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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pS-sg:GFP Resource Report Resource Website |
RRID:Addgene_196296 | Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs | Synthetic | Kanamycin | PMID:36751129 | Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:07 | 0 | ||
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kiCAP-AAV-PAL2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_196691 | AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589 | Synthetic | Kanamycin | PMID:36417917 | Backbone Size:3000; Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin | PTQGTVR insert between amino acids 588 and 589 of AAV9 VP1, S586E, A587V, Q588G | 2026-08-15 01:26:07 | 1 | |
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VPS13D1-1576-(Y768A) Resource Report Resource Website |
RRID:Addgene_194006 | Codon optimized VPS13D | Homo sapiens | Kanamycin | PMID:33891013 | Backbone Marker:Genscript; Backbone Size:6000; Vector Backbone:pcDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:07 | 0 | ||
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JAB2599 Resource Report Resource Website |
RRID:Addgene_190386 | proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt | Synthetic | Kanamycin | PMID:35951698 | Backbone JAB2155 contains proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint. | Vector Backbone:JAB2155; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:07 | 0 | |
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pCB301-2μ-HDV Resource Report Resource Website |
RRID:Addgene_196329 | Kanamycin | PMID:29112135 | Vector Backbone:pCB301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:09 | 0 | ||||
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VR122 pETv2 10His-pG-Tn5 (E54K, L372P) Resource Report Resource Website 1+ mentions |
RRID:Addgene_198467 | 10His-pG-Tn5 (E54K, L372P) | Synthetic | Kanamycin | PMID:39026714 | Please visit https://www.biorxiv.org/content/10.1101/2024.07.11.602973v1 for bioRxiv preprint. | Backbone Size:5234; Vector Backbone:pETv2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | E54K, L372P | 2026-08-15 01:26:09 | 1 |
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pL Resource Report Resource Website |
RRID:Addgene_196286 | Full length TSWV L antigenome encoding codon-optimized L (RdRp) | Synthetic | Kanamycin | PMID:36751129 | Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:08 | 0 | ||
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pM-CBE Resource Report Resource Website |
RRID:Addgene_196293 | Full length TSWV M antigenome encoding CBE in place of viral GP | Synthetic | Kanamycin | PMID:36751129 | Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:08 | 0 | ||
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pMUT2-VtrA-mCherry Resource Report Resource Website |
RRID:Addgene_192862 | VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids | Vibrio cholerae | Kanamycin | PMID:36449712 | Backbone Size:5601; Vector Backbone:pMUT2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:09 | 0 | ||
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pUS250 Resource Report Resource Website 1+ mentions |
RRID:Addgene_198322 | Kanamycin | CLONING: The multiple cloning site (MCS) in pUS250 functions differently to a typical MCS. You need to choose one unique restriction site on the left side of the amilCP marker gene (e.g. EcoRI), and one unique restriction site on the right side (e.g. PstI) in order for the blue/white selection and the cumate-inducible expression features to work properly. Cutting the plasmid in this way excises the amilCP gene, replacing it with your gene of interest, and changing the phenotype from blue to white. The vector is also compatible with GoldenGate cloning (using either BsaI or Esp3I) and BioBrick cloning (iGEM). In the case of GoldenGate cloning, you don't need to use two different enzymes, you just use one or the other, since each enzyme has two sites, one on each side of amilCP, and each yields a different overhang. HOST RANGE and EXPRESSION: we have shown that pUS250 can be used for cumate-inducible gene expression in E.coli, Pseudomonas putida, and Rhizobium leguminosarum. It is likely that the plasmid will also be useful in other gram negatives (Proteobacteria) since the pBBR replicon has a very broad host range. The plasmid can be transferred by conjugation