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Species: Gallus gallus
Genetic Insert: Tensin
Vector Backbone Description: Backbone Marker:Yamada Lab, Tamura et al., Science 280: 1614-7 (1998); Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10704455
Proper citation: RRID:Addgene_50514 Copy
Species: Mus musculus
Genetic Insert: protein tyrosine kinase
Vector Backbone Description: Backbone Marker:Yamada Lab, Tamura et al., Science 280: 1614-7 (1998); Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10427092
Comments: Fluorophore encoded by this plasmid is ABB59962.1 with G66A.
Proper citation: RRID:Addgene_50515 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Backbone Marker:Yamada lab ; Vector Backbone:pCFP NBC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Vector is a Clontech C1 vector with an MCS modification with original Bam HI mutation
Proper citation: RRID:Addgene_50510 Copy
Genetic Insert: hM4D(Gi)-mCherry
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50479 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Yamada Lab, Tamura et al., Science 280: 1614-7 (1998); Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9616126
Proper citation: RRID:Addgene_50519 Copy
Species: Mus musculus
Genetic Insert: nAChRBeta3 subunit, isoform 1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5597; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17932221
Proper citation: RRID:Addgene_50484 Copy
Species: Mus musculus
Genetic Insert: protein tyrosine kinase, domain
Vector Backbone Description: Backbone Marker:Bruce Howard Lab, PubMed ID 1373145; Vector Backbone:pCMVIL2R(pIL2R); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1373145
Comments: Vector contains truncated IL-2R alpha subunit without cytoplasmic domain (Tac antigen) for membrane localization and clustering
Proper citation: RRID:Addgene_50524 Copy
Species: Gallus gallus
Genetic Insert: Vinculin
Vector Backbone Description: Backbone Marker:Yamada Lab; Vector Backbone:pmCherry NBC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Replacement of Clontech pEGFP C1 vector EGFP with mCherry and MCS modification with Bam HI mutation of original Bam H I.
Proper citation: RRID:Addgene_50527 Copy
Species: Homo sapiens
Genetic Insert: PXN paxillin
Vector Backbone Description: Backbone Marker:Yamada Lab; Vector Backbone:pmCherry NBC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Replacement of Clontech pEGFP C1 vector EGFP with mCherry and MCS modification with Bam HI mutation of original Bam H I.
Proper citation: RRID:Addgene_50526 Copy
Species: Homo sapiens
Genetic Insert: TRF2
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4378; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25115914
Comments: This set of TRF2 and TRF2 mutant vectors has been engineered reintroduce Alanine 434, which is deleted in most published TRF2 vectors, and to generate TRF2 and TRF2 mutant proteins with identical N-terminal tags.
Proper citation: RRID:Addgene_50488 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Yamada Lab, Tamura et al., Science 280: 1614-7 (1998); Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9616126
Proper citation: RRID:Addgene_50520 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Yamada Lab, Tamura et al., Science 280: 1614-7 (1998); Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9616126
Proper citation: RRID:Addgene_50523 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Backbone Marker:Yamada Lab, Tamura et al., Science 280: 1614-7 (1998); Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50529 Copy
Species: Homo sapiens
Genetic Insert: fibronectin
Vector Backbone Description: Vector Backbone:pVC70108; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929152
Comments: Primers used for amplification of insert: primer A (CCATATGGGTCGACCCAACTGG), which contains an NdeI site, methionine start codon, and 5' end of the 9th type III repeat sequence, and primer B (ATGAATTCTACATCTGGGATGGTTTGTCAA), which contains an EcoRI site, a stop codon, and the 3' end of the 37-kDa fibronectin fragment.
Proper citation: RRID:Addgene_50495 Copy
Species: Homo sapiens
Genetic Insert: NM_001306129.1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETC'; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7545166
Proper citation: RRID:Addgene_50497 Copy
Species: Homo sapiens
Genetic Insert: fibronectin
Vector Backbone Description: Vector Backbone:pVC70108; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50496 Copy
Species: Homo sapiens
Genetic Insert: fibronectin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETC'; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50498 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:PMID 14729065; Vector Backbone:pRKVSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50539 Copy
Species: Other
Genetic Insert: MitoTimer
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2516; Vector Backbone:pTRE Tight; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24128932
Comments: The F12S mutation in Timer does not affect the function of the plasmid, as described in the associated publication.
The Timer sequence is preceded by the mitochondrial targeting sequence from cytochrome c oxidase subunit 8A: MSVLTPLLLRGLTGSARRLPVPRAKIHSL
Proper citation: RRID:Addgene_50547 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Registry of Standard Biological Parts; Backbone Size:3296; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24608181
Comments: Note: Addgene's quality control sequencing found a few mismatches compared to the depositor's full sequence, but they should not affect plasmid function.
Proper citation: RRID:Addgene_50549 Copy
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