQCXIZ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164232 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:7451; Vector Backbone:pQCXIZ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164233 Copy
Species: Homo sapiens
Genetic Insert: RPA70
Vector Backbone Description: Backbone Size:7879; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164231 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8367; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164237 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8312; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164235 Copy
Species: Synthetic
Genetic Insert: CamPARI2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4579; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33762909
Proper citation: RRID:Addgene_164193 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8371; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164239 Copy
Species: Homo sapiens
Genetic Insert: GLI
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2832761
Comments: GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).
Proper citation: RRID:Addgene_16419 Copy
Species: Homo sapiens
Genetic Insert: ACE2 extracellular domain
Vector Backbone Description: Backbone Marker:Addgene plasmids 12154; Vector Backbone:pCSCG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33103998
Proper citation: RRID:Addgene_164222 Copy
Species: SARS-CoV
Genetic Insert: Spike S1 domain
Vector Backbone Description: Backbone Marker:Addgene plasmids 12154; Vector Backbone:pCSCG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33103998
Proper citation: RRID:Addgene_164220 Copy
Genetic Insert: PGK-HygR & ZeoR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: Part of vector system to facilitate and simplify the use of rAAV for creating KOs in human cells. The other vectors for this system, pAAV-MCS, pAAV-RC, and pHelper, can be purchased from Stratagene.
The sequence information is the sequence between linkers A and B.
See author's map and article for more information. In the author's map, restriction sites for the generation of pNeDaKO fragments used for fusion PCR are shown. A and B refer to linkers A and B; yellow arrows: loxP sites; PGK: phosphoglycerate kinase eukaryotic promoter; Hyg: hygromycin resistance gene; EM 7: EM7 prokaryotic promoter; Zeo: zeomycin resistance gene; Amp: ampicillin resistance gene.
Note that though the Hyg-R gene has a point mutation, the Vogelstein lab has used this construct to for Hyg selection extensively.
Proper citation: RRID:Addgene_16414 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8370; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164229 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8370; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164228 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT2; Vector Types:Mammalian Expression, Cre/Lox, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164138 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:6732; Vector Backbone:pQCXIZ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164243 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8371; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164241 Copy
Species: Homo sapiens
Genetic Insert: LMNB1
Vector Backbone Description: Backbone Size:7879; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164249 Copy
Species: Mus musculus
Genetic Insert: anti-GABA(A)R, alpha6 (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: Isotype: IgG2a.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_164155 Copy
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