from E.coli strains such as S17 or SM10 into other species due to the oriT sequence. For the E.coli and Rhizobium, 100 uM cumate is sufficient for expression. For Pseudomonas, this needs to be increased to 10 mM (we think there is an efflux pump that pumps cumate back out of these cells). The cumate should be added after autoclaving. We make a 0.5 M cumate stock solution by mixing equal parts of 1M aqueous Tris base with 1M cumic acid in ethanol. Cumate is an excellent inducer since it is both cheap and non-toxic to both bacteria and people. You can even use cumin (the spice) for induction of gene expression in this plasmid! (there is enough cumate in the cumin). STABILITY and COPY NUMBER: We estimate that the vector has a copy number of 5-10 in E.coli, so it is certainly a low copy vector. Best to make large-scale plasmid preps (e.g. 50 ml culture) rather than small-scale, in order to ensure you get enough plasmid DNA to work with. The plasmid is quite stable but we have seen white mutants appear occasionally which have deletions in amilCP. | Backbone Marker:Coleman; Backbone Size:4690; Vector Backbone:pUS250; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:09 | 2 | ||||
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pET30_Halotag_K73T_v2 Resource Report Resource Website |
RRID:Addgene_183692 | ER Halo(K73T) | Bacterial | Kanamycin | PMID:35523806 | Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | K73T in Halotag | 2026-08-15 01:26:10 | 0 | |
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P1_Firre_1.49_pDONR221 Resource Report Resource Website |
RRID:Addgene_195204 | mFirre 1.49 Clone | Mus musculus | Kanamycin | PMID:24463464 | Backbone Size:4761; Vector Backbone:pDONR221; Vector Types:Gateway Cloning Intermediate; Bacterial Resistance:Kanamycin | Only exons | 2026-08-15 01:26:10 | 0 | |
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pMUT2-pLac-mCherry Resource Report Resource Website |
RRID:Addgene_192859 | lldR operon | Escherichia coli | Kanamycin | PMID:36449712 | Backbone Size:4866; Vector Backbone:pMUT2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:08 | 0 | ||
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pMUT2-pAto-mCherry Resource Report Resource Website |
RRID:Addgene_192858 | Acetoacetate inducible promoter | Escherichia coli | Kanamycin | PMID:36449712 | Backbone Size:5591; Vector Backbone:pMUT2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:09 | 0 | ||
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L0-I15 tUBQ1 GG4-GG6 Resource Report Resource Website |
RRID:Addgene_195897 | tUBQ1 | Arabidopsis thaliana | Kanamycin | PMID:37012288 | Backbone Marker:Thermo Fisher Scientific; Backbone Size:3512; Vector Backbone:pCR-BluntII-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:15 | 0 | ||
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L0-I16 tUBQ1 GG5-GG6 Resource Report Resource Website |
RRID:Addgene_195898 | tUBQ1 | Arabidopsis thaliana | Kanamycin | PMID:37012288 | Backbone Marker:Thermo Fisher Scientific; Backbone Size:3512; Vector Backbone:pCR-BluntII-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:15 | 0 | ||
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pEGFPN3 FRB Resource Report Resource Website |
RRID:Addgene_194120 | FRB domain of mTOR | Homo sapiens | Kanamycin | Vector Backbone:pEGFPN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:15 | 0 | |||
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L0-I40 Intein-PhiC31 GG1-GG4 Resource Report Resource Website |
RRID:Addgene_195906 | Intein-PhiC31 | Synthetic | Kanamycin | PMID:37012288 | Backbone Marker:Thermo Fisher Scientific; Backbone Size:3512; Vector Backbone:pCR-BluntII-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:15 | 0 | ||
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pGBT-RP8.3 Resource Report Resource Website |
RRID:Addgene_189848 | Kanamycin | PMID:32779569 | Backbone Size:5984; Vector Backbone:pGBT; Vector Types:Cre/Lox; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:15 | 0 | ||||
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tl1-FusXTBE-Left TALE Resource Report Resource Website |
RRID:Addgene_196506 | IDH2 MTS-tl1 left TALE-G1397 DddA-C-UGI-SV40 polyA | Burkholderia cenocepacia | Kanamycin | PMID:34847747 | Vector Backbone:pT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:19 | 0 |
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